FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6863368
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6403969.330637669435763No Hit
CAACGGAATCCCAAAAGCAGCTG3853705.614881789815146No Hit
AAGCTGCCAGTTGAAGAACTGT2129913.1033014694826213No Hit
TTCAAGTAATCCAGGATAGGCT2009312.9275859898522127No Hit
AACATTCAACGCTGTCGGTGAGT2006402.9233460889755585No Hit
CCCCCACTGCTAAATTTGACTG1889932.753648063166655No Hit
TAGCAGCACGTAAATATTGGCG1817262.6477671020991442No Hit
AACATTCAACGCTGTCGGTGAG1121041.633367174833114No Hit
TAGCTTATCAGACTGATGTTGAC950211.384466052235579No Hit
GCCTGTCTGAGCGTCGCT849611.2378907848158514No Hit
TAGCTTATCAGACTGATGTTGACT749841.0925248362028672No Hit
TATTGCACTTGTCCCGGCCTGTAA743781.0836953519030306No Hit
TATTGCACTTGTCCCGGCCTGTAT678420.9884651383985239No Hit
TGTAAACATCCTTGACTGGAAGCT666350.9708790203293776No Hit
TGAGGTAGTAGGTTGTATAGTT633240.9226373990145945No Hit
TAGCTTATCAGACTGATGTTGA598070.8713943358421115No Hit
TATTGCACTTGTCCCGGCCTGT525190.7652074025463883No Hit
CGCCTGTCTGAGCGTCGCT490500.7146637044669615No Hit
TGAGGTAGTAGATTGTATAGTT454220.6618033595167853No Hit
CATAAAGTAGAAAGCACTACT447040.6513420233331507No Hit
AGCAGCATTGTACAGGGCTATGA415670.605635600480697No Hit
TATTGCACTTGTCCCGGCCTGTA351990.5128531648018874No Hit
TGTAAACATCCCCGACTGGAAGCT326020.47501459924631756No Hit
TTCTCACTACTGCACTTGACTAG322160.4693905382896561No Hit
TTCAAGTAATTCAGGATAGGTT320290.46666592844795735No Hit
AACCGGGCGGAAACACCA303800.44263982348025055No Hit
ACTGGACTTGGAGTCAGAAGGC274320.3996871506817061No Hit
CCCATAAAGTAGAAAGCACTAC272570.3971373821132715No Hit
CTCCCACTGCTTCACTTGACTAG258620.3768120840963212No Hit
CAACGGAATCCCAAAAGCAGCTGT255390.3721059398242962No Hit
CTTCTCACTACTGCACTTGACTAG252000.3671666738545857No Hit
TGTAAACATCCTACACTCTCAGCT221450.32265499970276984No Hit
CCCATAAAGTAGAAAGCACT213310.3107949333330225No Hit
CTACGCCTGTCTGAGCGTCGCT212010.30890081953932824No Hit
CCCCCACTGCTAAATTTGACTGGCTT202940.29568573330178416No Hit
CCCCCCACTGCTAAATTTGACTGG198480.28918746597880224No Hit
ACCCCCCACTGCTAAATTTGACTG195810.2852972476486763No Hit
TGAGGTAGTAGTTTGTACAGTT190270.2772254088663175No Hit
CATTGCACTTGTCTCGGTCTGA185870.27081456217996763No Hit
TACGCCTGTCTGAGCGTCGCT179580.26164996543970837No Hit
CCCCCCACTGCTAAATTTGACTGGCT172860.2518588541369194No Hit
TGTCAGTTTGTCAAATACCCCAA172370.2511449189377577No Hit
AACATTCAACGCTGTCGGTGAGTT172300.2510429281950203No Hit
ACCGGGTGCTGTAGGCTT171090.24927994535627407No Hit
GACCCGCGGGCGCTCTCCAGTCCT157600.22962487222016945No Hit
CACCCGGCTGTGTGCACATGTGC156980.22872152564163833No Hit
ACGCCTGTCTGAGCGTCGCT145970.2126798388196582No Hit
TCGTACGACTCTTAGCGG141310.20589016937456947No Hit
CTCCCACTGCTTCACTTGACTAGCCT140030.20402519579308587No Hit
TAGCTTATCAGACTGATGTTGACA139300.20296157804739595No Hit
GGAATACCGGGTGCTGTAGGCTTT131330.19134920348143944No Hit
TGTCAGTTTGTCAAATACCCCA129110.18811463992605382No Hit
CAAAGAATTCTCCTTTTGGGCT128770.18761925631847223No Hit
GTGCGGTAACGCGACCGATCCCGGA123030.17925601541400665No Hit
ACCGGGTGCTGTAGGCTTT120650.17578833016093556No Hit
TGAGGTAGTAGTTTGTGCTGTT115580.16840128636552784No Hit
AGCTCGGTCTGAGGCCCCTCAGT114500.16682771490615103No Hit
GCGTTCGCGCTTTCCCCTG114380.16665287363288694No Hit
TAGCAGCACGTAAATATTGGC113520.16539984450782763No Hit
TTCAAGTAATCCAGGATAGGCTT112950.1645693484598232No Hit
CGCGACCTCAGATCAGACGTGGCG111320.16219442116465269No Hit
TGTAAACATCCTACACTCAGCT109470.15949895153516466No Hit
TGATGTAGTAGGTTGTATAGTT106520.15520077023408915No Hit
CAACACCAGTCGATGGGCTGT104780.15266557177175988No Hit
TAGCAGCACGTAAATATTGGCGT104200.15182050561765012No Hit
GGGCTACGCCTGTCTGAGCGTCGCT103630.15099000956964567No Hit
ACCGGGTGCTGTAGGCTTA101620.1480614182424722No Hit
GGAATACCGGGTGCTGTAGGCTT97290.14175256229885969No Hit
TGTAAACATCCCCGACTGGAAGC95370.13895510192663427No Hit
CCCCCCACTGCTAAATTTGACTGTA94440.1376000820588376No Hit
TATTGCACTTGTCCCGGCCTGTT93920.13684243654135986No Hit
TTCAAGTAATCCAGGATAGGC93270.13589537964451273No Hit
AGCAGCATTGTACAGGGCTATGT93130.13569139815903794No Hit
TTCACAGTGGCTAAGTTCCG91990.13403040606302913No Hit
CAACGGAATCCCAAAAGCAGCT91300.1330250687417606No Hit
CCCCCCACTGCTAAATTTGACTGT89250.13003819699016578No Hit
CAAAGAATTCTCCTTTTGGGCTT87310.12721159640572965No Hit
ATCCCACCGCTGCCACCA86090.1254340434608781No Hit
AACGGAATCCCAAAAGCAGCTG84040.12244717170928326No Hit
TATTGCACTTGTCCCGGCCTGTAG80920.11790129860441696No Hit
TTATAAAGCAATGAGACTGATT79370.11564293215808916No Hit
TGATGTAGTAGATTGTATAGTT77240.11253949955765158No Hit
TGAGGTAGTAGGTTGTGTGGTT75570.11010629183805969No Hit
TCCCACTGCTTCACTTGACTAG75500.11000430109532229No Hit
ACTGGACTTGGAGTCAGAAGGCA74900.10913009472900184No Hit
TTCACAGTGGCTAAGTTCCGC74020.10784792539173187No Hit
ATCACATTGCCAGGGATTTCCA71680.10443852056308214No Hit
AGCAGCATTGTACAGGGCTATG71630.10436567003255545No Hit
ACTGGACTTGGAGTCAGAAGGCT68970.10049002180853482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)