FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8644505
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103270711.946398318932085No Hit
CAACGGAATCCCAAAAGCAGCTG6824647.894772459498838No Hit
CCCCCACTGCTAAATTTGACTG3336203.859330291323795No Hit
AACATTCAACGCTGTCGGTGAGT3232453.739311851864277No Hit
TAGCAGCACGTAAATATTGGCG2344292.7118846018366582No Hit
TTCAAGTAATCCAGGATAGGCT2222392.570870165498198No Hit
AACATTCAACGCTGTCGGTGAG2150702.4879388698369658No Hit
AAGCTGCCAGTTGAAGAACTGT1845382.1347434005764354No Hit
TATTGCACTTGTCCCGGCCTGTAA1673691.9361316813397644No Hit
TATTGCACTTGTCCCGGCCTGTAT1577061.8243496880388177No Hit
TAGCTTATCAGACTGATGTTGACT1086701.2570991629942951No Hit
TAGCTTATCAGACTGATGTTGAC986151.1407824970891913No Hit
TATTGCACTTGTCCCGGCCTGT889331.0287807109834513No Hit
TAGCTTATCAGACTGATGTTGA880141.0181496800568683No Hit
TATTGCACTTGTCCCGGCCTGTA772650.8938047927556292No Hit
TGAGGTAGTAGGTTGTATAGTT770080.8908318058697403No Hit
TGTAAACATCCTTGACTGGAAGCT608400.7037996970329706No Hit
AGCAGCATTGTACAGGGCTATGA585720.6775633769660611No Hit
CATAAAGTAGAAAGCACTACT520470.6020819005830872No Hit
ACTGGACTTGGAGTCAGAAGGC482480.5581349076667779No Hit
TGAGGTAGTAGATTGTATAGTT476170.5508354729391677No Hit
TGTAAACATCCCCGACTGGAAGCT468750.5422519855098702No Hit
CCCATAAAGTAGAAAGCACTAC395480.45749293915614603No Hit
TCGTACGACTCTTAGCGG393750.4554916678282909No Hit
GCCTGTCTGAGCGTCGCT367270.42485949166551473No Hit
GGAATACCGGGTGCTGTAGGCTTT358340.41452922984022794No Hit
TTCAAGTAATTCAGGATAGGTT351110.40616553521572374No Hit
CAACGGAATCCCAAAAGCAGCTGT333820.38616438997953034No Hit
CGCCTGTCTGAGCGTCGCT302390.3498060328497699No Hit
CCCCCACTGCTAAATTTGACTGGCTT295040.34130352171697514No Hit
TTCTCACTACTGCACTTGACTAG279250.3230375828344133No Hit
CCCATAAAGTAGAAAGCACT278640.3223319322506031No Hit
GGAATACCGGGTGCTGTAGGCTT276000.31927796906821154No Hit
CATTGCACTTGTCTCGGTCTGA272750.3155183553020098No Hit
AACATTCAACGCTGTCGGTGAGTT247820.2866792257046529No Hit
CTCCCACTGCTTCACTTGACTAG240330.27801476197885244No Hit
CACTAGATTGTGAGCTCCTGGA238800.27624485149814826No Hit
CTTCTCACTACTGCACTTGACTAG238190.2755392009143381No Hit
CACTAGATTGTGAGCTCCTGGAA237060.27423201212793563No Hit
CAACACCAGTCGATGGGCTGT236560.2736536100100584No Hit
ACCCCCCACTGCTAAATTTGACTG225320.26065113040017907No Hit
ATCCCGGACGAGCCCCCA220060.254566340120111No Hit
TATTGCACTTGTCCCGGCCTGTAG210760.24380806072759517No Hit
CCCCCCACTGCTAAATTTGACTGGCT206790.23921554791165026No Hit
CACCCGGCTGTGTGCACATGTGC206000.23830167256540424No Hit
TGAGGTAGTAGTTTGTACAGTT197580.22856138090035233No Hit
AGCTCGGTCTGAGGCCCCTCAGT197330.2282721798414137No Hit
CCCCCCACTGCTAAATTTGACTGG183940.21278257112466245No Hit
TATTGCACTTGTCCCGGCCTGTT179720.20790085724977891No Hit
AACGGAATCCCAAAAGCAGCTG164360.19013234418859148No Hit
ACCGGGTGCTGTAGGCTTA161790.1871593573027027No Hit
CAAAGAATTCTCCTTTTGGGCT158390.18322622290113777No Hit
TGTCAGTTTGTCAAATACCCCAA154960.17925838437250022No Hit
TGTAAACATCCTACACTCTCAGCT151880.1756954273263767No Hit
ACTGGACTTGGAGTCAGAAGGCT145420.16822247196340334No Hit
TTCACAGTGGCTAAGTTCCG141690.16390759216403947No Hit
TAGCTTATCAGACTGATGTTGACA138990.16078422072750262No Hit
TAGCAGCACGTAAATATTGGC135890.157198127596664No Hit
ACTGGACTTGGAGTCAGAAGGCA135120.15630738833513314No Hit
TCGTTTCCCGGGCGGCGCACCA132340.15309147255973593No Hit
AGCAGCATTGTACAGGGCTATGT131840.15251307044185872No Hit
ACCGGGTGCTGTAGGCTT130230.15065061562229415No Hit
AACCGGGCGGAAACACCA129610.14993339699612643No Hit
TGAGGTAGTAGTTTGTGCTGTT128690.14886913709923238No Hit
CTACGCCTGTCTGAGCGTCGCT128630.14879972884508716No Hit
TTCAAGTAATCCAGGATAGGCTT126540.14638200799236045No Hit
CCCCCCACTGCTAAATTTGACTGT125930.14567635740855028No Hit
TATTGCACTTGTCCCGGCCTGTTT124190.14366351803833766No Hit
TGTAAACATCCCCGACTGGAAGC116060.13425869960165446No Hit
TATTGCACTTGTCCCGGCCTGTAAT114110.1320029313419334No Hit
TTCAAGTAATCCAGGATAGGC112240.1298397074210727No Hit
TTCACAGTGGCTAAGTTCTG111570.12906464858311725No Hit
ACTGGACTTGGAGTCAGAAGG109140.12625361429023407No Hit
TACGCCTGTCTGAGCGTCGCT108860.12592970910422285No Hit
ATCCCACCGCTGCCACCA107620.12449527185188741No Hit
TATTGCACTTGTCCCGGCCTGTAAA107510.12436802338595444No Hit
CCCCCCACTGCTAAATTTGACTGTA106740.12347728412442356No Hit
CCCCCCACTGCTAAATTTGACTGA104030.12034234464552915No Hit
CAAAGAATTCTCCTTTTGGGCTT103700.1199605992477302No Hit
CAACGGAATCCCAAAAGCAGCT103350.11955571776521617No Hit
AGCAGCATTGTACAGGGCTATG101840.11780894336922705No Hit
ACCACAGGGTAGAACCACGGAC98700.11417657806895826No Hit
CTCCCACTGCTTCACTTGACTAGCCT96370.11148122419965052No Hit
TTATAAAGCAATGAGACTGATT94140.10890155075391823No Hit
ACCGGGTGCTGTAGGCTTT94120.10887841466920316No Hit
AACATTCAACGCTGTCGGTGA93990.10872803011855509No Hit
CCCATAAAGTAGAAAGCACTA93930.1086586218644098No Hit
TATTGCACTTGTCCCGGCCTGA92170.10662264640948209No Hit
AACATTCAACGCTGTCGGTGAGA91550.10590542778331437No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC91340.10566249889380594No Hit
TGTCAGTTTGTCAAATACCCCA90960.10522291328421927No Hit
GACCCGCGGGCGCTCTCCAGTCCT90570.10477175963227506No Hit
TGAGGTAGTAGGTTGTGTGGTT89960.10406610904846489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)