FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6596002
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6493329.844326911968796No Hit
CAACGGAATCCCAAAAGCAGCTG6009749.111185836511268No Hit
TTCAAGTAATCCAGGATAGGCT2632603.9912055818054633No Hit
TAGCAGCACGTAAATATTGGCG2561023.882685299367708No Hit
AACATTCAACGCTGTCGGTGAGT2121683.216615155665508No Hit
CCCCCACTGCTAAATTTGACTG1927572.922330830099809No Hit
AAGCTGCCAGTTGAAGAACTGT1925132.9186316195780413No Hit
TAGCTTATCAGACTGATGTTGACT1660722.5177675810286293No Hit
AACATTCAACGCTGTCGGTGAG1652162.5047900228047233No Hit
TAGCTTATCAGACTGATGTTGAC1614972.448407383745487No Hit
TAGCTTATCAGACTGATGTTGA1180221.7892960008198906No Hit
TATTGCACTTGTCCCGGCCTGTAA1107981.6797751122573947No Hit
TATTGCACTTGTCCCGGCCTGTAT983801.4915095538176004No Hit
TGTAAACATCCTTGACTGGAAGCT839791.2731803295390147No Hit
TGAGGTAGTAGGTTGTATAGTT752571.1409487140846835No Hit
CATAAAGTAGAAAGCACTACT569900.8640082280144852No Hit
TGAGGTAGTAGATTGTATAGTT566940.8595206611520131No Hit
TGTAAACATCCCCGACTGGAAGCT483040.7323223977191031No Hit
CAACGGAATCCCAAAAGCAGCT450770.683398822498841No Hit
TTCAAGTAATTCAGGATAGGTT445580.675530419790655No Hit
AGCAGCATTGTACAGGGCTATGA433940.6578833663179605No Hit
TATTGCACTTGTCCCGGCCTGT417260.6325953206199756No Hit
CAACGGAATCCCAAAAGCAGCTGT388620.5891750790857856No Hit
TATTGCACTTGTCCCGGCCTGTA379200.5748937007599453No Hit
CCCATAAAGTAGAAAGCACTAC343480.5207396844330854No Hit
TGTAAACATCCTACACTCTCAGCT296790.4499543814571311No Hit
ACTGGACTTGGAGTCAGAAGGC289870.4394631778462166No Hit
TTCTCACTACTGCACTTGACTAG275220.4172527540167514No Hit
CTTCTCACTACTGCACTTGACTAG272120.4125529373702434No Hit
TAGCTTATCAGACTGATGTTGACA263110.39889314769765083No Hit
TGAGGTAGTAGTTTGTACAGTT259450.3933443319149994No Hit
CAAAGAATTCTCCTTTTGGGCT243190.36869303556912203No Hit
CCCCCACTGCTAAATTTGACTGGCTT241440.36603991326867397No Hit
CCCATAAAGTAGAAAGCACT207960.3152818934863877No Hit
TGTCAGTTTGTCAAATACCCCAA197950.30010603392782476No Hit
CTCCCACTGCTTCACTTGACTAG195150.29586103824710785No Hit
TAGCAGCACGTAAATATTGGC190650.2890387237602414No Hit
TCGTTTCCCGGCCAATGCACCA186980.28347474727873034No Hit
ACCCCCCACTGCTAAATTTGACTG184920.2803516433136315No Hit
CATTGCACTTGTCTCGGTCTGA182310.27639470091124896No Hit
CCCCCCACTGCTAAATTTGACTGGCT175920.26670701433989863No Hit
CCCCCCACTGCTAAATTTGACTGG163970.2485899792025533No Hit
CACCCGGCTGTGTGCACATGTGC162180.2458762141066664No Hit
TGTAAACATCCCCGACTGGAAGC153930.2333686375474113No Hit
CAAAGAATTCTCCTTTTGGGCTT143740.21791988540937374No Hit
TGTCAGTTTGTCAAATACCCCA142590.21617640504050786No Hit
AACGGAATCCCAAAAGCAGCTG134310.20362334638467364No Hit
AACATTCAACGCTGTCGGTGAGTT132020.2001515463458016No Hit
TTCAAGTAATCCAGGATAGGCTT131790.1998028502720284No Hit
CAACACCAGTCGATGGGCTGT127880.19387501701788448No Hit
TATTGCACTTGTCCCGGCCTGTAG125640.19047902047331094No Hit
CACTAGATTGTGAGCTCCTGGAA117240.17774403343116027No Hit
TGAGAACTGAATTCCATAGGCTGT116250.17624312424404964No Hit
CAACGGAATCCCAAAAGCAGCTGA108940.16516065337760663No Hit
TTCAAGTAATCCAGGATAGGC104980.15915701662916415No Hit
CTCCCACTGCTTCACTTGACTAGCCT104900.15903573103828653No Hit
GGAATACCGGGTGCTGTAGGCTTT104030.15771675023749235No Hit
CACTAGATTGTGAGCTCCTGGA103020.15618551965266234No Hit
AACCGGGCGGAAACACCA102660.155639734493713No Hit
TATTGCACTTGTCCCGGCCTGTAAT102570.1555032882039757No Hit
AGCTCGGTCTGAGGCCCCTCAGT102180.15491202094844725No Hit
TTCACAGTGGCTAAGTTCCGC100000.15160698859703195No Hit
TGAGGTAGTAGTTTGTGCTGTT99890.1514402209095752No Hit
ACTGGACTTGGAGTCAGAAGGCA98180.14884774140456597No Hit
ATCACATTGCCAGGGATTTCC98050.14865065231938984No Hit
ATGCACCTGGGCAAGGATTCTGA97580.14793809947298378No Hit
ACTGGACTTGGAGTCAGAAGGCT96210.14586108372920445No Hit
TGATGTAGTAGGTTGTATAGTT93970.14246508718463094No Hit
TTCACAGTGGCTAAGTTCTGC93580.1418738199291025No Hit
TATTGCACTTGTCCCGGCCTGTT90810.13767430634496472No Hit
TATTGCACTTGTCCCGGCCTGTAAA90450.1371285211860154No Hit
TGAGAACTGAATTCCATGGGTT90270.13685562860654074No Hit
TAGCAGCACGTAAATATTGGCGT87260.1322922582497701No Hit
TGTAAACATCCTACACTCAGCT85180.12913883288695183No Hit
CCCCCCACTGCTAAATTTGACTGT83010.12584896123439623No Hit
AGCAGCATTGTACAGGGCTATGT82840.12559122935378128No Hit
TAGCTTATCAGACTGATGTTGACC81300.12325648172938697No Hit
ATCACATTGCCAGGGATTTCCA79570.12063368082665833No Hit
TATTGCACTTGTCCCGGCCTGTTT77890.11808668341822819No Hit
TTCACAGTGGCTAAGTTCCG77690.11778346944103413No Hit
TTATAAAGCAATGAGACTGATT77420.11737413057182214No Hit
CCCATAAAGTAGAAAGCACTA77370.11729832707752363No Hit
CCCCCCACTGCTAAATTTGACTGTA74120.11237109994812008No Hit
TGTCAGTTTGTCAAATACCCC70350.10665551647801197No Hit
TGATGTAGTAGATTGTATAGTT70120.10630682040423882No Hit
AACATTCAACGCTGTCGGTGAGA69700.10567007105213129No Hit
TGTAAACATCCTTGACTGGAAGC69320.10509396449546256No Hit
AACATTCAACGCTGTCGGTGA69080.10473010772282967No Hit
TAGCAGCACATCATGGTTTACA68520.10388110858668631No Hit
CGCCTGTCTGAGCGTCGCT66500.10081864741702626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)