FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965265
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5805708.33521768374929No Hit
CCCCCCACTGCTAAATTTGACTG4874826.998757405497134No Hit
AACATTCAACGCTGTCGGTGAGT2467993.5432822728209192No Hit
TTCAAGTAATCCAGGATAGGCT2101223.0167122141081495No Hit
TAGCAGCACGTAAATATTGGCG1907712.73889076725724No Hit
AACATTCAACGCTGTCGGTGAG1877832.6959921840733982No Hit
AAGCTGCCAGTTGAAGAACTGT1623482.3308230196553903No Hit
CCCCCACTGCTAAATTTGACTG1548202.2227438582738777No Hit
TATTGCACTTGTCCCGGCCTGTAA1073621.5413914617749647No Hit
TATTGCACTTGTCCCGGCCTGTAT1006241.444654295278069No Hit
TATTGCACTTGTCCCGGCCTGT870151.2492704871961082No Hit
TGAGGTAGTAGGTTGTATAGTT747301.0728952882625427No Hit
TAGCTTATCAGACTGATGTTGAC722251.0369311146094227No Hit
TAGCTTATCAGACTGATGTTGA667910.9589154181499197No Hit
TATTGCACTTGTCCCGGCCTGTA634840.9114369661455809No Hit
TAGCTTATCAGACTGATGTTGACT594310.853248225300832No Hit
TGAGGTAGTAGATTGTATAGTT453180.6506285116215966No Hit
TGTAAACATCCTTGACTGGAAGCT419390.6021163588176474No Hit
CATAAAGTAGAAAGCACTACT418440.6007524480403832No Hit
AGCAGCATTGTACAGGGCTATGA383360.5503882479704649No Hit
CAACGGAATCCCAAAAGCAGCT366860.5266992713127211No Hit
TTCAAGTAATTCAGGATAGGTT365370.524560084935749No Hit
ACTGGACTTGGAGTCAGAAGGC339910.4880072761050729No Hit
GCCTGTCTGAGCGTCGCT336140.4825947038626671No Hit
TACGCCTGTCTGAGCGTCGCT327320.4699318690674368No Hit
CGCCTGTCTGAGCGTCGCT306810.44048575323408373No Hit
TTCTCACTACTGCACTTGACTAG286840.41181491300043865No Hit
CATTGCACTTGTCTCGGTCTGA271420.38967648754211076No Hit
ACCGGGTGCTGTAGGCTT265470.38113409898977285No Hit
CAACGGAATCCCAAAAGCAGCTGT260890.37455861334780516No Hit
CTACGCCTGTCTGAGCGTCGCT254620.36555680221786246No Hit
CTCCCACTGCTTCACTTGACTAG254560.3654706604845616No Hit
TCGTACGACTCTTAGCGG251450.3610056473084656No Hit
TGTAAACATCCCCGACTGGAAGCT249190.35776097535413226No Hit
TGAGGTAGTAGTTTGTACAGTT207420.29779197202116503No Hit
CCCATAAAGTAGAAAGCACTAC206710.29677262817710454No Hit
CTTCTCACTACTGCACTTGACTAG198810.2854306332924878No Hit
CACCCGGCTGTGTGCACATGTGC195730.28100869098304226No Hit
CCCCCACTGCTAAATTTGACTGGCTT188440.2705424703869846No Hit
TCGTTTCCCGGCCAATGCACCA173890.24965310006151956No Hit
CAACACCAGTCGATGGGCTGT166460.23898588208775975No Hit
CCCATAAAGTAGAAAGCACT165170.2371338348217907No Hit
ATCCCGGACGAGCCCCCA162300.23301338857889828No Hit
AACATTCAACGCTGTCGGTGAGTT156560.22477249609311345No Hit
ACCGGGTGCTGTAGGCTTA151700.21779501569574164No Hit
AACGGAATCCCAAAAGCAGCTG150090.21548354585216783No Hit
TCCTGTCTGAGCGTCGCT147150.21126260092042443No Hit
TATTGCACTTGTCCCGGCCTGTT146970.21100417572052177No Hit
CGTTCGCGCTTTCCCCTG146550.21040118358741558No Hit
AGCTCGGTCTGAGGCCCCTCAGT142910.20517525176716178No Hit
TGAGGTAGTAGTTTGTGCTGTT142580.2047014722340069No Hit
TTCACAGTGGCTAAGTTCCG141370.202964280612439No Hit
ACCGGGTGCTGTAGGCTTT133420.19155050095007153No Hit
TATTGCACTTGTCCCGGCCTGTAG129530.18596564524106404No Hit
CTCCCACTGCTTCACTTGACTAGCCT128630.18467351924155076No Hit
TTCACAGTGGCTAAGTTCTGC125990.18088328297631173No Hit
ACCCCCCACTGCTAAATTTGACTG123960.17796882099963174No Hit
TGTAAACATCCTACACTCTCAGCT122300.17558556637830722No Hit
TTCACAGTGGCTAAGTTCCGC117160.16820609122553126No Hit
TATTGCACTTGTCCCGGCCTGA113930.16356879458283355No Hit
CAACGGAATCCCAAAAGCAGCTGA112590.16164496253911373No Hit
TAGCAGCACGTAAATATTGGC109970.15788344018497502No Hit
CCCCCCACTGCTAAATTTGACTGG109890.15776858454057383No Hit
TGAGGTAGTAGGTTGTGTGGTT106470.1528585057424233No Hit
CACTAGATTGTGAGCTCCTGGA102110.14659887312255887No Hit
TTCAAGTAATCCAGGATAGGC99030.14217693081311336No Hit
GTCAGGATGGCCGAGCGGTCTAAGG98460.1413585843467549No Hit
ACTGGACTTGGAGTCAGAAGGCA97170.13950653708078586No Hit
TAGCTTATCAGACTGATGTTGACA96580.13865947670332715No Hit
CGCGACCTCAGATCAGACGTGGCG96530.1385876919255764No Hit
CAAAGAATTCTCCTTTTGGGCT96000.13782677328141857No Hit
ACTGGACTTGGAGTCAGAAGGCT89780.12889674692922667No Hit
AATGCACCCGGGCAAGGATTCT88790.12747540832976204No Hit
ACTGGACTTGGAGTCAGAAGG88720.127374909640911No Hit
CCCCCCACTGCTAAATTTGACTGGCT86150.12368517206452304No Hit
TAGCAGCACATCATGGTTTACA85310.12247918779831062No Hit
CACTAGATTGTGAGCTCCTGGAA83680.12013900404363653No Hit
AGCAGCATTGTACAGGGCTATGT83200.11944987017722944No Hit
TTCACAGTGGCTAAGTTCTG80970.11624826908954648No Hit
TGTAAACATCCCCGACTGGAAGC80710.11587498824524264No Hit
TTCAAGTAATCCAGGATAGGCTT80640.11577448955639161No Hit
AACATTCAACGCTGTCGGTGAGA78920.11330509320176618No Hit
ACGCCTGTCTGAGCGTCGCT78050.11205603806890332No Hit
TATTGCACTTGTCCCGGCCTGTTT77220.11086441075824106No Hit
TGATGTAGTAGGTTGTATAGTT76970.11050548686948737No Hit
CAAAGTGCTGTTCGTGCAGGT76920.11043370209173663No Hit
CGCGACCTCAGATCAGACGTGG76550.10990249473638117No Hit
GTGCGGTAACGCGACCGATCCCGGA75920.10899800653672186No Hit
TGAGGTAGTAGGTTGTATAGT75610.10855294091466727No Hit
TGTCAGTTTGTCAAATACCCCA74200.10652861018209646No Hit
ACCACAGGGTAGAACCACGGAC73650.10573897762683833No Hit
AGCAGCATTGTACAGGGCTATG71120.10210666787265094No Hit
ATCACATTGCCAGGGATTTCC71000.10193438440604917No Hit
CACCCGTAGAACCGACCTTGCG70130.10068532927318631No Hit
GACCCGCGGGCGCTCTCCAGTCCT70040.10055611667323498No Hit
AATGACACGATCACTCCCGTTG70010.10051304580658453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)