FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8001940
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5721157.149703696853512No Hit
CAACGGAATCCCAAAAGCAGCTG5019776.273191251121603No Hit
TTCAAGTAATCCAGGATAGGCT3055873.8189114139821094No Hit
AAGCTGCCAGTTGAAGAACTGT2578433.222256102895048No Hit
AACATTCAACGCTGTCGGTGAGT2223182.778301261943979No Hit
TAGCAGCACGTAAATATTGGCG1686252.1073014793912477No Hit
TAGCTTATCAGACTGATGTTGAC1666172.0822075646655684No Hit
AACATTCAACGCTGTCGGTGAG1528561.910236767583861No Hit
CCCCCACTGCTAAATTTGACTG1463911.8294438598639828No Hit
TATTGCACTTGTCCCGGCCTGTAA1352681.6904400682834413No Hit
TAGCTTATCAGACTGATGTTGACT1336231.669882553480781No Hit
TATTGCACTTGTCCCGGCCTGTAT1095981.3696428616060605No Hit
TGTAAACATCCTTGACTGGAAGCT846751.0581808911339001No Hit
TAGCTTATCAGACTGATGTTGA778830.9733014743924598No Hit
TGAGGTAGTAGGTTGTATAGTT771690.9643786381802413No Hit
TATTGCACTTGTCCCGGCCTGT644340.8052297317900409No Hit
AGCAGCATTGTACAGGGCTATGA624040.7798608837356941No Hit
TTAATGCTAATCGTGATAGGGGTT522060.6524167889286848No Hit
TATTGCACTTGTCCCGGCCTGTA516270.6451810435969277No Hit
TGTAAACATCCCCGACTGGAAGCT484570.605565650329795No Hit
TGAGGTAGTAGATTGTATAGTT484470.605440680634946No Hit
CATAAAGTAGAAAGCACTACT475670.594443347488234No Hit
TTCAAGTAATTCAGGATAGGTT469750.5870451415531733No Hit
TCTCCCAACCCTTGTACCAGTGA420630.5256600274433451No Hit
ACTGGACTTGGAGTCAGAAGGC412660.5156999427638798No Hit
CGCCTGTCTGAGCGTCGCT393420.49165577347493233No Hit
CCCCCACTGCTAAATTTGACTGGCTT392610.4906435189466554No Hit
CAACGGAATCCCAAAAGCAGCTGT364680.45573948317533003No Hit
CCCATAAAGTAGAAAGCACTAC348770.4358568047248542No Hit
TCTCCCAACCCTTGTACCAGTG348220.43516947140318474No Hit
TGTAAACATCCTACACTCTCAGCT343980.42987075634158717No Hit
CCCATAAAGTAGAAAGCACT280620.35068995768526134No Hit
CTACGCCTGTCTGAGCGTCGCT255620.31944753397301157No Hit
TAGCTTATCAGACTGATGTTGACA242690.3032889524290359No Hit
TACGCCTGTCTGAGCGTCGCT238170.2976403222218612No Hit
CAAAGAATTCTCCTTTTGGGCT235220.2939537162238157No Hit
TCGTACGACTCTTAGCGG229360.2866304921056644No Hit
AACCGGGCGGAAACACCA226640.2832313164057716No Hit
AACATTCAACGCTGTCGGTGAGTT206790.2584248319782453No Hit
TGAGGTAGTAGTTTGTACAGTT205690.2570501653349063No Hit
TTCAAGTAATCCAGGATAGGCTT203420.254213353261834No Hit
TTCTCACTACTGCACTTGACTAG190050.2375049050605228No Hit
CATTGCACTTGTCTCGGTCTGA187390.23418071117753944No Hit
CAACGGAATCCCAAAAGCAGCT169790.21218604488411563No Hit
CCCCCCACTGCTAAATTTGACTGGCT166350.20788708738131004No Hit
TGTAAACATCCTACACTCAGCT162610.20321322079395748No Hit
AGCTCGGTCTGAGGCCCCTCAGT160210.2002139481175815No Hit
CTTCTCACTACTGCACTTGACTAG156220.19522765729310643No Hit
TATTGCACTTGTCCCGGCCTGTAG148580.1856799726066429No Hit
TTCAAGTAATCCAGGATAGGC144070.18004383936895302No Hit
CAAAGAATTCTCCTTTTGGGCTT142460.17803182728188413No Hit
CACCCGGCTGTGTGCACATGTGC138400.17295805767101477No Hit
AGCAGCATTGTACAGGGCTATGT137930.17237070010522448No Hit
CTCCCACTGCTTCACTTGACTAG135840.1697588334828804No Hit
CCCCCCACTGCTAAATTTGACTGG135450.1692714516729693No Hit
TCGTTTCCCGGCCAATGCACCA134810.1684716456259357No Hit
CACTAGATTGTGAGCTCCTGGAA129070.16129838514160316No Hit
TATTGCACTTGTCCCGGCCTGTT125000.1562121185612489No Hit
ACGCCTGTCTGAGCGTCGCT123620.1544875367723327No Hit
ATCACATTGCCAGGGATTTCCA120410.15047600956767984No Hit
ACCCCCCACTGCTAAATTTGACTG119240.14901386413794654No Hit
TGAGGTAGTAGTTTGTGCTGTT118060.14753922173872835No Hit
TAGCAGCACGTAAATATTGGC115740.14463992481823157No Hit
TATTGCACTTGTCCCGGCCTGTAAT115500.14433999755059398No Hit
AACGGAATCCCAAAAGCAGCTG114980.14369015513737918No Hit
TTATAAAGCAATGAGACTGATT114160.14266540363961738No Hit
GCGTTCGCGCTTTCCCCTG112910.1411032824540049No Hit
ACCGGGTGCTGTAGGCTT112640.14076586427791263No Hit
CACTAGATTGTGAGCTCCTGGA111200.138966300672087No Hit
TCGTTTCCCGGGCGGCGCACCA107820.13474232498619085No Hit
TGTAAACATCCCCGACTGGAAGC107790.13470483407773615No Hit
TTCACAGTGGCTAAGTTCCG106900.13359260379358007No Hit
TTCACAGTGGCTAAGTTCTGC106770.13343014319027635No Hit
TATTGCACTTGTCCCGGCCTGTAAA105250.13153060382857157No Hit
TCTCCCAACCCTTGTACCAGTGT104960.13116819171350946No Hit
CAAAGTGCTGTTCGTGCAGGTAG104950.13115569474402458No Hit
ACCGGGTGCTGTAGGCTTT102850.1285313311521956No Hit
TCTCCCAACCCTTGTACCAGT102150.1276565432882526No Hit
TGAGGTAGTAGGTTGTGTGGTT99710.12460728273393702No Hit
GGAATACCGGGTGCTGTAGGCTTT99010.12373249486999403No Hit
TAGCAGCACATCATGGTTTACA97360.12167049490498555No Hit
TGTCAGTTTGTCAAATACCCCAA97040.12127059188146874No Hit
TGTCAGTTTGTCAAATACCCCA96510.12060825249876905No Hit
ACCGGGTGCTGTAGGCTTA96080.12007088281091834No Hit
TTCAAGTAATCCAGGATAGGCTA93320.11662171923308598No Hit
ATCACATTGCCAGGGATTTCC92860.11604685863678059No Hit
GGGCTACGCCTGTCTGAGCGTCGCT90800.11347248292289121No Hit
CAACACCAGTCGATGGGCTGT88880.11107306478179042No Hit
TATTGCACTTGTCCCGGCCTGTTT88700.11084811933106221No Hit
CGCGACCTCAGATCAGACGTGGCG86930.10863615573223494No Hit
GTCAGGATGGCCGAGCGGTCTAAGG86100.10759890726498825No Hit
CCAAGTCCTTCTGATCGAGGCCC85750.10716151333301674No Hit
TAGCAGCACGTAAATATTGGCGT84910.10611176789628515No Hit
TTAATGCTAATCGTGATAGGGGTTT84800.10597430123195126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)