FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7117764
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6210878.725872338560256No Hit
CAACGGAATCCCAAAAGCAGCTG5694508.000405745399819No Hit
AACATTCAACGCTGTCGGTGAGT3109584.368759627321165No Hit
AACATTCAACGCTGTCGGTGAG1834112.5768064240399093No Hit
CCCCCACTGCTAAATTTGACTG1813872.5483705275982738No Hit
TTCAAGTAATCCAGGATAGGCT1776942.496486256077049No Hit
TAGCAGCACGTAAATATTGGCG1704712.3950077580543554No Hit
AAGCTGCCAGTTGAAGAACTGT1684552.3666842564603154No Hit
TATTGCACTTGTCCCGGCCTGTAA1241041.7435812707473863No Hit
TATTGCACTTGTCCCGGCCTGTAT1178821.6561661780300667No Hit
TATTGCACTTGTCCCGGCCTGT919531.2918804276174372No Hit
TCGTACGACTCTTAGCGG786571.105080190913888No Hit
TAGCTTATCAGACTGATGTTGAC734281.0316161086543472No Hit
TAGCTTATCAGACTGATGTTGACT679330.954414897712259No Hit
TATTGCACTTGTCCCGGCCTGTA600120.8431299492368671No Hit
TAGCTTATCAGACTGATGTTGA596520.8380721810950743No Hit
TGAGGTAGTAGGTTGTATAGTT538510.7565718672324623No Hit
TGTAAACATCCTTGACTGGAAGCT413610.5810954114241494No Hit
ACTGGACTTGGAGTCAGAAGGC396920.5576470363445599No Hit
CATAAAGTAGAAAGCACTACT388900.5463794528731214No Hit
AGCAGCATTGTACAGGGCTATGA387080.5438224700903261No Hit
TTCAAGTAATTCAGGATAGGTT340110.4778326451958789No Hit
CAACGGAATCCCAAAAGCAGCTGT321210.4512793624514665No Hit
TGTAAACATCCCCGACTGGAAGCT310720.4365415880605202No Hit
TGAGGTAGTAGATTGTATAGTT310220.43583912026304894No Hit
CTCCCACTGCTTCACTTGACTAG283790.3987066724887198No Hit
TTCTCACTACTGCACTTGACTAG276600.3886051855610835No Hit
CCCATAAAGTAGAAAGCACTAC273880.38478376074284004No Hit
CAACGGAATCCCAAAAGCAGCT264910.3721814884562062No Hit
CTTCTCACTACTGCACTTGACTAG254530.3575982569807035No Hit
CGCCTGTCTGAGCGTCGCT247560.3478058558839546No Hit
CACCCGGCTGTGTGCACATGTGC237570.3337705492904794No Hit
CATTGCACTTGTCTCGGTCTGA230730.32416078982107305No Hit
ACCCCCCACTGCTAAATTTGACTG215730.3030867558969362No Hit
AACATTCAACGCTGTCGGTGAGTT203360.28570770258749795No Hit
CCCCCACTGCTAAATTTGACTGGCTT192420.27033770717882755No Hit
CCCCCCACTGCTAAATTTGACTGG181650.2552065508212973No Hit
CCCATAAAGTAGAAAGCACT167260.23498952761007527No Hit
ACCGGGTGCTGTAGGCTT163300.22942598265410316No Hit
CCCCCCACTGCTAAATTTGACTGGCT156630.220055062235837No Hit
TATTGCACTTGTCCCGGCCTGTT155560.21855178114924856No Hit
CAACACCAGTCGATGGGCTGT147230.2068486676433779No Hit
CTCCCACTGCTTCACTTGACTAGCCT147100.2066660260160354No Hit
TATTGCACTTGTCCCGGCCTGTAG146630.2060057062864124No Hit
CGCGACCTCAGATCAGACGTGGCG145470.20437598099627918No Hit
TGAGGTAGTAGTTTGTACAGTT138610.1947381228149739No Hit
TCGTTTCCCGGCCAATGCACCA132960.18680023670354903No Hit
CACTAGATTGTGAGCTCCTGGA129560.18202345568074468No Hit
AACGGAATCCCAAAAGCAGCTG123430.17341120048374742No Hit
CTACGCCTGTCTGAGCGTCGCT114180.1604155462305297No Hit
TGTAAACATCCTACACTCTCAGCT113770.1598395226366033No Hit
TGTCAGTTTGTCAAATACCCCAA110580.15535777808873685No Hit
TACCACCCTGAACGCGCCCGA109830.15430407639253No Hit
CACTAGATTGTGAGCTCCTGGAA107620.1511991687277072No Hit
CGCGACCTCAGATCAGACGTGG107020.1503562073707417No Hit
ACTGGACTTGGAGTCAGAAGGCA106650.14983638120061302No Hit
TAGCAGCACGTAAATATTGGC106350.14941490052213027No Hit
ATCCCGGACGAGCCCCCA106160.1491479627590912No Hit
ACCGGGTGCTGTAGGCTTA102350.14379515814236044No Hit
TGAGGTAGTAGTTTGTGCTGTT102350.14379515814236044No Hit
CAAAGAATTCTCCTTTTGGGCT97450.1369109737271424No Hit
TAGCTTATCAGACTGATGTTGACA97080.13639114755701368No Hit
ACCGGGTGCTGTAGGCTTT96600.1357167784714413No Hit
AGCTCGGTCTGAGGCCCCTCAGT95820.1346209287073862No Hit
TTCAAGTAATCCAGGATAGGCTT94820.13321599311244375No Hit
CCCCCCACTGCTAAATTTGACTGTA93260.1310242935843335No Hit
ACCACAGGGTAGAACCACGGAC93020.13068710904154732No Hit
AACATTCAACGCTGTCGGTGA91170.12808797819090376No Hit
TGATGTAGTAGGTTGTATAGTT90390.12699212842684865No Hit
TGTAAACATCCCCGACTGGAAGC90370.1269640297149498No Hit
ACTGGACTTGGAGTCAGAAGGCT90250.1267954374435567No Hit
CCCCCCACTGCTAAATTTGACTGT90210.126739240019759No Hit
TATTGCACTTGTCCCGGCCTGTAAT89770.12612106835798434No Hit
TGAGGTAGTAGGTTGTGTGGTT89240.12537645249266482No Hit
TATTGCACTTGTCCCGGCCTGTTT87070.1223277422516397No Hit
TATTGCACTTGTCCCGGCCTGA86060.12090875730074782No Hit
AACATTCAACGCTGTCGGTGAGA85750.12047322726631567No Hit
TACGCCTGTCTGAGCGTCGCT85230.11974266075694558No Hit
TTCACAGTGGCTAAGTTCTGC83180.11686254278731355No Hit
CGCGACCTCAGATCAGACGTGGC83020.11663775309212275No Hit
ACTTGACTTGGAGTCAGAAGGC82590.1160336307862975No Hit
CAAAGAATTCTCCTTTTGGGCTT80240.11273203213818273No Hit
TTCAAGTAATCCAGGATAGGC79930.11229650210375056No Hit
CAAAGTGCTGTTCGTGCAGGTAG78980.11096181328855523No Hit
ATCCCACCGCTGCCACCA77840.10936018671032083No Hit
TCGTTTCCCGGGCGGCGCACCA77200.10846102792955764No Hit
AGCAGCATTGTACAGGGCTATGT77120.10834863308196226No Hit
CAACGGAATCCCAAAAGCAGCTGA76770.1078569056237324No Hit
CAAAGTGCTGTTCGTGCAGGT76230.10709824040246348No Hit
TTCACAGTGGCTAAGTTCCGC75680.10632552582524513No Hit
ACTGGACTTGGAGTCAGAAGG74140.10416192500903375No Hit
GTGCGGTAACGCGACCGATCCCGGA73090.10268674263434416No Hit
TGTCAGTTTGTCAAATACCCCA72140.10135205381914883No Hit
ACGCCTGTCTGAGCGTCGCT71410.10032645083484083No Hit
TATTGCACTTGTCCCGGCCTGTAAA71400.1003124014788914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)