FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10017244
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8131858.117851576741069No Hit
CCCCCCACTGCTAAATTTGACTG7750427.7370781823823No Hit
AACATTCAACGCTGTCGGTGAGT3859943.8532953774511234No Hit
AAGCTGCCAGTTGAAGAACTGT3674023.6676954260073926No Hit
TTCAAGTAATCCAGGATAGGCT3630943.624689585279145No Hit
TAGCAGCACGTAAATATTGGCG2894122.8891379704836977No Hit
AACATTCAACGCTGTCGGTGAG2664412.6598234005281296No Hit
CCCCCACTGCTAAATTTGACTG2283312.279379438096946No Hit
TATTGCACTTGTCCCGGCCTGTAA2022622.0191381980912118No Hit
TAGCTTATCAGACTGATGTTGACT1966981.9635939785433998No Hit
TATTGCACTTGTCCCGGCCTGTAT1876761.873529286099051No Hit
TAGCTTATCAGACTGATGTTGAC1795311.7922194966998908No Hit
TAGCTTATCAGACTGATGTTGA1410051.4076226954240108No Hit
TGAGGTAGTAGGTTGTATAGTT1028651.0268792494222962No Hit
TATTGCACTTGTCCCGGCCTGT1002271.000544660786939No Hit
TGTAAACATCCTTGACTGGAAGCT979900.9782131692110125No Hit
CATAAAGTAGAAAGCACTACT864670.8631815297700645No Hit
TATTGCACTTGTCCCGGCCTGTA839490.8380448754168311No Hit
TTAATGCTAATCGTGATAGGGGTT760690.7593805242240281No Hit
AGCAGCATTGTACAGGGCTATGA687670.6864862231567884No Hit
TGAGGTAGTAGATTGTATAGTT659030.6578955249567645No Hit
TTCAAGTAATTCAGGATAGGTT631760.6306724683954987No Hit
TGTAAACATCCCCGACTGGAAGCT601730.6006941629853481No Hit
CCCATAAAGTAGAAAGCACTAC594410.5933867638643923No Hit
CAACGGAATCCCAAAAGCAGCTGT541960.5410270529498932No Hit
CAACGGAATCCCAAAAGCAGCT444640.44387458266964447No Hit
ACTGGACTTGGAGTCAGAAGGC440050.4392924840405205No Hit
CCCATAAAGTAGAAAGCACT373710.3730666838104373No Hit
TAGCTTATCAGACTGATGTTGACA364710.364082176694508No Hit
TCGTACGACTCTTAGCGG349470.3488684113115344No Hit
GCCTGTCTGAGCGTCGCT330240.3296715144404988No Hit
TTCTCACTACTGCACTTGACTAG312040.3115028444949529No Hit
CCCCCACTGCTAAATTTGACTGGCTT308130.30759957529236587No Hit
TCGTTTCCCGGCCAATGCACCA305910.3053833968704366No Hit
CAAAGAATTCTCCTTTTGGGCT296390.2958797848989203No Hit
TGTAAACATCCTACACTCTCAGCT294650.29414278018984064No Hit
CATTGCACTTGTCTCGGTCTGA287700.2872047441392063No Hit
TGAGGTAGTAGTTTGTACAGTT284160.2836708380069409No Hit
AACATTCAACGCTGTCGGTGAGTT273240.2727696360396133No Hit
CAACACCAGTCGATGGGCTGT243740.24332041827073392No Hit
CTTCTCACTACTGCACTTGACTAG243480.24306086584294043No Hit
CTCCCACTGCTTCACTTGACTAG241600.24118410213427965No Hit
CACCCGGCTGTGTGCACATGTGC237940.23753040257380176No Hit
TATTGCACTTGTCCCGGCCTGTAG232340.23194004259055684No Hit
ACCCCCCACTGCTAAATTTGACTG229740.2293445183126217No Hit
CACTAGATTGTGAGCTCCTGGA215810.21543849785430005No Hit
TATTGCACTTGTCCCGGCCTGTT210570.21020751815569233No Hit
TGAGAACTGAATTCCATGGGTT208080.20772180452028524No Hit
CCCCCCACTGCTAAATTTGACTGTA205740.2053858326701436No Hit
CACTAGATTGTGAGCTCCTGGAA205220.20486672781455656No Hit
TGATGTAGTAGGTTGTATAGTT202890.20254073875009934No Hit
TTCAAGTAATCCAGGATAGGCTT194620.19428497498912875No Hit
CACCCGTAGAACCGACCTTGCG187820.18749668072375994No Hit
TAGCAGCACGTAAATATTGGC181170.18085812824365663No Hit
TGAGGTAGTAGTTTGTGCTGTT179940.17963024560447963No Hit
CCCCCCACTGCTAAATTTGACTGG176980.17667534104190732No Hit
CAAAGAATTCTCCTTTTGGGCTT176010.17570701083052384No Hit
CCCCCCACTGCTAAATTTGACTGT173190.17289186526753267No Hit
TTCACAGTGGCTAAGTTCCG171700.17140443020056215No Hit
AGCAGCATTGTACAGGGCTATGT171560.17126467120098102No Hit
TTCAAGTAATCCAGGATAGGC167270.16698205614238806No Hit
TTAATGCTAATCGTGATAGGGGTTT164580.16429668679329365No Hit
AACGGAATCCCAAAAGCAGCTG164540.1642567556505562No Hit
TGTAAACATCCCCGACTGGAAGC161640.1613617478020901No Hit
TATTGCACTTGTCCCGGCCTGTAAT160780.1605032282332346No Hit
TCGTTTCCCGGGCGGCGCACCA160720.1604433315191284No Hit
ACTGGACTTGGAGTCAGAAGGCA159430.15915555216584523No Hit
TGAGAACTGAATTCCATAGGCTGT158930.15865641288162693No Hit
TATTGCACTTGTCCCGGCCTGTTT152850.15258687918553246No Hit
AGCTCGGTCTGAGGCCCCTCAGT150820.1505603736916062No Hit
CGCCTGTCTGAGCGTCGCT150770.15051045976318436No Hit
CCCCCCACTGCTAAATTTGACTGGCT145040.1447903235660427No Hit
CCCATAAAGTAGAAAGCACTA144800.14455073670961793No Hit
ACTGGACTTGGAGTCAGAAGGCT139590.13934970536806332No Hit
TATTGCACTTGTCCCGGCCTGTAAA138950.1387108070842639No Hit
CGCGACCTCAGATCAGACGTGGCG133220.13299067088712224No Hit
TGATGTAGTAGATTGTATAGTT129830.1296065065401222No Hit
CTCCCACTGCTTCACTTGACTAGCCT127710.12749015597503666No Hit
ATGCACCTGGGCAAGGATTCTGA121980.121770019777895No Hit
TCCTGTCTGAGCGTCGCT117480.11727776621993034No Hit
ATCCCGGACGAGCCCCCA117010.11680857529276516No Hit
AACATTCAACGCTGTCGGTGAGA116240.11603990079506898No Hit
TGAGGTAGTAGGTTGTGTGGTT116080.11588017622411913No Hit
CAACGGAATCCCAAAAGCAGCTGA115420.11522131236895099No Hit
TTATAAAGCAATGAGACTGATT113390.1131948068750247No Hit
TATTGCACTTGTCCCGGCCTGA111570.11137793988047011No Hit
TTCACAGTGGCTAAGTTCTGC111540.11134799152341703No Hit
TTCACAGTGGCTAAGTTCCGC110510.11031976459792733No Hit
ACCGGGTGCTGTAGGCTT109200.1090120196732754No Hit
ACTGGACTTGGAGTCAGAAGG107300.1071152903932459No Hit
GGAATACCGGGTGCTGTAGGCTTT107210.10702544532208659No Hit
TGTCAGTTTGTCAAATACCCCA107060.1068757035368211No Hit
AACCGGGCGGAAACACCA106220.10603714953933438No Hit
ACCACAGGGTAGAACCACGGAC105930.10574764875448776No Hit
TAGCAGCACGTAAATATTGGCGT105390.105208578327532No Hit
AGCAGCATTGTACAGGGCTATG104970.10478930132878865No Hit
CAAAGTGCTGTTCGTGCAGGTAG104270.10409050633088304No Hit
TTCACAGTGGCTAAGTTCTG103820.10364128097508656No Hit
CTACGCCTGTCTGAGCGTCGCT103600.10342165969003052No Hit
AACATTCAACGCTGTCGGTGA103150.10297243433423406No Hit
ATCACATTGCCAGGGATTTCC100980.10080616984072664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)