FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637959
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4430626.67467213943322No Hit
GCCTGTCTGAGCGTCGCT4240746.388620357552675No Hit
CCCCCCACTGCTAAATTTGACTG3114294.691637896528135No Hit
AACATTCAACGCTGTCGGTGAGT2536183.8207226046439877No Hit
AAGCTGCCAGTTGAAGAACTGT2408943.629037178445965No Hit
TAGCAGCACGTAAATATTGGCG2000113.0131400329529No Hit
TTCAAGTAATCCAGGATAGGCT1877562.828520031533789No Hit
TAGCTTATCAGACTGATGTTGAC1653142.490434183157805No Hit
AACATTCAACGCTGTCGGTGAG1389272.0929174163323396No Hit
TAGCTTATCAGACTGATGTTGACT1035281.5596360266762719No Hit
TAGCTTATCAGACTGATGTTGA969951.4612172205342033No Hit
CCCCCACTGCTAAATTTGACTG965601.4546640013895837No Hit
TATTGCACTTGTCCCGGCCTGTAT787221.1859368218453896No Hit
TATTGCACTTGTCCCGGCCTGTAA778451.1727249294549724No Hit
TATTGCACTTGTCCCGGCCTGT750391.1304528997542769No Hit
TGAGGTAGTAGGTTGTATAGTT630300.9495388567479853No Hit
TCGTACGACTCTTAGCGG604360.910460579825817No Hit
TTAATGCTAATCGTGATAGGGGTT584390.8803760312469541No Hit
TGTAAACATCCTTGACTGGAAGCT576190.8680228365375562No Hit
AGCAGCATTGTACAGGGCTATGA538720.8115747626642467No Hit
ACTGGACTTGGAGTCAGAAGGC434800.6550206170300239No Hit
TATTGCACTTGTCCCGGCCTGTA410400.6182623303337668No Hit
TGAGGTAGTAGATTGTATAGTT397380.5986478675146985No Hit
AACCGGGCGGAAACACCA394240.5939174978332948No Hit
TTCAAGTAATTCAGGATAGGTT390930.5889310253347453No Hit
TGTAAACATCCCCGACTGGAAGCT389140.5862344133189132No Hit
CGCCTGTCTGAGCGTCGCT384210.5788074316216777No Hit
CATAAAGTAGAAAGCACTACT363800.5480600286925544No Hit
CAACGGAATCCCAAAAGCAGCTGT300470.45265419686985114No Hit
TGTAAACATCCTACACTCTCAGCT295840.4456791613205203No Hit
CCCATAAAGTAGAAAGCACTAC288690.4349077781287893No Hit
AACATTCAACGCTGTCGGTGAGTT211850.3191493047787731No Hit
CATTGCACTTGTCTCGGTCTGA210950.3177934663350587No Hit
GGAATACCGGGTGCTGTAGGCTTT209350.31538308687956645No Hit
CTACGCCTGTCTGAGCGTCGCT204520.30810675389829917No Hit
TAGCTTATCAGACTGATGTTGACA196540.29608498636403147No Hit
CAACGGAATCCCAAAAGCAGCT174230.26247525783151116No Hit
TACGCCTGTCTGAGCGTCGCT167610.25250231283441193No Hit
CCCATAAAGTAGAAAGCACT164760.2482088244293163No Hit
AGCTCGGTCTGAGGCCCCTCAGT152250.22936267006168615No Hit
TGAGGTAGTAGTTTGTACAGTT151890.22882033468420038No Hit
GCGTTCGCGCTTTCCCCTG147420.22208633708041886No Hit
GGAATACCGGGTGCTGTAGGCTT142900.21527701511865321No Hit
CACCCGGCTGTGTGCACATGTGC138570.20875392571722723No Hit
CAAAGAATTCTCCTTTTGGGCT135120.20355654501632206No Hit
TTCAAGTAATCCAGGATAGGCTT134930.20327031245598232No Hit
CTCCCACTGCTTCACTTGACTAG132230.19920279712483913No Hit
TGAGGTAGTAGTTTGTGCTGTT122690.18483090962146648No Hit
TCGTTTCCCGGCCAATGCACCA121320.18276702221270122No Hit
TATTGCACTTGTCCCGGCCTGTT119320.1797540478933359No Hit
TTCTCACTACTGCACTTGACTAG118860.17906106379988188No Hit
TAGCAGCACGTAAATATTGGC118570.1786241825235739No Hit
TGTAAACATCCTACACTCAGCT117720.17734366843784363No Hit
GGGCTACGCCTGTCTGAGCGTCGCT117060.17634938691245305No Hit
CCCCCACTGCTAAATTTGACTGGCTT113390.17082057903641767No Hit
TGTAAACATCCCCGACTGGAAGC112360.16926889726194452No Hit
CACTAGATTGTGAGCTCCTGGA111140.16743098292713166No Hit
TATTGCACTTGTCCCGGCCTGTAC110960.1671598152383888No Hit
AGCAGCATTGTACAGGGCTATGT110300.16616553371299822No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT109300.16465904655331556No Hit
TGAGAACTGAATTCCATAGGCTGT108060.16279100247530906No Hit
ACGCCTGTCTGAGCGTCGCT106380.16026010404704216No Hit
TATTGCACTTGTCCCGGCCTGTAG106250.16006426071628344No Hit
TGAGGTAGTAGGTTGTGTGGTT104930.1580756976655023No Hit
CACTAGATTGTGAGCTCCTGGAA102990.15515311257571793No Hit
AACGGAATCCCAAAAGCAGCTG101970.1536164956728416No Hit
CCCCCCACTGCTAAATTTGACTGGCT100950.1520798787699653No Hit
TTCAAGTAATCCAGGATAGGC97560.14697288729864103No Hit
CAAAGAATTCTCCTTTTGGGCTT97170.1463853573063648No Hit
CAAAGTGCTGTTCGTGCAGGTAG95750.1442461455396154No Hit
CTTCTCACTACTGCACTTGACTAG95240.14347783708817727No Hit
ACCACAGGGTAGAACCACGGAC94500.1423630365900121No Hit
CAACACCAGTCGATGGGCTGT92850.1398773327765357No Hit
ACCCCCCACTGCTAAATTTGACTG89130.13427320054251615No Hit
TAGCAGCACGTAAATATTGGCGT88910.13394177336738597No Hit
CCCCCCACTGCTAAATTTGACTGG87890.13240515646450965No Hit
TCGTTTCCCGGGCGGCGCACCA87870.13237502672131599No Hit
TGATGTAGTAGGTTGTATAGTT87170.13132048570953814No Hit
TCCTGTCTGAGCGTCGCT87150.13129035596634447No Hit
GCCTGTCTGAGCGTCGCTT83750.1261682996234234No Hit
TAGCTTATCAGACTGATGTTGACC81650.1230046765880898No Hit
AACATTCAACGCTGTCGGTGAGA80790.12170909763076271No Hit
TTCACAGTGGCTAAGTTCCG80700.12157351378639127No Hit
AGCAGCATTGTACAGGGCTATG78420.11813872306231478No Hit
CCTCGACACAAGGGTTTGT75500.1137397805560414No Hit
TGTCAGTTTGTCAAATACCCCA75210.11330289927973343No Hit
TGAGAACTGAATTCCATGGGTT75010.1130016018477969No Hit
CAAAGTGCTGTTCGTGCAGGT74240.11184160673484124No Hit
TTAATGCTAATCGTGATAGGGGTTT73340.11048576829112684No Hit
AACATTCAACGCTGTCGGTGA72740.10958187599531724No Hit
ATCCCGGACGAGCCCCCA71700.10801512934924726No Hit
TTATAAAGCAATGAGACTGATT71330.10745772910016468No Hit
GTCAGGATGGCCGAGCGGTCTAAGG71110.10712630192503449No Hit
ACGCGACCTCAGATCAGA70410.10607176091325662No Hit
CGCGACCTCAGATCAGACGTGGCG69220.10427904119323425No Hit
TGTCAGTTTGTCAAATACCCCAA68760.10358605709978022No Hit
TTCACCACCTTCTCCACCCAGC68750.10357099222818339No Hit
CGGGCCGCCGGTGAAATACCACTACT68090.10257671070279283No Hit
TACCTGGTTGATCCTGCC67370.10149203994782131No Hit
ACTGGACTTGGAGTCAGAAGGCT67350.10146191020462766No Hit
GAATACCGGGTGCTGTAGGCTTT67170.10119074251588478No Hit
TGAGGTAGTAGGTTGTATAGT66760.10057308278041488No Hit
ACCGGGTGCTGTAGGCTT66710.10049775842243076No Hit
TATTGCACTTGTCCCGGCCTGTTT66610.10034710970646249No Hit
AACATTCATTGCTGTCGGTGGGT66540.1002416556052847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)