FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10266403
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG124732112.14954254182307No Hit
CAACGGAATCCCAAAAGCAGCTG8621488.397761124319784No Hit
CCCCCACTGCTAAATTTGACTG4187464.078799556183407No Hit
TTCAAGTAATCCAGGATAGGCT2510582.445432933034092No Hit
AACATTCAACGCTGTCGGTGAGT2456042.392308192070777No Hit
AACATTCAACGCTGTCGGTGAG1612021.57018967597512No Hit
TCGTACGACTCTTAGCGG1320931.2866531734629938No Hit
AAGCTGCCAGTTGAAGAACTGT1297851.2641720766270328No Hit
TAGCAGCACGTAAATATTGGCG1297401.263733753681791No Hit
GCCTGTCTGAGCGTCGCT1247511.2151383498193087No Hit
CGCCTGTCTGAGCGTCGCT846660.8246900107077425No Hit
ACCGGGTGCTGTAGGCTT744200.7248887463311152No Hit
ACCGGGTGCTGTAGGCTTA725520.7066934738486302No Hit
CATAAAGTAGAAAGCACTACT670530.6531304099400735No Hit
ATCCCGGACGAGCCCCCA645260.6285161414372687No Hit
ACTGGACTTGGAGTCAGAAGGC644390.6276687170764678No Hit
CCCATAAAGTAGAAAGCACT595690.5802324338914029No Hit
CACCCGGCTGTGTGCACATGTGC541030.5269908068093566No Hit
ACCGGGTGCTGTAGGCTTT540030.5260167558199303No Hit
CTCCCACTGCTTCACTTGACTAG535850.521945222684128No Hit
TATTGCACTTGTCCCGGCCTGT513680.5003505122485451No Hit
TATTGCACTTGTCCCGGCCTGTAA498120.4851942788530706No Hit
CAACGGAATCCCAAAAGCAGCTGT497330.4844247785714237No Hit
CCCATAAAGTAGAAAGCACTAC465650.45356684322639584No Hit
AGCAGCATTGTACAGGGCTATGA454970.44316397865932206No Hit
TAGCTTATCAGACTGATGTTGA447350.435741710119893No Hit
TATTGCACTTGTCCCGGCCTGTAT444100.43257604440425723No Hit
AACCGGGCGGAAACACCA420560.4096468841131602No Hit
CATTGCACTTGTCTCGGTCTGA389200.3791006450847488No Hit
TAGCTTATCAGACTGATGTTGAC380230.3703634077095941No Hit
TGAGGTAGTAGGTTGTATAGTT369230.35964884682590387No Hit
GACCCGCGGGCGCTCTCCAGTCCT361030.35166162871260753No Hit
CAACGGAATCCCAAAAGCAGCT335530.3268233284822347No Hit
CTACGCCTGTCTGAGCGTCGCT325140.3167029387020946No Hit
CAACACCAGTCGATGGGCTGT318540.31027420217188045No Hit
TTCAAGTAATTCAGGATAGGTT295330.28766647870729406No Hit
TACGCCTGTCTGAGCGTCGCT290480.2829423314085761No Hit
TATTGCACTTGTCCCGGCCTGTA280980.27368884700902546No Hit
CACTAGATTGTGAGCTCCTGGA279320.2720719223665777No Hit
TCCTGTCTGAGCGTCGCT278130.27091280168916027No Hit
AGCTCGGTCTGAGGCCCCTCAGT273350.2662568379597022No Hit
TAGCTTATCAGACTGATGTTGACT267450.2605099371220865No Hit
TTCTCACTACTGCACTTGACTAG261470.2546851122053167No Hit
TGAGGTAGTAGATTGTATAGTT252600.24604527992910466No Hit
CCCCCCACTGCTAAATTTGACTGG243000.23669439043061138No Hit
CTTCTCACTACTGCACTTGACTAG236410.2302753944102915No Hit
CACTAGATTGTGAGCTCCTGGAA226670.22078813777327852No Hit
CCCCCACTGCTAAATTTGACTGGCTT215230.20964499445424067No Hit
CGCGACCTCAGATCAGACGT213210.2076774114555994No Hit
ACCCCCCACTGCTAAATTTGACTG209610.20417082789366442No Hit
AACGGAATCCCAAAAGCAGCTG206230.20087853554940321No Hit
ACGCCTGTCTGAGCGTCGCT201200.19597905907258853No Hit
ACTGGACTTGGAGTCAGAAGGCT199670.19448876105876617No Hit
CCCCCCACTGCTAAATTTGACTGT198050.1929107984558954No Hit
CCCCCCACTGCTAAATTTGACTGGCT194040.1890048539882956No Hit
TGAGAACTGAATTCCATGGGTT193180.18816717013738893No Hit
TTAATGCTAATCGTGATAGGGGTT188770.18387160527401858No Hit
AACATTCAACGCTGTCGGTGAGTT179820.1751538489186524No Hit
CGCGACCTCAGATCAGACGTGGCG166530.16220871126917577No Hit
CGCGACCTCAGATCAGACGTGGC162920.15869238719734652No Hit
ACTGGACTTGGAGTCAGAAGG160790.15661765858986834No Hit
TCGTTTCCCGGCCAATGCACCA157000.15292600533994233No Hit
TGTAAACATCCTTGACTGGAAGCT156270.15221494811766106No Hit
TGTAAACATCCTACACTCTCAGCT154320.15031554868827962No Hit
TACCACCCTGAACGCGCCCGA147530.14370174247007447No Hit
TCGTTTCCCGGGCGGCGCACCA146320.14252314077286857No Hit
GGAATACCGGGTGCTGTAGGCTTT145840.14205559629794387No Hit
ACTGGACTTGGAGTCAGAAGGCA145460.14168545692196186No Hit
ATCCCACCGCTGCCACCA138110.13452618214967793No Hit
TTCAAGTAATCCAGGATAGGCTT136760.1332112133139523No Hit
TGTAAACATCCCCGACTGGAAGCT136690.13314302974469247No Hit
CCCCCCACTGCTAAATTTGACTGTA135670.13214949773547754No Hit
TTCACAGTGGCTAAGTTCCG132830.12938319292550662No Hit
ACCACAGGGTAGAACCACGGAC131660.12824355326787776No Hit
CAAAGAATTCTCCTTTTGGGCT129910.12653896403638157No Hit
CCCATAAAGTAGAAAGCACTA129000.12565257763600357No Hit
CGCGACCTCAGATCAGACGTGG121310.11816212552731467No Hit
TTCAAGTAATCCAGGATAGGC121110.11796731532942939No Hit
GGAATACCGGGTGCTGTAGGCTT116660.11363278842648199No Hit
CCCCCCACTGCTAAATTTGACTGA110590.10772029892066384No Hit
AGCAGCATTGTACAGGGCTATGT108150.10534361450646346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)