FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6588988
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4843847.351417243437081No Hit
CAACGGAATCCCAAAAGCAGCTG3941415.981813899190589No Hit
AACATTCAACGCTGTCGGTGAGT2996934.548391953362185No Hit
AAGCTGCCAGTTGAAGAACTGT1843522.797880342170907No Hit
TAGCAGCACGTAAATATTGGCG1800522.7326199410288803No Hit
TTCAAGTAATCCAGGATAGGCT1513212.296574223537818No Hit
CCCCCACTGCTAAATTTGACTG1461872.218656339941733No Hit
AACATTCAACGCTGTCGGTGAG1283501.9479470899021214No Hit
TAGCTTATCAGACTGATGTTGAC969371.4711970943034045No Hit
TATTGCACTTGTCCCGGCCTGTAA961601.4594046915854149No Hit
TATTGCACTTGTCCCGGCCTGTAT857031.300700502110491No Hit
TATTGCACTTGTCCCGGCCTGT787181.1946902923483849No Hit
TAGCTTATCAGACTGATGTTGACT757741.150009682822309No Hit
TAGCTTATCAGACTGATGTTGA678641.0299608983959299No Hit
CGCCTGTCTGAGCGTCGCT536820.8147229893270408No Hit
TGTAAACATCCTTGACTGGAAGCT509390.7730929241334178No Hit
AGCAGCATTGTACAGGGCTATGA496060.7528621997793894No Hit
TGAGGTAGTAGGTTGTATAGTT488350.7411608580862493No Hit
TATTGCACTTGTCCCGGCCTGTA484160.7348017631842705No Hit
CGCGACCTCAGATCAGAC443370.6728954431241945No Hit
ACTGGACTTGGAGTCAGAAGGC417820.6341186233758508No Hit
TTAATGCTAATCGTGATAGGGGTT408330.6197158046121802No Hit
CATAAAGTAGAAAGCACTACT402450.610791824176945No Hit
TGTAAACATCCCCGACTGGAAGCT345040.5236616002336019No Hit
TCGTACGACTCTTAGCGG290280.4405532382211047No Hit
TTCAAGTAATTCAGGATAGGTT285470.43325317939568264No Hit
TGAGGTAGTAGATTGTATAGTT266160.40394670623167017No Hit
CAACGGAATCCCAAAAGCAGCTGT247980.3763552157023203No Hit
CCCATAAAGTAGAAAGCACTAC241930.3671732290300119No Hit
AACATTCAACGCTGTCGGTGAGTT240950.36568589895747267No Hit
CATTGCACTTGTCTCGGTCTGA209110.3173628484374232No Hit
CTACGCCTGTCTGAGCGTCGCT194360.29497701316196057No Hit
TGTAAACATCCTACACTCTCAGCT190330.2888607476595799No Hit
TTCTCACTACTGCACTTGACTAG189480.2875707164742143No Hit
TACGCCTGTCTGAGCGTCGCT180170.2734410807850917No Hit
CCCCCACTGCTAAATTTGACTGGCTT177310.26910050526727325No Hit
CTTCTCACTACTGCACTTGACTAG175510.266368674521793No Hit
ACCGGGTGCTGTAGGCTT155550.23607570692191276No Hit
CCCATAAAGTAGAAAGCACT144310.21901694160013646No Hit
CTCCCACTGCTTCACTTGACTAG143900.21839469126366598No Hit
CCCCCCACTGCTAAATTTGACTGG140180.21274890772300692No Hit
ACGCCTGTCTGAGCGTCGCT135960.20634428230860338No Hit
CACCCGGCTGTGTGCACATGTGC134620.20431058608696814No Hit
CCCCCCACTGCTAAATTTGACTGGCT129610.1967069905120483No Hit
TATTGCACTTGTCCCGGCCTGTT124520.18898198023732932No Hit
CGCGACCTCAGATCAGACGTGGCG124040.1882534920385346No Hit
CACTAGATTGTGAGCTCCTGGA122170.1854154234307302No Hit
CAACGGAATCCCAAAAGCAGCT117960.1790259748538015No Hit
TGAGGTAGTAGTTTGTACAGTT117840.17884385280410284No Hit
GCGTTCGCGCTTTCCCCTG115260.17492822873558125No Hit
TGTCAGTTTGTCAAATACCCCAA115240.17489787506063148No Hit
ACTGGACTTGGAGTCAGAAGGCA114800.1742300942117363No Hit
TAGCTTATCAGACTGATGTTGACA113430.17215086747767638No Hit
TATTGCACTTGTCCCGGCCTGTAG112840.17125543406665789No Hit
TGATGTAGTAGGTTGTATAGTT108970.16538199796387548No Hit
ACCCCCCACTGCTAAATTTGACTG108840.1651846990767019No Hit
TGAGGTAGTAGTTTGTGCTGTT105150.15958444604846753No Hit
CAACACCAGTCGATGGGCTGT104760.15899254938694682No Hit
TGTAAACATCCCCGACTGGAAGC102930.15621518812904198No Hit
CAAAGAATTCTCCTTTTGGGCTT102640.15577505984227016No Hit
ACTTGACTTGGAGTCAGAAGGC102370.15536528523044812No Hit
ATCCCGGACGAGCCCCCA102170.15506174848095033No Hit
TTCAAGTAATCCAGGATAGGCTT101030.15333158900881289No Hit
CAAAGAATTCTCCTTTTGGGCT100640.15273969234729218No Hit
CACTAGATTGTGAGCTCCTGGAA98900.15009892262666133No Hit
ACCACAGGGTAGAACCACGGAC96550.1465323658200622No Hit
GGGCTACGCCTGTCTGAGCGTCGCT96050.1457735239463177No Hit
TCGTTTCCCGGCCAATGCACCA95360.14472632216055029No Hit
AGCAGCATTGTACAGGGCTATGT93520.14193378406517054No Hit
CTCCCACTGCTTCACTTGACTAGCCT92890.1409776433042525No Hit
AGCTCGGTCTGAGGCCCCTCAGT92210.13994561835595998No Hit
AACATTCAACGCTGTCGGTGAGA90440.13725931812290446No Hit
TAGCAGCACGTAAATATTGGC85560.12985302143515817No Hit
CAAAGTGCTGTTCGTGCAGGTAG84310.12795591675079693No Hit
ACCGGGTGCTGTAGGCTTT83750.1271060138522031No Hit
TTATAAAGCAATGAGACTGATT83690.12701495282735376No Hit
AACGGAATCCCAAAAGCAGCTG82310.12492054925581896No Hit
AACATTCAACGCTGTCGGTGA80640.12238601739751234No Hit
CCAAGTCCTTCTGATCGAGGCCC80200.12171823654861717No Hit
ACTGGACTTGGAGTCAGAAGG78690.11942653408990879No Hit
TGTCAGTTTGTCAAATACCCCA78420.11901675947808678No Hit
CGCTACCTCAGATCAGAC78100.11853110067889029No Hit
CCCCCCACTGCTAAATTTGACTGTA76950.11678576436927794No Hit
AGCAGCATTGTACAGGGCTATG76390.1159358614706841No Hit
TGTAAACATCCTACACTCAGCT75510.11460029977289381No Hit
TAGCAGCACGTAAATATTGGCGT74490.11305226235045504No Hit
CGCGACCTCAGATCAGACGTGG74420.1129460244881308No Hit
GCCTGTCTGAGCGTCGCT73680.11182293851498895No Hit
TTCAAGTAATCCAGGATAGGC73650.11177740800256428No Hit
CCTCGACACAAGGGTTTGT73240.11115515766609378No Hit
ACTGGACTTGGAGTCAGAAGGCT72790.11047219997972375No Hit
CAAAGTGCTGTTCGTGCAGGT72520.11006242536790171No Hit
TGAGGTAGTAGGTTGTGTGGTT72360.10981959596830349No Hit
TATTGCACTTGTCCCGGCCTGTAAT72000.10927322981920745No Hit
CGCGACCTCAGATCAGACGTGGC70860.10754307034706999No Hit
GTGCGGTAACGCGACCGATCCCGGA70810.10746718615969554No Hit
CCCGCCACTGCTAAATTTGACTG67190.10197317099378538No Hit
TTCACAGTGGCTAAGTTCCG67010.10169998791923736No Hit
TACCTGGTTGATCCTGCC66410.10078937767074399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)