FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12335618
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG11474889.302233580838836No Hit
CAACGGAATCCCAAAAGCAGCTG9736207.892754136841786No Hit
AACATTCAACGCTGTCGGTGAGT5036354.082770721337188No Hit
AACATTCAACGCTGTCGGTGAG3344512.7112626217835216No Hit
CCCCCACTGCTAAATTTGACTG3049952.4724744232514335No Hit
TAGCAGCACGTAAATATTGGCG2929182.374570937589021No Hit
TATTGCACTTGTCCCGGCCTGTAA2740522.2216317009816615No Hit
TATTGCACTTGTCCCGGCCTGTAT2693132.1832144931855058No Hit
AAGCTGCCAGTTGAAGAACTGT2646902.1457376517333793No Hit
TTCAAGTAATCCAGGATAGGCT2409391.9531976427934132No Hit
TATTGCACTTGTCCCGGCCTGT1509901.224016502456545No Hit
TAGCTTATCAGACTGATGTTGACT1484261.2032311636109354No Hit
TAGCTTATCAGACTGATGTTGAC1367781.1088054121001478No Hit
TATTGCACTTGTCCCGGCCTGTA1225700.9936267481694067No Hit
TAGCTTATCAGACTGATGTTGA969560.7859841314800766No Hit
TGAGGTAGTAGGTTGTATAGTT820180.6648876448670833No Hit
TGTAAACATCCTTGACTGGAAGCT798560.6473611617999195No Hit
ACTGGACTTGGAGTCAGAAGGC745770.6045663865401798No Hit
AGCAGCATTGTACAGGGCTATGA700270.5676813273562784No Hit
TGTAAACATCCCCGACTGGAAGCT629630.5104162596474696No Hit
GCCTGTCTGAGCGTCGCT602400.4883419703820271No Hit
TGAGGTAGTAGATTGTATAGTT514680.4172308189180307No Hit
CATAAAGTAGAAAGCACTACT513620.416371518638142No Hit
TTCAAGTAATTCAGGATAGGTT506880.41090766591507616No Hit
CAACGGAATCCCAAAAGCAGCTGT492030.39886935539022045No Hit
TTAATGCTAATCGTGATAGGGGTT483280.3917760747779317No Hit
CCCCCACTGCTAAATTTGACTGGCTT483090.3916220492560648No Hit
TTCTCACTACTGCACTTGACTAG441080.35756619571066484No Hit
CTCCCACTGCTTCACTTGACTAG406050.3291687534422677No Hit
CCCATAAAGTAGAAAGCACTAC393130.31869501795532257No Hit
ACCCCCCACTGCTAAATTTGACTG381250.309064369535438No Hit
TATTGCACTTGTCCCGGCCTGTAG375700.3045652029756434No Hit
CGCCTGTCTGAGCGTCGCT374030.303211399704498No Hit
TCGTACGACTCTTAGCGG373250.30257908440420256No Hit
CATTGCACTTGTCTCGGTCTGA350850.28442028603674335No Hit
CTTCTCACTACTGCACTTGACTAG349160.28305026955276985No Hit
AACATTCAACGCTGTCGGTGAGTT346520.2809101254594622No Hit
CACCCGGCTGTGTGCACATGTGC323670.262386529803371No Hit
CCCCCCACTGCTAAATTTGACTGGCT320590.25988969502784537No Hit
CCCCCCACTGCTAAATTTGACTGG316620.2566713722814698No Hit
TATTGCACTTGTCCCGGCCTGTT289910.23501862654955755No Hit
CGCGACCTCAGATCAGACGTGGCG288410.2338026355874509No Hit
CACTAGATTGTGAGCTCCTGGA288240.23366482327841215No Hit
TCCTGTCTGAGCGTCGCT286220.23202728878277523No Hit
CACTAGATTGTGAGCTCCTGGAA285300.23128148099268317No Hit
GGAATACCGGGTGCTGTAGGCTTT284520.23064916569238766No Hit
CTCCCACTGCTTCACTTGACTAGCCT250950.20343528796044108No Hit
ATCCCGGACGAGCCCCCA238790.19357765456096324No Hit
CCCATAAAGTAGAAAGCACT228560.1852845961993959No Hit
AGCTCGGTCTGAGGCCCCTCAGT227820.18468470732475664No Hit
GGAATACCGGGTGCTGTAGGCTT227640.18453878840930385No Hit
TATTGCACTTGTCCCGGCCTGTAAT223950.18154745064252154No Hit
TGAGGTAGTAGTTTGTACAGTT217830.17658620751712642No Hit
ACTGGACTTGGAGTCAGAAGGCA217470.17629436968622084No Hit
TGTCAGTTTGTCAAATACCCCAA216690.1756620543859254No Hit
ACCGGGTGCTGTAGGCTT213370.17297066105646267No Hit
TATTGCACTTGTCCCGGCCTGTTT211830.17172224366869987No Hit
ACCGGGTGCTGTAGGCTTA205900.1669150260651716No Hit
TAGCTTATCAGACTGATGTTGACA203710.16513967926049591No Hit
CAAAGAATTCTCCTTTTGGGCT200480.16252124538875962No Hit
CAACACCAGTCGATGGGCTGT199830.16199431597184674No Hit
CCCCCCACTGCTAAATTTGACTGTA198910.16124850818175465No Hit
TATTGCACTTGTCCCGGCCTGTAAA190400.154349786123403No Hit
AACGGAATCCCAAAAGCAGCTG190400.154349786123403No Hit
CCCCCCACTGCTAAATTTGACTGT188260.1526149723507975No Hit
TGAGAACTGAATTCCATGGGTT180610.14641341844405364No Hit
TGTAAACATCCTACACTCTCAGCT180220.14609726079390592No Hit
ACTGGACTTGGAGTCAGAAGGCT175630.1423763284498596No Hit
TAGCAGCACGTAAATATTGGC173700.14081175341194904No Hit
TCGTTTCCCGGGCGGCGCACCA171360.1389148075110627No Hit
CTACGCCTGTCTGAGCGTCGCT171070.13867971592505537No Hit
TGTAAACATCCCCGACTGGAAGC168010.13619909436235786No Hit
CGCGACCTCAGATCAGACGTGG167100.13546139317867983No Hit
CAAAGAATTCTCCTTTTGGGCTT164860.1336455133419339No Hit
TGAGGTAGTAGTTTGTGCTGTT159990.12969759601829434No Hit
TTCAAGTAATCCAGGATAGGCTT157890.12799520867134503No Hit
ACTTGACTTGGAGTCAGAAGGC157180.1274196396159479No Hit
CAAAGTGCTGTTCGTGCAGGTAG151350.1226934880765601No Hit
ACTGGACTTGGAGTCAGAAGG143600.11641086810567577No Hit
TACGCCTGTCTGAGCGTCGCT143150.11604607081704378No Hit
TACCACCCTGAACGCGCCCGA140410.11382486065959566No Hit
ACCGGGTGCTGTAGGCTTT138610.11236567150506768No Hit
ACCACAGGGTAGAACCACGGAC137460.1114334117674526No Hit
AACATTCAACGCTGTCGGTGAGA135580.10990936976161227No Hit
AGCAGCATTGTACAGGGCTATGT135170.10957699889863645No Hit
TGTCAGTTTGTCAAATACCCCA134490.10902574966248145No Hit
TTCACCACCTTCTCCACCCAGC134330.10889604395985673No Hit
TGATGTAGTAGGTTGTATAGTT133950.10858799291612305No Hit
AACCGGGCGGAAACACCA132310.1072585094642198No Hit
CAACGGAATCCCAAAAGCAGCT132140.10712069715518105No Hit
TGAATACCGGGTGCTGTAGGCTTT130430.10573446745837946No Hit
TATTGCACTTGTCCCGGCCTGA127110.10304307412891678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)