FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7845531
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5887177.503851555745558No Hit
CCCCCCACTGCTAAATTTGACTG5108236.511006074668496No Hit
TTCAAGTAATCCAGGATAGGCT3075633.9202317854584985No Hit
AAGCTGCCAGTTGAAGAACTGT2714613.4600717274586No Hit
AACATTCAACGCTGTCGGTGAGT2600743.314931774535083No Hit
TAGCAGCACGTAAATATTGGCG2193092.795336606279422No Hit
AACATTCAACGCTGTCGGTGAG1707512.176411003920576No Hit
CCCCCACTGCTAAATTTGACTG1433661.8273587855302593No Hit
TAGCTTATCAGACTGATGTTGAC1335321.702013541212188No Hit
TATTGCACTTGTCCCGGCCTGTAA1108261.41260037083532No Hit
TAGCTTATCAGACTGATGTTGACT1101021.4033721873000056No Hit
TGAGGTAGTAGGTTGTATAGTT932851.1890208578616284No Hit
TATTGCACTTGTCCCGGCCTGTAT902841.1507697821855525No Hit
TAGCTTATCAGACTGATGTTGA899681.146742011471244No Hit
TGTAAACATCCTTGACTGGAAGCT833041.0618019353948127No Hit
TATTGCACTTGTCCCGGCCTGT668630.8522431432620686No Hit
AGCAGCATTGTACAGGGCTATGA605460.7717259673054634No Hit
TGAGGTAGTAGATTGTATAGTT604140.7700434808045498No Hit
GCCTGTCTGAGCGTCGCT545430.6952110698434562No Hit
CATAAAGTAGAAAGCACTACT529820.675314392359166No Hit
CGCCTGTCTGAGCGTCGCT512410.6531234151009027No Hit
TATTGCACTTGTCCCGGCCTGTA499880.637152539452078No Hit
TTCAAGTAATTCAGGATAGGTT496630.6330100537490706No Hit
TGTAAACATCCCCGACTGGAAGCT483160.6158410437738376No Hit
CAACGGAATCCCAAAAGCAGCTGT374900.47785165847920297No Hit
CCCCCACTGCTAAATTTGACTGGCTT362470.4620082439289323No Hit
ACTGGACTTGGAGTCAGAAGGC347800.4433097007710505No Hit
TGTAAACATCCTACACTCTCAGCT342360.43637581700970907No Hit
CCCATAAAGTAGAAAGCACTAC317860.40514784786396224No Hit
CCCATAAAGTAGAAAGCACT317630.4048546873372879No Hit
TTCTCACTACTGCACTTGACTAG299320.381516560192038No Hit
TGAGGTAGTAGTTTGTACAGTT292490.3728109671607951No Hit
AACCGGGCGGAAACACCA273640.3487845500833532No Hit
CAAAGAATTCTCCTTTTGGGCT231760.2954038420089093No Hit
AACATTCAACGCTGTCGGTGAGTT227230.28962985424441No Hit
CTTCTCACTACTGCACTTGACTAG219420.2796751424473372No Hit
CATTGCACTTGTCTCGGTCTGA215100.27416882298980144No Hit
TAGCTTATCAGACTGATGTTGACA213090.27160685490886466No Hit
AGCTCGGTCTGAGGCCCCTCAGT191600.24421546482959536No Hit
GACCCGCGGGCGCTCTCCAGTCCT189370.24137308233183963No Hit
ACCGGGTGCTGTAGGCTT177330.22602676606592975No Hit
TCTCCCAACCCTTGTACCAGTG170300.21706625083757874No Hit
TCTCCCAACCCTTGTACCAGTGA170200.21693878973902467No Hit
CCCCCCACTGCTAAATTTGACTGGCT169830.21646718367437462No Hit
TTCAAGTAATCCAGGATAGGCTT169800.2164289453448084No Hit
CTCCCACTGCTTCACTTGACTAG167020.21288552680500528No Hit
TTCAAGTAATCCAGGATAGGC166440.21214625243339172No Hit
TACGCCTGTCTGAGCGTCGCT164590.20978822211014142No Hit
CTACGCCTGTCTGAGCGTCGCT162110.2066271868660005No Hit
TGTAAACATCCTACACTCAGCT155770.19854615321767258No Hit
TCGTACGACTCTTAGCGG154900.19743724166025217No Hit
TAGCAGCACGTAAATATTGGC154520.1969528894857467No Hit
TGAGGTAGTAGTTTGTGCTGTT152420.1942762064161113No Hit
TTCACAGTGGCTAAGTTCCG148570.18936895412177965No Hit
CAACACCAGTCGATGGGCTGT139420.17770626360408237No Hit
AGCAGCATTGTACAGGGCTATGT132990.16951051496705577No Hit
ACCCCCCACTGCTAAATTTGACTG132350.16869476393630972No Hit
CCCCCCACTGCTAAATTTGACTGG131610.16775155180700962No Hit
TATTGCACTTGTCCCGGCCTGTT131560.1676878212577326No Hit
CAACGGAATCCCAAAAGCAGCT129510.16507486873737418No Hit
TGTCAGTTTGTCAAATACCCCAA129490.16504937651766338No Hit
CACCCGGCTGTGTGCACATGTGC129310.16481994654026605No Hit
TGATGTAGTAGGTTGTATAGTT129270.16476896210084443No Hit
ACGCCTGTCTGAGCGTCGCT127280.16223248623961845No Hit
TTCACAGTGGCTAAGTTCTGC127270.16221974012976303No Hit
AACGGAATCCCAAAAGCAGCTG126560.16131476633002914No Hit
CAAAGAATTCTCCTTTTGGGCTT126420.16113632079205348No Hit
TGTAAACATCCCCGACTGGAAGC124880.15917341987432082No Hit
TATTGCACTTGTCCCGGCCTGTAG124700.1589439898969235No Hit
ACCGGGTGCTGTAGGCTTT122920.15667518234266106No Hit
TCGTTTCCCGGCCAATGCACCA120220.1532337326817012No Hit
TGTCAGTTTGTCAAATACCCCA117720.15004720521784948No Hit
GCGTTCGCGCTTTCCCCTG117130.14929518473638048No Hit
GGAATACCGGGTGCTGTAGGCTTT113330.1444516629913259No Hit
ACCGGGTGCTGTAGGCTTA112180.1429858603579541No Hit
TAGCAGCACGTAAATATTGGCGT111850.14256523873272567No Hit
TGAGGTAGTAGGTTGTGTGGTT109690.13981207900395778No Hit
ATCACATTGCCAGGGATTTCCA106340.1355421322023965No Hit
CACTAGATTGTGAGCTCCTGGAA106180.13533819444470999No Hit
GGGCTACGCCTGTCTGAGCGTCGCT99420.12672182418245495No Hit
TTATAAAGCAATGAGACTGATT97690.12451674717746956No Hit
AGAGGTAGTAGGTTGCATAGTT96740.12330586674120592No Hit
CACTAGATTGTGAGCTCCTGGA95990.1223499085020504No Hit
ACTGGACTTGGAGTCAGAAGGCA95510.12173809522899087No Hit
TGAGGTAGTAGGTTGTATAGT95390.121585141910726No Hit
ATCACATTGCCAGGGATTTCC95380.12157239580087059No Hit
GTCAGGATGGCCGAGCGGTCTAAGG94380.12029778481532989No Hit
CATTATTACTTTTGGTACGCG92890.11839861444687427No Hit
TTCACAGTGGCTAAGTTCTG92560.11797799282164585No Hit
CAAAGTGCTGTTCGTGCAGGTAG89660.11428162096357786No Hit
AACATTCAACGCTGTCGGTGAGA88630.11296877164847095No Hit
AGCAGCATTGTACAGGGCTATG87670.11174514510235191No Hit
TATTGCACTTGTCCCGGCCTGTAAA87440.11145198457567754No Hit
GGAATACCGGGTGCTGTAGGCTT86380.11010089693100442No Hit
TATTGCACTTGTCCCGGCCTGTAAT85800.1093616225593908No Hit
TAGCAGCACATCATGGTTTACA83950.10700359223614056No Hit
TGATGTAGTAGATTGTATAGTT82020.10454359303404702No Hit
CTCCCACTGCTTCACTTGACTAGCCT80190.10221105493050757No Hit
TATTGCACTTGTCCCGGCCTGTTT79000.10069426785771415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)