FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9717493
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8678428.9307190650922No Hit
CCCCCCACTGCTAAATTTGACTG6047716.2235290521948405No Hit
GCCTGTCTGAGCGTCGCT5741025.907922959141828No Hit
TTCAAGTAATCCAGGATAGGCT3700723.80830734840766No Hit
AAGCTGCCAGTTGAAGAACTGT3605093.7098971926195365No Hit
TAGCAGCACGTAAATATTGGCG3261183.3559890395598946No Hit
AACATTCAACGCTGTCGGTGAGT2917323.0021323401004767No Hit
CCCCCACTGCTAAATTTGACTG2321092.3885687388712293No Hit
AACATTCAACGCTGTCGGTGAG2127552.1894021431247754No Hit
TAGCTTATCAGACTGATGTTGAC1963892.0209842188720897No Hit
TAGCTTATCAGACTGATGTTGACT1650381.6983598547485448No Hit
TAGCTTATCAGACTGATGTTGA1495491.5389668919751216No Hit
TGAGGTAGTAGGTTGTATAGTT1307641.3456557159341405No Hit
TATTGCACTTGTCCCGGCCTGTAA1009701.0390540029203006No Hit
TGTAAACATCCTTGACTGGAAGCT944900.972370137030199No Hit
TATTGCACTTGTCCCGGCCTGTAT865570.8907338549150486No Hit
CATAAAGTAGAAAGCACTACT850390.8751125418870896No Hit
TGAGGTAGTAGATTGTATAGTT767660.7899774149567178No Hit
TCCTGTCTGAGCGTCGCT738190.7596506629847842No Hit
TATTGCACTTGTCCCGGCCTGT675850.6954983142256959No Hit
AACCGGGCGGAAACACCA665750.6851046869804794No Hit
AGCAGCATTGTACAGGGCTATGA594550.611834760261726No Hit
CAACGGAATCCCAAAAGCAGCTGT549460.5654339035798637No Hit
TTCAAGTAATTCAGGATAGGTT528290.5436484492450883No Hit
TGTAAACATCCCCGACTGGAAGCT503230.5178599048128977No Hit
ACTGGACTTGGAGTCAGAAGGC488280.5024752783459685No Hit
CCCATAAAGTAGAAAGCACTAC476390.49023961221273843No Hit
TATTGCACTTGTCCCGGCCTGTA463270.4767381875140018No Hit
CAACGGAATCCCAAAAGCAGCT416280.4283820940236335No Hit
CGCCTGTCTGAGCGTCGCT408720.4206023096697883No Hit
TCGTACGACTCTTAGCGG347410.3575099050753111No Hit
CCCATAAAGTAGAAAGCACT345710.3557604826677004No Hit
TGAGGTAGTAGTTTGTACAGTT338330.34816593127466106No Hit
TGTAAACATCCTACACTCTCAGCT323090.3324828739264335No Hit
TTCTCACTACTGCACTTGACTAG287940.2963109929690714No Hit
TTAATGCTAATCGTGATAGGGGTT278630.28673033260739167No Hit
CATTGCACTTGTCTCGGTCTGA274150.28212009002733524No Hit
TAGCTTATCAGACTGATGTTGACA257150.2646258659512284No Hit
TAGCAGCACGTAAATATTGGC255870.26330865378549795No Hit
CAACACCAGTCGATGGGCTGT246810.2539852614249375No Hit
CCCCCACTGCTAAATTTGACTGGCTT237200.2440958794619147No Hit
AACGGAATCCCAAAAGCAGCTG224350.23087230420438687No Hit
CAAAGAATTCTCCTTTTGGGCT222840.2293184054776268No Hit
GGAATACCGGGTGCTGTAGGCTTT218590.22494484945860008No Hit
CACCCGGCTGTGTGCACATGTGC213370.21957309359523078No Hit
TGAGAACTGAATTCCATAGGCTGT213020.21921291839366386No Hit
CTTCTCACTACTGCACTTGACTAG206000.21198883292223625No Hit
CTCCCACTGCTTCACTTGACTAG197140.2028712549625711No Hit
AACATTCAACGCTGTCGGTGAGTT190910.19646013637468016No Hit
TTCAAGTAATCCAGGATAGGCTT185310.19069733314960968No Hit
AGCTCGGTCTGAGGCCCCTCAGT184580.18994611058634156No Hit
TGAGAACTGAATTCCATGGGTT178430.1836173177588088No Hit
TGTCAGTTTGTCAAATACCCCAA165260.17006443945984834No Hit
CCCCCCACTGCTAAATTTGACTGGCT164320.16909711177564007No Hit
TTCAAGTAATCCAGGATAGGC163730.1684899592929987No Hit
TGAGGTAGTAGTTTGTGCTGTT163030.16776960888986492No Hit
TGTAAACATCCCCGACTGGAAGC161550.16624658232323913No Hit
CCCCCCACTGCTAAATTTGACTGG159520.16415756615415109No Hit
TCGTTTCCCGGCCAATGCACCA159330.16396204247330046No Hit
CTACGCCTGTCTGAGCGTCGCT158580.16319023846994282No Hit
GGAATACCGGGTGCTGTAGGCTT152000.15641894468048498No Hit
TGTCAGTTTGTCAAATACCCCA149770.15412411411050156No Hit
TACGCCTGTCTGAGCGTCGCT147480.15176753922024952No Hit
CAAAGAATTCTCCTTTTGGGCTT147400.15168521345989136No Hit
ACCCCCCACTGCTAAATTTGACTG146550.150810502256086No Hit
TTCACAGTGGCTAAGTTCCG146050.15029596625384758No Hit
TTCACAGTGGCTAAGTTCTGC138420.14244414685968904No Hit
TGAGGTAGTAGGTTGTGTGGTT134270.13817349804110998No Hit
TTCACAGTGGCTAAGTTCCGC134150.13805000940057277No Hit
TAGCAGCACGTAAATATTGGCGT127050.13074359816878695No Hit
CAACGGAATCCCAAAAGCAGCTGA126410.13008499208592175No Hit
ATCACATTGCCAGGGATTTCC126160.12982772408480253No Hit
TGAGGTAGTAGGTTGTATAGT121890.12543358662568627No Hit
TGTAAACATCCTACACTCAGCT121480.12501166710385075No Hit
ACTGGACTTGGAGTCAGAAGGCT121060.12457945686197046No Hit
AGCAGCATTGTACAGGGCTATGT120170.12366358277798606No Hit
TATTGCACTTGTCCCGGCCTGTT117820.12124526356746539No Hit
TTATAAAGCAATGAGACTGATT115040.11838444339501969No Hit
CACTAGATTGTGAGCTCCTGGA114980.11832269907475106No Hit
ACTGGACTTGGAGTCAGAAGGCA113430.11672763746781191No Hit
TATTGCACTTGTCCCGGCCTGTAG112790.1160690313849467No Hit
CACTAGATTGTGAGCTCCTGGAA109310.1124878608093672No Hit
AGAGGTAGTAGGTTGCATAGTT108310.11145878880489031No Hit
CACCCGTAGAACCGACCTTGCG106090.10917424895495165No Hit
CAAAGTGCTGTTCGTGCAGGT105310.10837157279145969No Hit
CCCATAAAGTAGAAAGCACTA103170.10616935870187917No Hit
CTCCCACTGCTTCACTTGACTAGCCT100220.10313359628867239No Hit
ACCACAGGGTAGAACCACGGAC99890.10279400252719503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)