FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5560902
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5260959.46060549169901No Hit
CAACGGAATCCCAAAAGCAGCTG4164427.488749127389766No Hit
AACATTCAACGCTGTCGGTGAGT2154473.8743175117993447No Hit
CCCCCACTGCTAAATTTGACTG1934403.4785723611025694No Hit
AAGCTGCCAGTTGAAGAACTGT1755153.1562325680258345No Hit
AACATTCAACGCTGTCGGTGAG1677473.0165429996788293No Hit
TTCAAGTAATCCAGGATAGGCT1494212.686992146238146No Hit
TAGCAGCACGTAAATATTGGCG1452612.6121841384724993No Hit
TAGCTTATCAGACTGATGTTGA779491.4017330282029787No Hit
TAGCTTATCAGACTGATGTTGAC715111.2859604431079708No Hit
TAGCTTATCAGACTGATGTTGACT650871.1704396157314048No Hit
CGCCTGTCTGAGCGTCGCT642751.1558376680617641No Hit
TATTGCACTTGTCCCGGCCTGT623301.1208613278924895No Hit
TGAGGTAGTAGGTTGTATAGTT589001.0591806868741798No Hit
TATTGCACTTGTCCCGGCCTGTAA588241.0578140021169227No Hit
TCGTACGACTCTTAGCGG529080.9514283833809695No Hit
TATTGCACTTGTCCCGGCCTGTAT503790.9059501498138253No Hit
CATAAAGTAGAAAGCACTACT419670.7546797264184839No Hit
TATTGCACTTGTCCCGGCCTGTA383800.690175802414788No Hit
CAACACCAGTCGATGGGCTGT324110.5828371008875898No Hit
AGCAGCATTGTACAGGGCTATGA323820.5823156027565313No Hit
ACCGGGTGCTGTAGGCTT321060.5773523791643874No Hit
TGTAAACATCCTTGACTGGAAGCT306400.5509897495046666No Hit
ACTGGACTTGGAGTCAGAAGGC295530.5314425609370567No Hit
TGAGGTAGTAGATTGTATAGTT262000.47114658737017845No Hit
TGTAAACATCCCCGACTGGAAGCT230310.4141594295313962No Hit
CCCATAAAGTAGAAAGCACT229850.4133322255993722No Hit
TTCAAGTAATTCAGGATAGGTT225040.4046825497014693No Hit
CATTGCACTTGTCTCGGTCTGA214970.38657397666781396No Hit
CAACGGAATCCCAAAAGCAGCT202020.36328638771192157No Hit
CAACGGAATCCCAAAAGCAGCTGT182890.3288854937562288No Hit
TTCACAGTGGCTAAGTTCCG167110.3005088023489714No Hit
TTCTCACTACTGCACTTGACTAG157300.28286777936385143No Hit
AACATTCAACGCTGTCGGTGAGTT156050.28061994259204714No Hit
ACCGGGTGCTGTAGGCTTT143560.2581595575681787No Hit
ATCCCGGACGAGCCCCCA142100.25553408421871127No Hit
CCCATAAAGTAGAAAGCACTAC140640.2529086108692439No Hit
TACGCCTGTCTGAGCGTCGCT137500.24726204489847148No Hit
CTCCCACTGCTTCACTTGACTAG132310.23792902662194013No Hit
TCGTTTCCCGGCCAATGCACCA130370.23444038395209985No Hit
TTCACAGTGGCTAAGTTCTG122770.2207735363795298No Hit
CTACGCCTGTCTGAGCGTCGCT122180.21971255742323814No Hit
TGAGGTAGTAGTTTGTACAGTT117340.21100893344281196No Hit
CTTCTCACTACTGCACTTGACTAG117290.21091901997193982No Hit
TGAGGTAGTAGTTTGTGCTGTT116500.2094983871321595No Hit
AACGGAATCCCAAAAGCAGCTG115950.20850933895256563No Hit
ACTGGACTTGGAGTCAGAAGG115790.20822161584577464No Hit
ACCGGGTGCTGTAGGCTTA115690.20804178890403033No Hit
CACCCGGCTGTGTGCACATGTGC112180.20172986324880388No Hit
ACGCCTGTCTGAGCGTCGCT112090.20156801900123397No Hit
CACTAGATTGTGAGCTCCTGGA110480.19867280523915004No Hit
TAGCTTATCAGACTGATGTTGACA106130.19085033327327114No Hit
GCGTTCGCGCTTTCCCCTG103440.1860129885403483No Hit
TATTGCACTTGTCCCGGCCTGTT101970.18336953249670648No Hit
TGAGGTAGTAGGTTGTGTGGTT93190.16758072701155316No Hit
CCCCCCACTGCTAAATTTGACTGG91990.16542280371062104No Hit
AGCAGCATTGTACAGGGCTATG90170.16214995337087398No Hit
ACTGGACTTGGAGTCAGAAGGCA87740.15778015868648645No Hit
TTCAAGTAATCCAGGATAGGC85920.15450730834673942No Hit
ACTGGACTTGGAGTCAGAAGGCT84010.1510726137594225No Hit
CGCGACCTCAGATCAGACGTGGCG83590.15031734060409624No Hit
GCCTGTCTGAGCGTCGCT82720.14875284621092044No Hit
ACCCCCCACTGCTAAATTTGACTG82430.14823134807986188No Hit
CCCCCACTGCTAAATTTGACTGGCTT82320.1480335384439431No Hit
ACCACAGGGTAGAACCACGGAC82230.14787169419637317No Hit
TAGCAGCACGTAAATATTGGC82110.14765590186627997No Hit
CAAAGAATTCTCCTTTTGGGCT81680.14688264601677928No Hit
CGCGACCTCAGATCAGACGTGG77410.13920403560429587No Hit
CACTAGATTGTGAGCTCCTGGAA76840.1381790220363531No Hit
TGTCAGTTTGTCAAATACCCCAA74860.1346184485898151No Hit
TGTAAACATCCTACACTCTCAGCT72140.12972715577436897No Hit
AGCAGCATTGTACAGGGCTATGT69800.12551920533755134No Hit
TATTGCACTTGTCCCGGCCTGTAG68620.1233972474249681No Hit
AATGACACGATCACTCCCGTTG65410.11762480259497471No Hit
TGTAAACATCCCCGACTGGAAGC62930.113165094439715No Hit
AACATTCAACGCTGTCGGTGAGA62790.11291333672127292No Hit
CAAAGTGCTGTTCGTGCAGGT62610.1125896482261331No Hit
TGAGGTAGTAGGTTGTATAGT62520.11242780397856318No Hit
TTCAAGTAATCCAGGATAGGCTT61950.11140279041062043No Hit
TTATAAAGCAATGAGACTGATT61780.11109708460965505No Hit
CCCATAAAGTAGAAAGCACTA61740.11102515383295733No Hit
AATGCACCCGGGCAAGGATTCT60180.10821985354174557No Hit
AACATTCAACGCTGTCGGTGA60130.1081299400708734No Hit
ATCCCACCGCTGCCACCA58410.1050369166728707No Hit
TTCACAGTGGCTAAGTTCTGC58050.10438953968259107No Hit
CAAAGTGCTGTTCGTGCAGGTAG56450.10151230861468158No Hit
TATTGCACTTGTCCCGGCCTGA55820.10037939888169223No Hit
CAACGGAATCCCAAAAGCAGCTGA55630.10003772769237797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)