FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872399
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG99344111.196982912964126No Hit
CAACGGAATCCCAAAAGCAGCTG4121954.645812254385764No Hit
AACATTCAACGCTGTCGGTGAGT3121493.5182029121999587No Hit
CCCCCACTGCTAAATTTGACTG2957843.333754489625636No Hit
AAGCTGCCAGTTGAAGAACTGT2869393.2340633012559508No Hit
TAGCAGCACGTAAATATTGGCG2617922.950633757566584No Hit
TTCAAGTAATCCAGGATAGGCT2442772.7532237898678815No Hit
TAGCTTATCAGACTGATGTTGAC2108312.3762569740157087No Hit
TAGCTTATCAGACTGATGTTGACT1729871.9497207012443871No Hit
TAGCTTATCAGACTGATGTTGA1532711.7275034632685027No Hit
AACATTCAACGCTGTCGGTGAG1505431.6967564240517135No Hit
TGAGGTAGTAGGTTGTATAGTT946881.0672198128150008No Hit
TATTGCACTTGTCCCGGCCTGTAA903421.01823644315365No Hit
TATTGCACTTGTCCCGGCCTGTAT873100.9840630476605031No Hit
TGTAAACATCCTTGACTGGAAGCT819560.9237186019249135No Hit
TATTGCACTTGTCCCGGCCTGT701930.7911389016657163No Hit
AGCAGCATTGTACAGGGCTATGA668730.7537194844370727No Hit
CATAAAGTAGAAAGCACTACT636320.71719046900393No Hit
CGCCTGTCTGAGCGTCGCT603210.6798724899545208No Hit
TGAGGTAGTAGATTGTATAGTT590550.6656035194088994No Hit
TATTGCACTTGTCCCGGCCTGTA505580.5698346073029403No Hit
TTCAAGTAATTCAGGATAGGTT500690.564323132897878No Hit
TGTAAACATCCCCGACTGGAAGCT429490.4840742622147629No Hit
ACTGGACTTGGAGTCAGAAGGC375120.42279433104845715No Hit
TTCTCACTACTGCACTTGACTAG325380.36673283065831463No Hit
GCCTGTCTGAGCGTCGCT281670.31746768827686855No Hit
TCGTACGACTCTTAGCGG277870.31318474293142134No Hit
CCCATAAAGTAGAAAGCACTAC276580.3117307956957301No Hit
CTTCTCACTACTGCACTTGACTAG274050.3088792557683666No Hit
CAACGGAATCCCAAAAGCAGCTGT263030.29645871426656983No Hit
AACATTCAACGCTGTCGGTGAGTT250680.2825391418938666No Hit
TAGCTTATCAGACTGATGTTGACA246950.2783350929100461No Hit
CATTGCACTTGTCTCGGTCTGA241170.2718205076214449No Hit
TGTAAACATCCTACACTCTCAGCT238180.2684505058891062No Hit
TGAGGTAGTAGTTTGTACAGTT231230.2606172242704594No Hit
CCCCCCACTGCTAAATTTGACTGG227160.25602996438730946No Hit
CCCCCCACTGCTAAATTTGACTGTA215680.24309096108053752No Hit
CCCATAAAGTAGAAAGCACT208580.23508861582983362No Hit
CAACACCAGTCGATGGGCTGT205650.2317862395503178No Hit
TACGCCTGTCTGAGCGTCGCT201740.22737931420802873No Hit
TGATGTAGTAGGTTGTATAGTT186620.21033770009667058No Hit
TTCAAGTAATCCAGGATAGGCTT175160.19742123860750624No Hit
GCGTTCGCGCTTTCCCCTG172690.1946373241329656No Hit
CTACGCCTGTCTGAGCGTCGCT170270.19190976420244402No Hit
CCCCCACTGCTAAATTTGACTGGCTT169860.19144765694148785No Hit
TTATAAAGCAATGAGACTGATT163800.18461748620638002No Hit
CTCCCACTGCTTCACTTGACTAG157940.17801273364734838No Hit
CAACGGAATCCCAAAAGCAGCT157810.17786621183289886No Hit
ACCCCCCACTGCTAAATTTGACTG156470.17635591005318854No Hit
ACGCCTGTCTGAGCGTCGCT154470.1741017282924269No Hit
TGAGGTAGTAGTTTGTGCTGTT151450.17069791383367677No Hit
CAAAGAATTCTCCTTTTGGGCT150260.16935667568602358No Hit
ACCGGGTGCTGTAGGCTT149190.1681506884440161No Hit
TGTCAGTTTGTCAAATACCCCAA147080.16577252668641254No Hit
CCCCCCACTGCTAAATTTGACTGT140110.15791670325015816No Hit
AGCAGCATTGTACAGGGCTATGT139080.15675579964336592No Hit
TAGCAGCACGTAAATATTGGC137900.15542583240451652No Hit
CCCCCCACTGCTAAATTTGACTGGCT135420.15263064702117207No Hit
CAAAGAATTCTCCTTTTGGGCTT132370.14919301983601052No Hit
CGCGACCTCAGATCAGACGTGGCG130620.1472206107953441No Hit
TTCACAGTGGCTAAGTTCCG129880.14638656354386226No Hit
TATTGCACTTGTCCCGGCCTGTT128660.14501151266979764No Hit
TGTCAGTTTGTCAAATACCCCA121580.13703170923670138No Hit
CTCCCCACTGCTAAATTTGACTG119970.13521709291928824No Hit
TGATGTAGTAGATTGTATAGTT117790.13276003480005802No Hit
CCCGCCACTGCTAAATTTGACTG117260.13216267663345618No Hit
AGCTCGGTCTGAGGCCCCTCAGT114070.12856725672504135No Hit
ACCGGGTGCTGTAGGCTTT113020.12738381130064147No Hit
TGTAAACATCCTACACTCAGCT111070.12518598408389883No Hit
TAGCAGCACGTAAATATTGGCGT111000.1251070877222722No Hit
ACCACAGGGTAGAACCACGGAC110660.1247238768229427No Hit
TGTAAACATCCCCGACTGGAAGC109210.1230895950463905No Hit
AGCAGCATTGTACAGGGCTATG108420.12219919325088964No Hit
CACTAGATTGTGAGCTCCTGGA103500.11665390611941596No Hit
CACCCGGCTGTGTGCACATGTGC103430.1165750097577893No Hit
ACCGGGTGCTGTAGGCTTA103090.11619179885845983No Hit
TTCAAGTAATCCAGGATAGGC102930.11601146431759889No Hit
TCCTGTCTGAGCGTCGCT102770.11583112977673796No Hit
TATTGCACTTGTCCCGGCCTGTAG101930.11488437343721805No Hit
CGCCCCACTGCTAAATTTGACTG101180.11403905527693242No Hit
CCACCCACTGCTAAATTTGACTG100300.11304721530219729No Hit
CGCGACCTCAGATCAGACGTGG99740.11241604440918403No Hit
CCCCCACTGCTAAATTTGACTGTA99690.112359689865165No Hit
TGAGGTAGTAGGTTGTGTGGTT99400.11203283350985456No Hit
AACATTCAACGCTGTCGGTGA95720.1078851390700531No Hit
CCCACCACTGCTAAATTTGACTG95280.10738921908268553No Hit
AACGGAATCCCAAAAGCAGCTG95110.10719761363302079No Hit
ACTGGACTTGGAGTCAGAAGGCA93910.10584510457656379No Hit
AACATTCAACGCTGTCGGTGAGA91500.103128815554846No Hit
CACTAGATTGTGAGCTCCTGGAA91100.10267797920269367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)