FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8495099
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG100001911.771716845206866No Hit
CAACGGAATCCCAAAAGCAGCTG5197206.11788043906257No Hit
CCCCCACTGCTAAATTTGACTG3323683.912467647522413No Hit
TAGCAGCACGTAAATATTGGCG2361252.7795438287417253No Hit
TTCAAGTAATCCAGGATAGGCT2261952.6626529013964406No Hit
AAGCTGCCAGTTGAAGAACTGT2045072.4073527571603344No Hit
AACATTCAACGCTGTCGGTGAGT1895222.230956931755592No Hit
AACATTCAACGCTGTCGGTGAG1664351.9591884685511023No Hit
TATTGCACTTGTCCCGGCCTGTAT1450901.7079259464780812No Hit
TAGCTTATCAGACTGATGTTGA1223081.439747788695576No Hit
TAGCTTATCAGACTGATGTTGACT1213501.428470698222587No Hit
TATTGCACTTGTCCCGGCCTGTAA1124731.323975153203041No Hit
TAGCTTATCAGACTGATGTTGAC1114081.3114385129590602No Hit
TATTGCACTTGTCCCGGCCTGT1089761.2828102415286744No Hit
GCCTGTCTGAGCGTCGCT1087771.280467714384494No Hit
TGAGGTAGTAGGTTGTATAGTT1013931.1935470086928945No Hit
TATTGCACTTGTCCCGGCCTGTA792440.932820206097657No Hit
CATAAAGTAGAAAGCACTACT666290.7843228195457168No Hit
TGAGGTAGTAGATTGTATAGTT650700.765971061667439No Hit
TTCAAGTAATTCAGGATAGGTT610580.7187438310018518No Hit
TGTAAACATCCTTGACTGGAAGCT570400.6714459713771435No Hit
GGAATACCGGGTGCTGTAGGCTTT517360.6090099715141637No Hit
ATCCCGGACGAGCCCCCA481340.5666090530551792No Hit
AGCAGCATTGTACAGGGCTATGA462460.5443844739184323No Hit
GGAATACCGGGTGCTGTAGGCTT415520.4891290848994226No Hit
TCGTACGACTCTTAGCGG412860.48599786771172415No Hit
TGTAAACATCCCCGACTGGAAGCT379210.44638679313801993No Hit
ACCGGGTGCTGTAGGCTTA362840.42711685879116884No Hit
TTCTCACTACTGCACTTGACTAG355100.4180057230645576No Hit
TCCTGTCTGAGCGTCGCT342310.4029499832785939No Hit
ACCGGGTGCTGTAGGCTT330810.38941276611373216No Hit
CATTGCACTTGTCTCGGTCTGA324280.38172598106272804No Hit
TTAATGCTAATCGTGATAGGGGTT316920.37306216207721654No Hit
CAACACCAGTCGATGGGCTGT281830.3317559924846079No Hit
CAACGGAATCCCAAAAGCAGCTGT276610.32561127304107934No Hit
TGAGAACTGAATTCCATGGGTT270000.31783031604458056No Hit
TGATGTAGTAGGTTGTATAGTT268460.31601750609380774No Hit
ACCGGGTGCTGTAGGCTTT264220.31102639298258916No Hit
CCCATAAAGTAGAAAGCACT259290.30522304684147883No Hit
CCCATAAAGTAGAAAGCACTAC242650.2856352821785832No Hit
ACTGGACTTGGAGTCAGAAGGC224180.2638933342624965No Hit
TGAGGTAGTAGTTTGTACAGTT220820.2599381125517195No Hit
TATTGCACTTGTCCCGGCCTGTT219580.25847844739655185No Hit
CCCCCCACTGCTAAATTTGACTGT215960.25421716686291707No Hit
CCCCCCACTGCTAAATTTGACTGG208820.24581232072751594No Hit
CTTCTCACTACTGCACTTGACTAG206350.2429047619103674No Hit
CTCCCACTGCTTCACTTGACTAG205880.24235150173058606No Hit
CGCCTGTCTGAGCGTCGCT203280.23929091350200862No Hit
TGAATACCGGGTGCTGTAGGCTTT193920.22827279587912985No Hit
AGCTCGGTCTGAGGCCCCTCAGT188510.2219044180650514No Hit
CCCCCCACTGCTAAATTTGACTGTA185820.2187378863977924No Hit
ACCCCCCACTGCTAAATTTGACTG183400.21588918504657803No Hit
CACTAGATTGTGAGCTCCTGGA176350.2075902823498584No Hit
TGATGTAGTAGATTGTATAGTT173440.20416477783248907No Hit
CAACGGAATCCCAAAAGCAGCT172910.20354088869358675No Hit
CACCCGGCTGTGTGCACATGTGC164970.1941943231032387No Hit
TATTGCACTTGTCCCGGCCTGTAG164390.1935115764984022No Hit
TAGCAGCACGTAAATATTGGC163450.1924050561388396No Hit
CCCCCACTGCTAAATTTGACTGGCTT160830.18932092492388847No Hit
TAGCTTATCAGACTGATGTTGACA154890.1823286579709077No Hit
TGAATACCGGGTGCTGTAGGCTT154450.1818107122706869No Hit
CACTAGATTGTGAGCTCCTGGAA153790.1810337937203557No Hit
CAAAGAATTCTCCTTTTGGGCT149480.17596028015682924No Hit
TGTAAACATCCTACACTCTCAGCT147680.17384141138319872No Hit
TGAGGTAGTAGTTTGTGCTGTT140970.16594273945483154No Hit
TTCACAGTGGCTAAGTTCCG139710.16445953131329016No Hit
AACCGGGCGGAAACACCA138550.16309403810361717No Hit
CCCCCCACTGCTAAATTTGACTGA135850.15991573494317135No Hit
TATTGCACTTGTCCCGGCCTGTTT134080.15783218064910132No Hit
AACGGAATCCCAAAAGCAGCTG125110.14727315126050916No Hit
ATCCCACCGCTGCCACCA119750.14096363091236488No Hit
TTCAAGTAATCCAGGATAGGC119240.14036328475983623No Hit
TGTAAACATCCCCGACTGGAAGC118100.1390213345365369No Hit
TTCAAGTAATCCAGGATAGGCTT114210.13444222368685757No Hit
TATTGCACTTGTCCCGGCCTGA112860.13285307210663466No Hit
TTATAAAGCAATGAGACTGATT110970.13062825989432258No Hit
AACATTCAACGCTGTCGGTGAGTT110060.1295570540143205No Hit
TTCACAGTGGCTAAGTTCTG105830.12457771239628873No Hit
TCGTTTCCCGGGCGGCGCACCA105710.1244364544780467No Hit
AGCAGCATTGTACAGGGCTATGT104780.12334170561167093No Hit
TGAGGTAGTAGGTTGTATAGT100650.1184800789255075No Hit
CCCCCCACTGCTAAATTTGACTGGCT98240.11564314906747997No Hit
CAAAGAATTCTCCTTTTGGGCTT97260.11448954273517No Hit
TGTCAGTTTGTCAAATACCCCAA95560.11248838889340784No Hit
TGTCAGTTTGTCAAATACCCCA94540.11128769658835054No Hit
TGAGAACTGAATTCCATAGGCTGT92530.10892162645779645No Hit
GGAATACCGGGTGCTGTAGGCTTA92010.10830950881208093No Hit
TGAGGTAGTAGGTTGTGTGGTT89490.10534309252899819No Hit
TTCACAGTGGCTAAGTTCCGC88190.10381279841470947No Hit
TTCACAGTGGCTAAGTTCTGC88120.1037303979624016No Hit
TATTGCACTTGTCCCGGCCTGTAAT87950.10353028257822539No Hit
CGCGACCTCAGATCAGACGTGG87280.10274159253470735No Hit
CGCGACCTCAGATCAGACGTGGCG86480.10179987307976045No Hit
AGCAGCATTGTACAGGGCTATG85710.10089346810437406No Hit
GACCCGCGGGCGCTCTCCAGTCCT85240.10034020792459275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)