FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18833635
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG15457838.20756587881203No Hit
CCCCCCACTGCTAAATTTGACTG11206695.95035955618764No Hit
AACATTCAACGCTGTCGGTGAGT8292554.40305336702129No Hit
AAGCTGCCAGTTGAAGAACTGT6197713.290766758514753No Hit
AACATTCAACGCTGTCGGTGAG6132903.2563549203326922No Hit
TTCAAGTAATCCAGGATAGGCT4683252.4866415856524777No Hit
TAGCAGCACGTAAATATTGGCG4500332.3895174776404025No Hit
CCCCCACTGCTAAATTTGACTG3586021.9040509174145088No Hit
TATTGCACTTGTCCCGGCCTGTAA3186421.6918773247968328No Hit
TAGCTTATCAGACTGATGTTGAC2895131.5372125455335628No Hit
TATTGCACTTGTCCCGGCCTGTAT2668891.4170870360395111No Hit
TAGCTTATCAGACTGATGTTGACT2663101.4140127489993302No Hit
TATTGCACTTGTCCCGGCCTGT2376851.262024033066373No Hit
TAGCTTATCAGACTGATGTTGA2153651.1435126570096532No Hit
TGAGGTAGTAGGTTGTATAGTT1588510.8434431271499102No Hit
TATTGCACTTGTCCCGGCCTGTA1478900.7852440593650667No Hit
CGCCTGTCTGAGCGTCGCT1431930.7603046358283996No Hit
TCGTACGACTCTTAGCGG1278290.6787271814495714No Hit
TGTAAACATCCTTGACTGGAAGCT1265120.6717343731042892No Hit
AGCAGCATTGTACAGGGCTATGA1166590.6194183969265625No Hit
CATAAAGTAGAAAGCACTACT1098640.5833393288125208No Hit
ACTGGACTTGGAGTCAGAAGGC1016840.5399063961895831No Hit
TGTAAACATCCCCGACTGGAAGCT981310.5210412116407692No Hit
CAACGGAATCCCAAAAGCAGCTGT881350.4679659555895609No Hit
TGAGGTAGTAGATTGTATAGTT864460.45899795764333334No Hit
TTAATGCTAATCGTGATAGGGGTT770190.40894389213765686No Hit
TTCAAGTAATTCAGGATAGGTT741490.3937051981733744No Hit
CCCATAAAGTAGAAAGCACT649980.34511659592001226No Hit
CATTGCACTTGTCTCGGTCTGA628040.3334672249940067No Hit
CAACACCAGTCGATGGGCTGT588490.31246756136030035No Hit
CAACGGAATCCCAAAAGCAGCT576230.3059579311163246No Hit
CCCATAAAGTAGAAAGCACTAC565770.3004040377760321No Hit
AACATTCAACGCTGTCGGTGAGTT557460.29599171907069455No Hit
GCCTGTCTGAGCGTCGCT548840.29141480123194485No Hit
GGAATACCGGGTGCTGTAGGCTTT530000.28141142164006044No Hit
TAGCTTATCAGACTGATGTTGACA529010.2808857663430347No Hit
CACCCGGCTGTGTGCACATGTGC503120.26713908387839097No Hit
TTCTCACTACTGCACTTGACTAG457270.24279434108179326No Hit
CTCCCACTGCTTCACTTGACTAG456630.24245452351603927No Hit
TGAGGTAGTAGTTTGTACAGTT429890.22825652084687847No Hit
CACTAGATTGTGAGCTCCTGGA426850.22664238740954679No Hit
CGCGACCTCAGATCAGACGTGGCG425320.22583001104141603No Hit
CTACGCCTGTCTGAGCGTCGCT424240.22525656889920612No Hit
TACGCCTGTCTGAGCGTCGCT416800.22130618969731547No Hit
GGAATACCGGGTGCTGTAGGCTT412730.21914516236509837No Hit
ACCGGGTGCTGTAGGCTT412480.21901242112847574No Hit
TATTGCACTTGTCCCGGCCTGTT406600.21589034724311054No Hit
TATTGCACTTGTCCCGGCCTGTAG387500.20574891676513854No Hit
TGAGGTAGTAGTTTGTGCTGTT376810.20007290148715318No Hit
AACGGAATCCCAAAAGCAGCTG353760.187834159470543No Hit
CTTCTCACTACTGCACTTGACTAG346270.18385723202132778No Hit
ACGCCTGTCTGAGCGTCGCT344310.18281654072620607No Hit
CCCCCACTGCTAAATTTGACTGGCTT342270.1817333722353651No Hit
ACCCCCCACTGCTAAATTTGACTG340630.18086258972312036No Hit
CACTAGATTGTGAGCTCCTGGAA337060.17896704486414863No Hit
CAAAGAATTCTCCTTTTGGGCT330520.17549453411409957No Hit
CCCCCCACTGCTAAATTTGACTGG321690.17080611363658688No Hit
TGAGGTAGTAGGTTGTGTGGTT316540.16807164416215986No Hit
TGTAAACATCCTACACTCTCAGCT315810.1676840397512217No Hit
CGCGACCTCAGATCAGACGTGG304050.16143989198049127No Hit
TGATGTAGTAGGTTGTATAGTT301100.15987354538834378No Hit
ACTGGACTTGGAGTCAGAAGGCT300720.15967177870867733No Hit
ACTGGACTTGGAGTCAGAAGGCA292460.1552860082506643No Hit
CCCCCCACTGCTAAATTTGACTGTA290710.1543568195943056No Hit
GCGTTCGCGCTTTCCCCTG289620.15377806780263076No Hit
TAGCAGCACGTAAATATTGGC277030.1470932191263131No Hit
TGTCAGTTTGTCAAATACCCCAA275580.1463233199539016No Hit
TGAGAACTGAATTCCATGGGTT273010.14495874004142056No Hit
AGCTCGGTCTGAGGCCCCTCAGT269830.14327027151158023No Hit
ACTGGACTTGGAGTCAGAAGG267590.1420809100314411No Hit
TCGTTTCCCGGCCAATGCACCA261750.13898007474393553No Hit
ACCACAGGGTAGAACCACGGAC258430.13721727112158646No Hit
TTCAAGTAATCCAGGATAGGCTT256960.13643675265024516No Hit
TTCACAGTGGCTAAGTTCCG253910.1348173095634486No Hit
TGTAAACATCCCCGACTGGAAGC249590.13252354099460883No Hit
AACATTCAACGCTGTCGGTGAGA242880.1289607662036564No Hit
CCAAGTCCTTCTGATCGAGGCCC238820.1268050485209042No Hit
TATTGCACTTGTCCCGGCCTGTAAT237540.12612541338939615No Hit
ATCCCGGACGAGCCCCCA236400.12552011335039678No Hit
TTCAAGTAATCCAGGATAGGC232130.12325289302888157No Hit
TGAATACCGGGTGCTGTAGGCTTT230380.12232370437252288No Hit
AGCAGCATTGTACAGGGCTATGT230060.12215379558964587No Hit
AACATTCAACGCTGTCGGTGA221960.11785297952307135No Hit
AGCAGCATTGTACAGGGCTATG221800.11776802513163284No Hit
TTCACAGTGGCTAAGTTCTG220940.11731139527765086No Hit
CAAAGAATTCTCCTTTTGGGCTT219710.11665830839346733No Hit
ACCGGGTGCTGTAGGCTTT218480.11600522150928379No Hit
CCCCCCACTGCTAAATTTGACTGT216590.11500169776041641No Hit
ACCGGGTGCTGTAGGCTTA213680.11345658976612853No Hit
CAAAGTGCTGTTCGTGCAGGTAG213440.11332915817897075No Hit
TACCACCCTGAACGCGCCCGA213370.11329199063271642No Hit
TCCTGTCTGAGCGTCGCT207400.11012212990216706No Hit
ACTTGACTTGGAGTCAGAAGGC205190.10894869737042266No Hit
TATTGCACTTGTCCCGGCCTGTAAA194320.10317710840206896No Hit
CGCGACCTCAGATCAGACGTGGC194250.10313994085581463No Hit
CCCCCCACTGCTAAATTTGACTGGCT190230.10100546177092208No Hit
TATTGCACTTGTCCCGGCCTGTTT188580.10012936960921245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)