FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236015
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89028217.00304525483598No Hit
CAACGGAATCCCAAAAGCAGCTG69933313.356206962737884No Hit
CCCCCACTGCTAAATTTGACTG2943715.622042717601077No Hit
TTCAAGTAATCCAGGATAGGCT1370482.617410377930544No Hit
AACATTCAACGCTGTCGGTGAG1240912.369951193799101No Hit
TCGTACGACTCTTAGCGG884391.6890516929382364No Hit
TAGCAGCACGTAAATATTGGCG776381.482768861433743No Hit
TGAGGTAGTAGATTGTATAGTT773951.4781279274410024No Hit
TATTGCACTTGTCCCGGCCTGTAA578281.104427699309494No Hit
AAGCTGCCAGTTGAAGAACTGT546611.043942769453487No Hit
TAGCTTATCAGACTGATGTTGAC506970.9682363400410426No Hit
TATTGCACTTGTCCCGGCCTGTAT490310.936418249374763No Hit
TGAGGTAGTAGGTTGTATAGTT436820.8342604060530766No Hit
TATTGCACTTGTCCCGGCCTGT408750.780650933964093No Hit
TAGCTTATCAGACTGATGTTGA383280.7320070702624037No Hit
TAGCTTATCAGACTGATGTTGACT375910.7179314803338035No Hit
CACCCGGCTGTGTGCACATGTGC322090.6151433867168066No Hit
AACATTCAACGCTGTCGGTGAGT310990.5939440586018183No Hit
TATTGCACTTGTCCCGGCCTGTA291130.5560144499204069No Hit
CCCCCACTGCTAAATTTGACTGGCTT286150.5465034000093583No Hit
TTCTCACTACTGCACTTGACTAG280040.5348342202992161No Hit
TGAGAACTGAATTCCATGGGTT269800.5152773626507946No Hit
GCCTGTCTGAGCGTCGCT268300.5124125885812015No Hit
CTCCCACTGCTTCACTTGACTAG264830.505785411233543No Hit
CAACGGAATCCCAAAAGCAGCTGT253820.48475796956273043No Hit
ACCCCCCACTGCTAAATTTGACTG247550.47278321395183165No Hit
TGTAAACATCCTTGACTGGAAGCT246180.47016672030160345No Hit
TGAGGTAGTAGTTTGTACAGTT235310.4494066575439528No Hit
TTCAAGTAATTCAGGATAGGTT205510.39249314602803853No Hit
CTTCTCACTACTGCACTTGACTAG201770.3853503093478533No Hit
CAACGGAATCCCAAAAGCAGCT200430.3827911111790169No Hit
AGCAGCATTGTACAGGGCTATGA195250.37289809139202235No Hit
AACGGAATCCCAAAAGCAGCTG169790.3242733261841305No Hit
CTCCCACTGCTTCACTTGACTAGCCT167640.32016715001771384No Hit
CATTGCACTTGTCTCGGTCTGA164880.3148959657296628No Hit
CATAAAGTAGAAAGCACTACT164140.3134826771886635No Hit
TCGTTTCCCGGCCAATGCACCA162950.3112099564267864No Hit
CCCATAAAGTAGAAAGCACT157430.30066758785068415No Hit
CCCCCCACTGCTAAATTTGACTGG151520.28938037801648775No Hit
TGTAAACATCCCCGACTGGAAGCT150240.28693577081043503No Hit
ACTGGACTTGGAGTCAGAAGGC149270.2850832169120982No Hit
ACCGGGTGCTGTAGGCTT129820.24793664647637564No Hit
CCCATAAAGTAGAAAGCACTAC124210.23722239145609783No Hit
TGAGAACTGAATTCCATAGGCTGT121360.23177932072387114No Hit
GGAATACCGGGTGCTGTAGGCTTT111020.2120314781374767No Hit
GACCCGCGGGCGCTCTCCAGTCCT109680.2094722799686403No Hit
CCCCCCACTGCTAAATTTGACTGGCT107510.20532790681462906No Hit
CAAAGAATTCTCCTTTTGGGCT104150.19891081289874074No Hit
TAGCAGCACGTAAATATTGGC93960.1794494477193056No Hit
TGAGGTAGTAGATTGTATAGT93330.17824624261007657No Hit
CACTAGATTGTGAGCTCCTGGA91840.17540056703428086No Hit
CACTAGATTGTGAGCTCCTGGAA91270.1743119528878355No Hit
TAGCTTATCAGACTGATGTTGACA89330.1706068450911619No Hit
GGAATACCGGGTGCTGTAGGCTT89050.17007208726483786No Hit
ACCGGGTGCTGTAGGCTTT85220.16275736414047706No Hit
TGTAAACATCCCCGACTGGA81140.15496517867118412No Hit
CCCATAAAGTAGAAAGCACTA80710.15414394343790078No Hit
TTCACAGTGGCTAAGTTCCGC73270.13993466405271948No Hit
CAACACCAGTCGATGGGCTGT71130.13584758638010014No Hit
ACTGGACTTGGAGTCAGAAGGCT68460.1307482885362246No Hit
ATGCACCTGGGCAAGGATTCTGA67290.12851376476194207No Hit
TATTGCACTTGTCCCGGCCTGTAG66900.1277689235038479No Hit
TCCCACTGCTTCACTTGACTAG66210.1264511274318351No Hit
ACCGGGTGCTGTAGGCTTA65500.12509513437222775No Hit
TCGTTTCCCGGCCAACGCACCA65450.12499964190324131No Hit
AGCTCGGTCTGAGGCCCCTCAGT65280.12467496750868744No Hit
TATTGCACTTGTCCCGGCCTGTT64820.12379643679401224No Hit
CAAAGAATTCTCCTTTTGGGC61430.11732204739673205No Hit
TTCAAGTAATCCAGGATAGGC61070.11663450162002975No Hit
AATGCACCCGGGCAAGGATTCT59890.1143808793519499No Hit
TGTAAACATCCTACACTCTCAGCT59850.11430448537676076No Hit
TGTCAGTTTGTCAAATACCCCA58640.11199356762728908No Hit
TACCACCCTGAACGCGCCCGA54660.10439236709596897No Hit
CCCCCCACTGCTAAATTTGACTGT54340.1037812152944558No Hit
TTCACAGTGGCTAAGTTCTGC53320.10183316892713257No Hit
TGATGTAGTAGATTGTATAGTT52780.10080185026207908No Hit
CCCCCCACTGCTAAATTTGACTGTA52450.10017159996676861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)