FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19603674
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG14396197.343618344194053No Hit
CAACGGAATCCCAAAAGCAGCTG12760806.5093920659974245No Hit
AACATTCAACGCTGTCGGTGAGT7601413.8775435665783875No Hit
AACATTCAACGCTGTCGGTGAG6182333.153658849866612No Hit
AAGCTGCCAGTTGAAGAACTGT5801662.9594758615145307No Hit
TTCAAGTAATCCAGGATAGGCT5606032.8596833430304955No Hit
TAGCAGCACGTAAATATTGGCG5256172.681216796402552No Hit
CCCCCACTGCTAAATTTGACTG4244002.1649003140941847No Hit
TATTGCACTTGTCCCGGCCTGTAT3336931.7021962311758498No Hit
TATTGCACTTGTCCCGGCCTGTAA3303901.6853473486653572No Hit
TATTGCACTTGTCCCGGCCTGT2708001.3813737159677315No Hit
TAGCTTATCAGACTGATGTTGACT2599761.3261595760060079No Hit
TAGCTTATCAGACTGATGTTGA2407511.2280912241246207No Hit
TATTGCACTTGTCCCGGCCTGTA2372421.2101915181817449No Hit
TAGCTTATCAGACTGATGTTGAC2253571.149565127434786No Hit
ATCCCGGACGAGCCCCCA2068761.055291982513074No Hit
TGAGGTAGTAGGTTGTATAGTT1959450.9995320264966658No Hit
TTAATGCTAATCGTGATAGGGGTT1847200.9422723516010315No Hit
CATAAAGTAGAAAGCACTACT1560480.7960140532840936No Hit
TGTAAACATCCTTGACTGGAAGCT1360570.6940382705813206No Hit
TGAGGTAGTAGATTGTATAGTT1224280.6245155882514676No Hit
TCGTACGACTCTTAGCGG1224180.6244645774052353No Hit
GCCTGTCTGAGCGTCGCT1121950.5723161893020665No Hit
AGCAGCATTGTACAGGGCTATGA1103220.5627618578027771No Hit
ACTGGACTTGGAGTCAGAAGGC1031560.5262074853927892No Hit
TGTAAACATCCCCGACTGGAAGCT994680.5073946853023571No Hit
ACCGGGTGCTGTAGGCTT938640.47880820707383726No Hit
TTCAAGTAATTCAGGATAGGTT876400.44705905637892157No Hit
CGCCTGTCTGAGCGTCGCT861640.43952985547505025No Hit
ACCGGGTGCTGTAGGCTTA812300.41436110394408715No Hit
CAACACCAGTCGATGGGCTGT796240.40616876203919733No Hit
CCCATAAAGTAGAAAGCACT721160.3678698186880684No Hit
CAACGGAATCCCAAAAGCAGCTGT686710.35029658216107856No Hit
CATTGCACTTGTCTCGGTCTGA654530.3338812918435595No Hit
TTCACAGTGGCTAAGTTCCG639280.3261021377931504No Hit
AACATTCAACGCTGTCGGTGAGTT626320.3194911321214584No Hit
CCCATAAAGTAGAAAGCACTAC626170.3194146158521102No Hit
ACCGGGTGCTGTAGGCTTT614590.31350755985842244No Hit
TGAGAACTGAATTCCATGGGTT595450.30374408388958113No Hit
TATTGCACTTGTCCCGGCCTGTT592050.3020097151176866No Hit
TTCTCACTACTGCACTTGACTAG576880.2942713697442632No Hit
CACTAGATTGTGAGCTCCTGGA571060.2913025384935497No Hit
TGAGGTAGTAGTTTGTACAGTT538080.2744791614061731No Hit
AGCTCGGTCTGAGGCCCCTCAGT535660.27324469892735415No Hit
CTCCCACTGCTTCACTTGACTAG529190.26994429717613133No Hit
TATTGCACTTGTCCCGGCCTGTAG505060.25763537998030367No Hit
CACTAGATTGTGAGCTCCTGGAA502210.2561815708626862No Hit
CAAAGAATTCTCCTTTTGGGCT461340.23533343800758982No Hit
TCGTTTCCCGGGCGGCGCACCA448210.2286357138973031No Hit
TGAGGTAGTAGTTTGTGCTGTT416120.21226633334139305No Hit
CTTCTCACTACTGCACTTGACTAG401770.20494627690707365No Hit
ACTGGACTTGGAGTCAGAAGGCA398040.20304357234261292No Hit
TAGCTTATCAGACTGATGTTGACA397290.20266099099587148No Hit
ACTGGACTTGGAGTCAGAAGG391740.1998298890299849No Hit
CCCCCACTGCTAAATTTGACTGGCTT373530.19054081393110292No Hit
AACGGAATCCCAAAAGCAGCTG368610.18803108029647914No Hit
ACTGGACTTGGAGTCAGAAGGCT367450.1874393544801857No Hit
TACGCCTGTCTGAGCGTCGCT365720.18655686684036882No Hit
TTCACAGTGGCTAAGTTCTG363050.1851948772459693No Hit
ACCCCCCACTGCTAAATTTGACTG360710.18400122344413603No Hit
TGTAAACATCCTACACTCTCAGCT354490.18082834880849377No Hit
CGCGACCTCAGATCAGACGTGGCG347050.17703314184881874No Hit
TATTGCACTTGTCCCGGCCTG346020.1765077301326272No Hit
CCCCCCACTGCTAAATTTGACTGT332580.16965187239902071No Hit
CCCCCCACTGCTAAATTTGACTGTA318540.1624899495880211No Hit
TTCAAGTAATCCAGGATAGGC316180.1612860936169414No Hit
CCCCCCACTGCTAAATTTGACTGG312510.15941399556021998No Hit
CACCCGGCTGTGTGCACATGTGC310800.1585417100896495No Hit
TGAGGTAGTAGGTTGTGTGGTT303910.15502706278425157No Hit
TAGCAGCACGTAAATATTGGC303070.15459857167590116No Hit
CTACGCCTGTCTGAGCGTCGCT290620.1482477213199934No Hit
TATTGCACTTGTCCCGGCCTGA284370.14505954343048147No Hit
TTAATGCTAATCGTGATAGGGGT280070.14286607704249724No Hit
TATTGCACTTGTCCCGGCCTGTTT278850.14224374471846452No Hit
AGCTACATTGTCTGCTGGGTTT269520.13748443276500108No Hit
AACCGGGCGGAAACACCA262570.13393917895186383No Hit
TTAATGCTAATCGTGATAGGGGTTT260850.13306179239667015No Hit
CCCATAAAGTAGAAAGCACTA258040.13162838761754556No Hit
TGTCAGTTTGTCAAATACCCCAA257840.13152636592508118No Hit
ATCCCACCGCTGCCACCA257400.13130191820165954No Hit
AGCAGCATTGTACAGGGCTATG257230.1312151997630648No Hit
GGAATACCGGGTGCTGTAGGCTTT253890.12951143749890964No Hit
AGCAGCATTGTACAGGGCTATGT247180.12608860971672964No Hit
GGAATACCGGGTGCTGTAGGCTT246800.1258947685010473No Hit
TCGTTTCCCGGCCAATGCACCA240920.12289533074259448No Hit
AACATTCAACGCTGTCGGTGAGA236100.12043660795420287No Hit
TCCTGTCTGAGCGTCGCT228080.11634553808638115No Hit
TTCACCACCTTCTCCACCCAGC226800.11569259925460912No Hit
TGAGGTAGTAGGTTGTATAGT226480.1155293645466661No Hit
CCCCCCACTGCTAAATTTGACTGGCT225730.11514678319992466No Hit
ACCACAGGGTAGAACCACGGAC224570.11455505738363127No Hit
CAACGGAATCCCAAAAGCAGCT223410.11396333156733784No Hit
TGAGAACTGAATTCCATAGGCTGT220080.11226467038780588No Hit
CAAAGTGCTGTTCGTGCAGGT220030.11223916496468979No Hit
TATTGCACTTGTCCCGGCCTGTAAT219310.11187188687181801No Hit
TATTGCACTTGTCCCGGCCTGTAAA217890.11114753285532089No Hit
TGTAAACATCCCCGACTGGAAGC216330.11035176365409871No Hit
TTCAAGTAATCCAGGATAGGCTT212750.10852557535898628No Hit
CGCGACCTCAGATCAGACGTGG212530.10841335149727546No Hit
ACGCCTGTCTGAGCGTCGCT205200.10467425646845586No Hit
AACATTCAACGCTGTCGGTGA203250.10367954496692815No Hit
TTATAAAGCAATGAGACTGATT198630.10132284387100092No Hit
AGCTACATCTGGCTACTGGGTCTC197920.10096066686275235No Hit
GACCCGCGGGCGCTCTCCAGTCCT196330.10014959440766054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)