FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6409912
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG64310710.033008253467441No Hit
CAACGGAATCCCAAAAGCAGCTG2618304.084767466386434No Hit
CCCCCACTGCTAAATTTGACTG2055963.2074699309444497No Hit
AACATTCAACGCTGTCGGTGAGT1881912.935937342041513No Hit
AAGCTGCCAGTTGAAGAACTGT1595022.4883648948690715No Hit
CGCCTGTCTGAGCGTCGCT1448872.2603586445492545No Hit
TAGCAGCACGTAAATATTGGCG1403552.1896556458185388No Hit
TTCAAGTAATCCAGGATAGGCT1348782.1042098549870887No Hit
AACATTCAACGCTGTCGGTGAG1153461.7994942832288492No Hit
GCCTGTCTGAGCGTCGCT777911.2136048045589394No Hit
TAGCTTATCAGACTGATGTTGAC701941.0950852367395996No Hit
TATTGCACTTGTCCCGGCCTGT607110.9471424880715991No Hit
TATTGCACTTGTCCCGGCCTGTAA562110.8769387161633421No Hit
TATTGCACTTGTCCCGGCCTGTAT523500.8167038798660575No Hit
TGAGGTAGTAGGTTGTATAGTT496150.774035587384039No Hit
TAGCTTATCAGACTGATGTTGA486060.7582943416383875No Hit
TAGCTTATCAGACTGATGTTGACT481660.7514299728295801No Hit
ACCGGGTGCTGTAGGCTT439550.6857348431616535No Hit
TGTAAACATCCTTGACTGGAAGCT431420.6730513617035616No Hit
TATTGCACTTGTCCCGGCCTGTA414090.6460151090997817No Hit
TACGCCTGTCTGAGCGTCGCT382250.5963420402651394No Hit
AGCAGCATTGTACAGGGCTATGA380130.5930346625663504No Hit
GCGTTCGCGCTTTCCCCTG368420.5747660810320017No Hit
CATAAAGTAGAAAGCACTACT351940.5490558996753777No Hit
ACTGGACTTGGAGTCAGAAGGC333240.5198823322379466No Hit
TTAATGCTAATCGTGATAGGGGTT316520.4937977307644785No Hit
TGAGGTAGTAGATTGTATAGTT299410.46710469660113896No Hit
TCGTACGACTCTTAGCGG297790.46457736081244166No Hit
ACCGGGTGCTGTAGGCTTT274940.428929445521249No Hit
ACGCCTGTCTGAGCGTCGCT272160.4245924125011388No Hit
AACCGGGCGGAAACACCA239080.372984839729469No Hit
CTACGCCTGTCTGAGCGTCGCT236830.3694746511340561No Hit
TCCTGTCTGAGCGTCGCT229730.3583980560107533No Hit
TGTAAACATCCCCGACTGGAAGCT222190.3466350240065698No Hit
ACCGGGTGCTGTAGGCTTA215590.33633847079335877No Hit
TTCAAGTAATTCAGGATAGGTT207030.3229841532925881No Hit
CATTGCACTTGTCTCGGTCTGA196250.3061664497110101No Hit
CCCATAAAGTAGAAAGCACT190370.29699315684833116No Hit
AACATTCAACGCTGTCGGTGAGTT181760.28356083515655134No Hit
CAACACCAGTCGATGGGCTGT169810.2649178335053586No Hit
ATCCCGGACGAGCCCCCA166130.25917672504708333No Hit
CACCCGGCTGTGTGCACATGTGC148930.2323432833399273No Hit
TTCACAGTGGCTAAGTTCCG139210.21717926860774375No Hit
TTCTCACTACTGCACTTGACTAG133720.20861440843493637No Hit
CAACGGAATCCCAAAAGCAGCTGT133070.20760035395181714No Hit
CCCATAAAGTAGAAAGCACTAC130630.2037937494305694No Hit
CTCCCACTGCTTCACTTGACTAG121800.19001820929834917No Hit
CCTCGACACAAGGGTTTGT121160.18901975565343174No Hit
TGAGGTAGTAGTTTGTACAGTT121030.1888169447568079No Hit
TCGTTCGCGCTTTCCCCTG119960.18714765506921155No Hit
TACCTGGTTGATCCTGCC119250.18603999555688128No Hit
CAACGGAATCCCAAAAGCAGCT118740.1852443528085877No Hit
CTTCTCACTACTGCACTTGACTAG117230.18288862624011062No Hit
TGTCAGTTTGTCAAATACCCCAA117180.18281062204910145No Hit
TGTAAACATCCTACACTCTCAGCT111880.17454217780212897No Hit
ACTGGACTTGGAGTCAGAAGGCA107080.1670537754652482No Hit
TGAGGTAGTAGTTTGTGCTGTT101560.15844211277783535No Hit
CCCCCCACTGCTAAATTTGACTGG100970.15752166332392706No Hit
ACTGGACTTGGAGTCAGAAGG100530.15683522644304632No Hit
TATTGCACTTGTCCCGGCCTGTT99670.15549355435768852No Hit
CTGCGTTCGCGCTTTCCCCTG94620.1476151310657619No Hit
CCAAGTCCTTCTGATCGAGGCC92760.1447133751602206No Hit
CTCGACACAAGGGTTTGT92240.1439021315737252No Hit
TCGTTTCCCGGCCAATGCACCA91700.1430596863108261No Hit
AGCAGCATTGTACAGGGCTATG90860.14174921590187198No Hit
TGAAAAGAACTTTGAAGAGAG90750.14157760668165179No Hit
CGCGACCTCAGATCAGACGTGGCG89070.13895666586374353No Hit
TAGCAGCACGTAAATATTGGC86740.135321670562716No Hit
CACTAGATTGTGAGCTCCTGGA86340.1346976370346426No Hit
CCAAGTCCTTCTGATCGAGGCCC86030.13421401105038572No Hit
TTATAAAGCAATGAGACTGATT85450.13330916243467927No Hit
ACCACAGGGTAGAACCACGGAC84810.13231070878976187No Hit
AGCAGCATTGTACAGGGCTATGT83790.1307194232931747No Hit
ACCCCCCACTGCTAAATTTGACTG83520.13029820066172515No Hit
GACCCGCGGGCGCTCTCCAGTCCT81910.12778646571122973No Hit
AACGGAATCCCAAAAGCAGCTG80870.1261639785382389No Hit
TGAGGTAGTAGGTTGTGTGGTT80320.12530593243713797No Hit
ACTGGACTTGGAGTCAGAAGGCT79120.12343383185291779No Hit
TAGCAGCACGTAAATATTGGCGT78570.12257578575181688No Hit
CAAGTCCTTCTGATCGAGGCCC77800.12137452121027559No Hit
GTGCGGTAACGCGACCGATCCCGGA76320.11906559715640402No Hit
CAAGTCCTTCTGATCGAGGCC75010.11702188735196364No Hit
ATCCCACCGCTGCCACCA73800.11513418592954162No Hit
TAGCTTATCAGACTGATGTTGACA73100.11404212725541318No Hit
TTCAAGTAATCCAGGATAGGCTT73020.1139173205497985No Hit
CGCGACCTCAGATCAGACGTGG70210.1095334850150829No Hit
GGGCTACGCCTGTCTGAGCGTCGCT69770.10884704813420217No Hit
CACTAGATTGTGAGCTCCTGGAA68310.10656932575673426No Hit
TATTGCACTTGTCCCGGCCTGTAG67280.10496243942194525No Hit
TGTAAACATCCTACACTCAGCT66480.10371437236579847No Hit
CCCCCCACTGCTAAATTTGACTGT65560.10227909525122966No Hit
CCCCCACTGCTAAATTTGACTGGCTT65520.10221669189842232No Hit
CAAAGTGCTGTTCGTGCAGGT65370.10198267932539479No Hit
TTCAAGTAATCCAGGATAGGC64550.10070341059284432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)