FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9830276
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG151205215.381582368592703No Hit
CCCCCCACTGCTAAATTTGACTG5904446.006382730250911No Hit
TTCAAGTAATCCAGGATAGGCT4246424.319736292246525No Hit
CCCCCACTGCTAAATTTGACTG2702022.7486715530672794No Hit
TAGCAGCACGTAAATATTGGCG2619812.6650421615832554No Hit
TCGTACGACTCTTAGCGG2615522.6606780928633134No Hit
AACATTCAACGCTGTCGGTGAG2309132.348998135962815No Hit
TGAGGTAGTAGATTGTATAGTT2307312.3471467128695065No Hit
AAGCTGCCAGTTGAAGAACTGT2261902.300952689426014No Hit
TGAGGTAGTAGGTTGTATAGTT1757211.7875489965897193No Hit
TAGCTTATCAGACTGATGTTGAC1704831.7342646330581155No Hit
TAGCTTATCAGACTGATGTTGA1537311.5638523272388283No Hit
TATTGCACTTGTCCCGGCCTGT1285201.3073895381981135No Hit
TAGCTTATCAGACTGATGTTGACT941840.9581012781329843No Hit
TGAGGTAGTAGTTTGTACAGTT837580.8520411837877186No Hit
TATTGCACTTGTCCCGGCCTGTAA773560.7869158505824252No Hit
CATAAAGTAGAAAGCACTACT718900.7313121218570059No Hit
AACATTCAACGCTGTCGGTGAGT707530.7197458138510048No Hit
CAACGGAATCCCAAAAGCAGCTGT679220.6909470293611288No Hit
TATTGCACTTGTCCCGGCCTGTAT659240.6706220659521666No Hit
TATTGCACTTGTCCCGGCCTGTA600620.6109899661006466No Hit
TGTAAACATCCTTGACTGGAAGCT594230.6044896399653479No Hit
TTCAAGTAATTCAGGATAGGTT591290.601498879583849No Hit
CCCATAAAGTAGAAAGCACT580910.5909396643593731No Hit
AGCAGCATTGTACAGGGCTATGA578890.5888847881788873No Hit
CATTGCACTTGTCTCGGTCTGA513920.522793052809504No Hit
CAACGGAATCCCAAAAGCAGCT485290.49366874338014516No Hit
TTCTCACTACTGCACTTGACTAG470650.4787759773988034No Hit
GCCTGTCTGAGCGTCGCT457730.4656329079671822No Hit
TGAGAACTGAATTCCATGGGTT453380.46120780332108685No Hit
CCCCCACTGCTAAATTTGACTGGCTT416470.42366053608260845No Hit
CCCATAAAGTAGAAAGCACTAC378010.3845365074185099No Hit
AACGGAATCCCAAAAGCAGCTG373780.3802334746247206No Hit
TGTAAACATCCCCGACTGGAAGCT315230.32067258335371257No Hit
ACTGGACTTGGAGTCAGAAGGC315000.3204386123034592No Hit
TGAGAACTGAATTCCATAGGCTGT294670.29975760599193757No Hit
TTCACAGTGGCTAAGTTCCGC294660.2997474333375787No Hit
TGAGGTAGTAGATTGTATAGT289150.2941423007858579No Hit
TGATGTAGTAGATTGTATAGTT284010.28891355644541417No Hit
CTCCCACTGCTTCACTTGACTAG278460.283267733276258No Hit
CAACACCAGTCGATGGGCTGT270060.2747227036148324No Hit
TTCACAGTGGCTAAGTTCTGC261790.2663099184600717No Hit
TAGCAGCACGTAAATATTGGC256990.26142704436782854No Hit
CACCCGGCTGTGTGCACATGTGC250820.2551505166284243No Hit
AACCGGGCGGAAACACCA245890.2501353980295161No Hit
TAGCTTATCAGACTGATGTTGACA229430.23339120895486554No Hit
CTTCTCACTACTGCACTTGACTAG227910.23184496549232186No Hit
TGATGTAGTAGGTTGTATAGTT218580.2223538789755242No Hit
TGAGGTAGTAAGTTGTATTGTT217870.22163162051604654No Hit
ACCGGGTGCTGTAGGCTT210870.21451076246485856No Hit
TTCAAGTAATCCAGGATAGGC199120.2025578935932216No Hit
TGAGGTAGTAGTTTGTGCTGTT198310.20173390859015558No Hit
CAAAGAATTCTCCTTTTGGGCT190820.1941145904753844No Hit
TCGTTTCCCGGCCAATGCACCA188150.19139849176157414No Hit
TGTAAACATCCCCGACTGGA185920.18912998983955281No Hit
CCCATAAAGTAGAAAGCACTA183310.18647492705189558No Hit
CATTATTACTTTTGGTACGCG180070.18317898703963142No Hit
TATTGCACTTGTCCCGGCCTGTT177600.18066634141299798No Hit
TGTAAACATCCTACACTCTCAGCT168920.17183647742952485No Hit
ACCGGGTGCTGTAGGCTTT163500.1663228987670336No Hit
TTCACAGTGGCTAAGTTCTG156030.15872392596098014No Hit
TGGCTCAGTTCAGCAGGAAC153570.15622145298870552No Hit
AGCTCGGTCTGAGGCCCCTCAGT151490.1541055408820668No Hit
TTCAAGTAATCCAGGATAGGCTT149530.15211170062773416No Hit
AATGCACCCGGGCAAGGATTCT148860.15143013278569187No Hit
CTCCCACTGCTTCACTTGACTAGCCT144320.14681174770677854No Hit
CAAAGTGCTGTTCGTGCAGGTAG143760.1462420790626835No Hit
CACTAGATTGTGAGCTCCTGGA139080.14148127682274636No Hit
CCCCCCACTGCTAAATTTGACTGGCT134040.13635425902589102No Hit
CCCCCCACTGCTAAATTTGACTGG131520.13379075012746336No Hit
TGTCAGTTTGTCAAATACCCCA121440.12353671453375266No Hit
TGAGGTAGTAGGTTGTATAGT120170.12224478743017998No Hit
ACCCCCCACTGCTAAATTTGACTG118010.12004749408867056No Hit
AGAGGTAGTAGGTTGCATAGTT117990.12002714877995288No Hit
AGCAGCATTGTACAGGGCTATGT116610.11862332247843295No Hit
TAGCTTATCAGACTGATGTTG115840.11784002809280228No Hit
TGAGGTAGTAGATTGTATAGTTA114960.11694483450922437No Hit
ATCACATTGCCAGGGATTTCC114590.11656844629794728No Hit
TGAGGTAGTAGTTTGTGCTGTTA114380.11635482055641164No Hit
TTCACAGTGGCTAAGTTCCG107380.10923396250522366No Hit
CACTAGATTGTGAGCTCCTGGAA106630.10847101342831066No Hit
ACTGGACTTGGAGTCAGAAGGCT105310.10712822305294378No Hit
ACCGGGTGCTGTAGGCTTA104910.1067213168785902No Hit
CAACGGAATCCAAAAAGCAGCTG104600.10640596459346614No Hit
TGATGTAGTAGTTTGTACAGTT102670.10444264230221002No Hit
CAAAGAATTCTCCTTTTGGGCTT102110.103872973658115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)