FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8312816
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89446110.760024039988375No Hit
CAACGGAATCCCAAAAGCAGCTG6030687.254677596617079No Hit
AACATTCAACGCTGTCGGTGAGT3062573.684154683563307No Hit
TAGCAGCACGTAAATATTGGCG2361482.8407702035026396No Hit
AACATTCAACGCTGTCGGTGAG2185312.6288444252825998No Hit
AAGCTGCCAGTTGAAGAACTGT2173032.6140720545240024No Hit
CCCCCACTGCTAAATTTGACTG2134662.5679144107123264No Hit
TTCAAGTAATCCAGGATAGGCT2053862.470715098229048No Hit
TATTGCACTTGTCCCGGCCTGTAT1971342.371446691470135No Hit
TATTGCACTTGTCCCGGCCTGTAA1940812.334720268077629No Hit
TAGCTTATCAGACTGATGTTGACT1736482.08891908590302No Hit
TAGCTTATCAGACTGATGTTGAC1493361.796455015965709No Hit
TAGCTTATCAGACTGATGTTGA963031.1584882908511387No Hit
TATTGCACTTGTCCCGGCCTGT840101.0106081982327046No Hit
TGAGGTAGTAGGTTGTATAGTT780540.9389597941299314No Hit
TGTAAACATCCTTGACTGGAAGCT776420.9340035915627147No Hit
TATTGCACTTGTCCCGGCCTGTA763560.9185335029669849No Hit
AGCAGCATTGTACAGGGCTATGA628400.7559411876793616No Hit
TGAGGTAGTAGATTGTATAGTT567200.6823199262440068No Hit
TGTAAACATCCCCGACTGGAAGCT518000.6231342062665648No Hit
ACTGGACTTGGAGTCAGAAGGC459270.5524842604479637No Hit
CATAAAGTAGAAAGCACTACT421270.5067717124979068No Hit
TTCAAGTAATTCAGGATAGGTT419550.5047026182222727No Hit
CAACGGAATCCCAAAAGCAGCTGT358850.43168283768099763No Hit
GGAATACCGGGTGCTGTAGGCTTT340730.40988517007954944No Hit
CCCCCACTGCTAAATTTGACTGGCTT336890.40526579681301733No Hit
CCCATAAAGTAGAAAGCACTAC329220.3960390798978349No Hit
TAGCTTATCAGACTGATGTTGACA326340.39257454994793584No Hit
CTCCCACTGCTTCACTTGACTAG315620.37967879957886713No Hit
ACCCCCCACTGCTAAATTTGACTG296390.35654584439256204No Hit
TTCTCACTACTGCACTTGACTAG275490.3314039430200308No Hit
TATTGCACTTGTCCCGGCCTGTAG261340.3143820337175754No Hit
CTTCTCACTACTGCACTTGACTAG253270.30467413208712907No Hit
GGAATACCGGGTGCTGTAGGCTT243530.2929572842704566No Hit
TGAGGTAGTAGTTTGTACAGTT239630.28826573329663496No Hit
CATTGCACTTGTCTCGGTCTGA238850.28732742310187065No Hit
CAAAGAATTCTCCTTTTGGGCT237260.2854147138586972No Hit
CACTAGATTGTGAGCTCCTGGAA234100.28161335460811354No Hit
CCCCCCACTGCTAAATTTGACTGGCT231170.27808867656880654No Hit
AACATTCAACGCTGTCGGTGAGTT227750.2739745472533014No Hit
CCCCCCACTGCTAAATTTGACTGG227340.27348133292015603No Hit
AGCTCGGTCTGAGGCCCCTCAGT219580.2641463494440392No Hit
CACTAGATTGTGAGCTCCTGGA213630.2569887268044908No Hit
CCCCCCACTGCTAAATTTGACTGT211100.25394523348044756No Hit
TATTGCACTTGTCCCGGCCTGTT197850.2380059897873356No Hit
CCCCCCACTGCTAAATTTGACTGTA197060.2370556499746897No Hit
CCCATAAAGTAGAAAGCACT196910.23687520570646578No Hit
CGCGACCTCAGATCAGACGTGGCG184270.22166976870413108No Hit
TGTAAACATCCTACACTCTCAGCT183500.2207434881272483No Hit
CGCCTGTCTGAGCGTCGCT181580.21843380149398228No Hit
TATTGCACTTGTCCCGGCCTGTAAT180960.21768796518532346No Hit
CTCCCACTGCTTCACTTGACTAGCCT173250.20841312979861457No Hit
TATTGCACTTGTCCCGGCCTGTTT173240.20840110018073296No Hit
CAACACCAGTCGATGGGCTGT171310.20607938392958539No Hit
ACTGGACTTGGAGTCAGAAGGCA171040.2057545842467823No Hit
CACCCGGCTGTGTGCACATGTGC165480.1990661167046161No Hit
TCGTACGACTCTTAGCGG150640.18121416376833074No Hit
TGAGGTAGTAGTTTGTGCTGTT149020.17926536567151252No Hit
TATTGCACTTGTCCCGGCCTGTAAA144030.17326258634859715No Hit
GCCTGTCTGAGCGTCGCT138870.1670553035216947No Hit
AGCAGCATTGTACAGGGCTATGT134480.16177430127167497No Hit
TAGCAGCACGTAAATATTGGC133900.1610765834345425No Hit
CAAAGAATTCTCCTTTTGGGCTT130540.15703463182632696No Hit
TTCAAGTAATCCAGGATAGGCTT125630.15112808944646436No Hit
TCGTTTCCCGGGCGGCGCACCA125350.1507912601457797No Hit
TGTCAGTTTGTCAAATACCCCAA125220.150634875113319No Hit
AACGGAATCCCAAAAGCAGCTG121820.14654480503357709No Hit
TGTAAACATCCCCGACTGGAAGC119520.14377799292081048No Hit
ATCCCGGACGAGCCCCCA118620.142695327311467No Hit
ACTGGACTTGGAGTCAGAAGGCT116350.1399646040523452No Hit
ACTGGACTTGGAGTCAGAAGG115260.13865337570325148No Hit
AACATTCAACGCTGTCGGTGAGA104670.1259140103666435No Hit
CAAAGTGCTGTTCGTGCAGGTAG104300.12546891450502454No Hit
TTCACAGTGGCTAAGTTCCG101660.12229309538428373No Hit
TGAGGTAGTAGGTTGTGTGGTT99090.11920148358871412No Hit
TTCAAGTAATCCAGGATAGGC98040.11793837371114674No Hit
CTACGCCTGTCTGAGCGTCGCT91910.11056421794972968No Hit
TGTCAGTTTGTCAAATACCCCA89630.10782146507272625No Hit
CAACGGAATCCCAAAAGCAGCT88020.10588469659378963No Hit
ATGCACCTGGGCAAGGATTCTGA87750.10555989691098659No Hit
TGAATACCGGGTGCTGTAGGCTTT85410.10274496632669364No Hit
TATTGCACTTGTCCCGGCCTGA84930.10216754466837713No Hit
ACCGGGTGCTGTAGGCTT84650.10183071536769249No Hit
AGCAGCATTGTACAGGGCTATG83930.10096458288021773No Hit
TGATGTAGTAGGTTGTATAGTT83710.10069993128682266No Hit
ACCGGGTGCTGTAGGCTTA83330.1002428058073221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)