FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10599201
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6392996.031577285872775No Hit
CCCCCCACTGCTAAATTTGACTG5732635.40854919158529No Hit
AACATTCAACGCTGTCGGTGAGT4552954.295559636995279No Hit
TTCAAGTAATCCAGGATAGGCT4229153.990064911496631No Hit
AAGCTGCCAGTTGAAGAACTGT3415353.2222711881772974No Hit
AACATTCAACGCTGTCGGTGAG2705432.552484852395949No Hit
TAGCAGCACGTAAATATTGGCG2550102.4059360700867924No Hit
CCCCCACTGCTAAATTTGACTG1876361.7702843827567756No Hit
TGAGGTAGTAGGTTGTATAGTT1557341.4692994311552352No Hit
TAGCTTATCAGACTGATGTTGAC1325761.2508112639811246No Hit
TCGTACGACTCTTAGCGG1274531.2024774320253009No Hit
TAGCTTATCAGACTGATGTTGA1241671.1714750951510402No Hit
TATTGCACTTGTCCCGGCCTGT1135521.0713260367456001No Hit
TAGCTTATCAGACTGATGTTGACT1135341.0711562126239516No Hit
TATTGCACTTGTCCCGGCCTGTAA1077091.0161992399238395No Hit
CGCCTGTCTGAGCGTCGCT1076691.015821852986843No Hit
CATAAAGTAGAAAGCACTACT869910.8207316759065142No Hit
GCCTGTCTGAGCGTCGCT867060.8180427939804141No Hit
TATTGCACTTGTCCCGGCCTGTAT850350.8022774546873864No Hit
TGAGGTAGTAGATTGTATAGTT830370.7834269771844123No Hit
TGTAAACATCCTTGACTGGAAGCT769820.7263000295965706No Hit
TTAATGCTAATCGTGATAGGGGTT760390.7174031325568786No Hit
TATTGCACTTGTCCCGGCCTGTA700160.660578094518634No Hit
TTCAAGTAATTCAGGATAGGTT687360.6485017125347466No Hit
CCCATAAAGTAGAAAGCACT642930.606583458507863No Hit
AGCAGCATTGTACAGGGCTATGA641310.6050550414130272No Hit
ACCGGGTGCTGTAGGCTT541210.5106139604296588No Hit
TGTAAACATCCCCGACTGGAAGCT478480.4514302540351863No Hit
ACTGGACTTGGAGTCAGAAGGC434380.40982334423132455No Hit
ACCGGGTGCTGTAGGCTTT413310.38994448732503517No Hit
CAACGGAATCCCAAAAGCAGCTGT410470.3872650400723602No Hit
TGAGGTAGTAGTTTGTACAGTT410440.3872367360520854No Hit
CCCATAAAGTAGAAAGCACTAC378290.35690426099099354No Hit
CATTGCACTTGTCTCGGTCTGA354260.3342327407509302No Hit
AACATTCAACGCTGTCGGTGAGTT347430.3277888588017153No Hit
TACGCCTGTCTGAGCGTCGCT335760.3167785949148431No Hit
CTACGCCTGTCTGAGCGTCGCT326360.3079100018954259No Hit
AACCGGGCGGAAACACCA323120.30485316770575444No Hit
ACCGGGTGCTGTAGGCTTA308910.29144649676895457No Hit
CCCCCACTGCTAAATTTGACTGGCTT302330.2852384816553625No Hit
TCCTGTCTGAGCGTCGCT297680.2808513585127785No Hit
CAACACCAGTCGATGGGCTGT285870.2697090091979575No Hit
TGTAAACATCCTACACTCTCAGCT279420.2636236448388893No Hit
TTCTCACTACTGCACTTGACTAG256170.241688029125969No Hit
ACGCCTGTCTGAGCGTCGCT255090.24066908439607854No Hit
TTCAAGTAATCCAGGATAGGC246000.23209296625283357No Hit
TGAGGTAGTAGTTTGTGCTGTT241190.22755488833545093No Hit
TCTCCCAACCCTTGTACCAGTG240840.22722467476557906No Hit
TTCACAGTGGCTAAGTTCCG234470.22121478779391016No Hit
ATCCCGGACGAGCCCCCA225630.21287453648628799No Hit
TGAGGTAGTAGGTTGTGTGGTT221200.208694976159052No Hit
AGCTCGGTCTGAGGCCCCTCAGT218100.2057702273973293No Hit
CTCCCACTGCTTCACTTGACTAG204030.1924956418884782No Hit
CAAAGAATTCTCCTTTTGGGCT201760.1903539710210232No Hit
CAACGGAATCCCAAAAGCAGCT195410.18436295339620412No Hit
TTCAAGTAATCCAGGATAGGCTT194710.18370252625646027No Hit
CCCCCCACTGCTAAATTTGACTGGCT187810.17719260159327105No Hit
TATTGCACTTGTCCCGGCCTGTT184260.1738432925274273No Hit
TCTCCCAACCCTTGTACCAGTGA181610.17134310406982564No Hit
GCGTTCGCGCTTTCCCCTG180810.1705883301958327No Hit
TGAGGTAGTAGGTTGTATAGT178240.16816361912563033No Hit
GACCCGCGGGCGCTCTCCAGTCCT175900.16595590554420092No Hit
TCGTTTCCCGGCCAATGCACCA175710.1657766467491276No Hit
CACCCGGCTGTGTGCACATGTGC173770.16394632010469468No Hit
TTCACAGTGGCTAAGTTCTG172130.16239903366300912No Hit
TAGCTTATCAGACTGATGTTGACA167320.15786095574562647No Hit
CACTAGATTGTGAGCTCCTGGA165110.15577589291872093No Hit
CTTCTCACTACTGCACTTGACTAG163650.1543984305986838No Hit
TGTCAGTTTGTCAAATACCCCAA161720.1525775386276758No Hit
AACGGAATCCCAAAAGCAGCTG161180.15206806626273056No Hit
CATTATTACTTTTGGTACGCG159580.15055851851474464No Hit
TGATGTAGTAGGTTGTATAGTT158200.1492565335821068No Hit
ATCCCACCGCTGCCACCA154590.1458506164757136No Hit
TAGCAGCACGTAAATATTGGC150810.1422843099210969No Hit
AGCAGCATTGTACAGGGCTATGT148590.14018981242076642No Hit
CCCCCCACTGCTAAATTTGACTGG140810.13284963649618495No Hit
CCTCGACACAAGGGTTTGT140640.13268924704796145No Hit
AGAGGTAGTAGGTTGCATAGTT140380.13244394553891373No Hit
AACATTCAACGCTGTCGGTGA139900.13199108121451794No Hit
CAAAGTGCTGTTCGTGCAGGT139760.1318589957865692No Hit
TTATAAAGCAATGAGACTGATT138990.13113252593285096No Hit
TTCACAGTGGCTAAGTTCTGC138400.13057588020078117No Hit
CACTAGATTGTGAGCTCCTGGAA133200.12566985001982697No Hit
CAAAGTGCTGTTCGTGCAGGTAG133070.1255471992653031No Hit
TCGTTTCCCGGGCGGCGCACCA132920.12540567916392945No Hit
TATTGCACTTGTCCCGGCCTGTAG126050.11892405852101492No Hit
CTCCCACTGCTTCACTTGACTAGCCT125300.11821645801414653No Hit
ACCACAGGGTAGAACCACGGAC122460.11553701076147155No Hit
TGTCAGTTTGTCAAATACCCCA122410.11548983739434698No Hit
AACATTCAACGCTGTCGGTGAGA122010.11511245045735051No Hit
TGAGAACTGAATTCCATGGGTT121220.11436711125678246No Hit
AGCAGCATTGTACAGGGCTATG120870.11403689768691054No Hit
AACATTCATTGCTGTCGGTGGGT120740.11391424693238669No Hit
TGTAAACATCCTACACTCAGCT117700.11104610621121347No Hit
TCTCCCAACCCTTGTACCAGT117120.11049889515256858No Hit
CTCGACACAAGGGTTTGT113850.10741375694262238No Hit
AATGACACGATCACTCCCGTTG112770.10639481221273188No Hit
CCCATAAAGTAGAAAGCACTA111950.10562116899188911No Hit
TATTGCACTTGTCCCGGCCTGA108430.1023001639463201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)