FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7538176
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG130265317.2807453686409No Hit
CCCCCACTGCTAAATTTGACTG5359647.109995839842423No Hit
CAACGGAATCCCAAAAGCAGCTG4751356.303049968586564No Hit
AACATTCAACGCTGTCGGTGAGT2265833.0058067097398626No Hit
AACATTCAACGCTGTCGGTGAG1862632.4709293070366094No Hit
TTCAAGTAATCCAGGATAGGCT1593472.113866802791551No Hit
TAGCTTATCAGACTGATGTTGACT1481901.9658601762548393No Hit
TAGCTTATCAGACTGATGTTGAC1451991.9261821427358554No Hit
TAGCTTATCAGACTGATGTTGA1426111.8918502300821842No Hit
AAGCTGCCAGTTGAAGAACTGT1403931.8624266666100657No Hit
TAGCAGCACGTAAATATTGGCG1373171.8216210393601848No Hit
TATTGCACTTGTCCCGGCCTGTAA858311.1386176178428309No Hit
TATTGCACTTGTCCCGGCCTGTAT795881.0557991747605788No Hit
TGAGGTAGTAGGTTGTATAGTT767751.0184824551721796No Hit
TATTGCACTTGTCCCGGCCTGTA542020.7190333576716701No Hit
TATTGCACTTGTCCCGGCCTGT540320.7167781702098758No Hit
GGAATACCGGGTGCTGTAGGCTTT536320.7114718467703592No Hit
TGTAAACATCCTTGACTGGAAGCT455160.6038065441825714No Hit
CATAAAGTAGAAAGCACTACT441260.585367070230252No Hit
TGAGGTAGTAGATTGTATAGTT429840.5702175168104326No Hit
GGAATACCGGGTGCTGTAGGCTT376780.4998291363852476No Hit
AGCAGCATTGTACAGGGCTATGA375430.4980382522244108No Hit
CAACACCAGTCGATGGGCTGT358030.47495574526251444No Hit
TGAATACCGGGTGCTGTAGGCTTT348100.461782797323915No Hit
TGTAAACATCCCCGACTGGAAGCT344440.45692751137675747No Hit
TCGTACGACTCTTAGCGG339480.45034767031175715No Hit
TTCAAGTAATTCAGGATAGGTT315970.4191597542959995No Hit
CATTGCACTTGTCTCGGTCTGA288150.38225427477416285No Hit
TGATGTAGTAGGTTGTATAGTT279570.3708722109964002No Hit
CAACGGAATCCCAAAAGCAGCT250090.3317646072471643No Hit
TGAATACCGGGTGCTGTAGGCTT248650.3298543308089384No Hit
CAACGGAATCCCAAAAGCAGCTGT218740.2901762972899545No Hit
CCCATAAAGTAGAAAGCACT209090.2773747919921212No Hit
ACTGGACTTGGAGTCAGAAGGC208520.27661864090199006No Hit
TAGCTTATCAGACTGATGTTGACA208480.27656557766759493No Hit
CCCGCCACTGCTAAATTTGACTG204940.2718694814236229No Hit
CCCCCACTGCTAAATTTGACTGGCTT203320.2697204204306188No Hit
CTCCCCACTGCTAAATTTGACTG194550.25808630628947904No Hit
TTCTCACTACTGCACTTGACTAG193350.2564944092576241No Hit
ACCGGGTGCTGTAGGCTT192110.254849448991374No Hit
CGCCCCACTGCTAAATTTGACTG175990.23346496553012294No Hit
CCCATAAAGTAGAAAGCACTAC173880.23066587991577803No Hit
ACCCCCCACTGCTAAATTTGACTG167100.22167166168579772No Hit
CAAAGAATTCTCCTTTTGGGCT160230.2125580511784283No Hit
TGATGTAGTAGATTGTATAGTT156910.20815380272362968No Hit
CCCCCCACTGCTAAATTTGACTGG151740.20129537967805475No Hit
AACATTCAACGCTGTCGGTGAGTT149190.19791259848536305No Hit
CTTCTCACTACTGCACTTGACTAG149050.19772687716497994No Hit
TGAGGTAGTAGTTTGTACAGTT140830.18682238249677377No Hit
CACTAGATTGTGAGCTCCTGGA140710.18666319279358826No Hit
ACCGGGTGCTGTAGGCTTT138020.18309469028051348No Hit
GCCTGTCTGAGCGTCGCT137050.18180790684643075No Hit
TGTCAGTTTGTCAAATACCCCAA136210.18069357892413232No Hit
CACCCGGCTGTGTGCACATGTGC134380.17826593595055357No Hit
CACTAGATTGTGAGCTCCTGGAA127480.16911252801738774No Hit
CCCCCCACTGCTAAATTTGACTGTA126320.16757369421992802No Hit
TTCACAGTGGCTAAGTTCTG120620.16001218331861713No Hit
ACCGGGTGCTGTAGGCTTA118910.15774373004822387No Hit
TAGCAGCACGTAAATATTGGC115090.15267619116348569No Hit
TATTGCACTTGTCCCGGCCTGTT111680.14815255043129796No Hit
TATTGCACTTGTCCCGGCCTGTAG108130.14344318837872716No Hit
TTATAAAGCAATGAGACTGATT107950.14320440382394892No Hit
TGAGGTAGTAGTTTGTGCTGTT107650.1428064295659852No Hit
TCGTTTCCCGGCCAATGCACCA106610.14142678547171092No Hit
GCCCCCACTGCTAAATTTGACTG101840.13509899477008763No Hit
CGCCTGTCTGAGCGTCGCT99980.1326315543707125No Hit
TCCTGTCTGAGCGTCGCT98390.13052229080350472No Hit
AACGGAATCCCAAAAGCAGCTG95270.12638335852068192No Hit
TTCACAGTGGCTAAGTTCCG95030.12606497911431094No Hit
CCCCCCACTGCTAAATTTGACTGT92730.123013843136589No Hit
TGTAAACATCCTACACTCTCAGCT90710.12033414979963322No Hit
TGTCAGTTTGTCAAATACCCCA90620.1202147575222441No Hit
CCCATAAAGTAGAAAGCACTA89950.11932594834612512No Hit
CCCCCCACTGCTAAATTTGACTGA88720.11769425388847381No Hit
CTCCCACTGCTTCACTTGACTAG85300.1131573473476873No Hit
CAAAGAATTCTCCTTTTGGGCTT84660.11230833559736465No Hit
TTCAAGTAATCCAGGATAGGCTT84050.11149912127283843No Hit
CCCGCACTGCTAAATTTGACTG81910.10866023823269715No Hit
GGAATACCGGGTGCTGTAGGCTTA81780.10848778272091286No Hit
ACTTGACTTGGAGTCAGAAGGC81670.10834185882632615No Hit
CCCCCCACTGCTAAATTTGACTGGCT80930.10736018899001562No Hit
AGCAGCATTGTACAGGGCTATGT79460.10541011512599334No Hit
TGCAAGTAATCCAGGATAGGCT76280.1011915879915778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)