FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6899549
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG79740711.557378605471168No Hit
CCCCCCACTGCTAAATTTGACTG4873077.062881936196119No Hit
TTCAAGTAATCCAGGATAGGCT2723773.9477507877688818No Hit
AAGCTGCCAGTTGAAGAACTGT2168963.1436257645246086No Hit
TAGCAGCACGTAAATATTGGCG2111673.0605913516955963No Hit
AACATTCAACGCTGTCGGTGAGT2020512.928466773697817No Hit
AACATTCAACGCTGTCGGTGAG1816372.632592362196428No Hit
CCCCCACTGCTAAATTTGACTG1573062.2799461240147725No Hit
GCCTGTCTGAGCGTCGCT1108301.6063368779611538No Hit
TATTGCACTTGTCCCGGCCTGTAA957341.3875399681921239No Hit
TATTGCACTTGTCCCGGCCTGTAT828391.2006436942472616No Hit
TGAGGTAGTAGGTTGTATAGTT823021.1928605768290073No Hit
TAGCTTATCAGACTGATGTTGACT817561.184947016102067No Hit
TAGCTTATCAGACTGATGTTGA813881.179613334146913No Hit
TAGCTTATCAGACTGATGTTGAC802981.1638152000949629No Hit
TATTGCACTTGTCCCGGCCTGT588290.8526499340754011No Hit
TTAATGCTAATCGTGATAGGGGTT560920.8129806745339441No Hit
CATAAAGTAGAAAGCACTACT551130.7987913412891191No Hit
GGAATACCGGGTGCTGTAGGCTTT545240.7902545514206797No Hit
CAACGGAATCCCAAAAGCAGCTGT510020.7392077366216255No Hit
TGTAAACATCCTTGACTGGAAGCT442460.6412882929014636No Hit
AACCGGGCGGAAACACCA438300.6352589132999853No Hit
TATTGCACTTGTCCCGGCCTGTA437180.6336356187918949No Hit
CCCATAAAGTAGAAAGCACTAC432810.6273018714701497No Hit
TGAGGTAGTAGATTGTATAGTT418970.6072425893344623No Hit
TTCAAGTAATTCAGGATAGGTT373630.5415281491587349No Hit
GGAATACCGGGTGCTGTAGGCTT372040.5392236507052852No Hit
AGCAGCATTGTACAGGGCTATGA334600.4849592342919805No Hit
TCGTACGACTCTTAGCGG314420.4557109457444248No Hit
ACTGGACTTGGAGTCAGAAGGC281580.40811363177506244No Hit
TGAGAACTGAATTCCATAGGCTGT272490.3949388575977937No Hit
TGTAAACATCCCCGACTGGAAGCT255230.3699227297320448No Hit
TGAGAACTGAATTCCATGGGTT251750.36487892179619275No Hit
CCCATAAAGTAGAAAGCACT231190.3350798726119635No Hit
CAACGGAATCCCAAAAGCAGCT221080.3204267409362554No Hit
CCCCCACTGCTAAATTTGACTGGCTT205170.29736726270079394No Hit
CATTGCACTTGTCTCGGTCTGA198040.28703325391268325No Hit
TGTAAACATCCTACACTCTCAGCT192330.2787573506616157No Hit
CACCCGGCTGTGTGCACATGTGC189500.2746556332884946No Hit
TGAGGTAGTAGTTTGTACAGTT185300.268568278883156No Hit
AACGGAATCCCAAAAGCAGCTG173990.25217590309163684No Hit
CAAAGAATTCTCCTTTTGGGCT163580.237087960386976No Hit
TCCTGTCTGAGCGTCGCT162610.23568207139336209No Hit
CTCCCACTGCTTCACTTGACTAG160430.23252244458297203No Hit
TTCTCACTACTGCACTTGACTAG154830.22440597204252047No Hit
CAACACCAGTCGATGGGCTGT153100.22189856177555956No Hit
ACCCCCCACTGCTAAATTTGACTG150710.2184345672449025No Hit
TTCAAGTAATCCAGGATAGGC148280.21491259791038514No Hit
TAGCAGCACGTAAATATTGGC146900.21291246717720244No Hit
AGCTCGGTCTGAGGCCCCTCAGT139930.20281035760453328No Hit
CGCCTGTCTGAGCGTCGCT138340.20050585915108365No Hit
TTCACAGTGGCTAAGTTCCG133230.1930995779579216No Hit
TCGTTTCCCGGCCAATGCACCA129450.1876209589931168No Hit
ATCCCGGACGAGCCCCCA127770.18518601723098133No Hit
AACATTCAACGCTGTCGGTGAGTT127680.1850555739222955No Hit
TAGCTTATCAGACTGATGTTGACA123530.1790406880217823No Hit
CCCATAAAGTAGAAAGCACTA121570.17619992263262424No Hit
CCCCCCACTGCTAAATTTGACTGGCT115730.1677356012690105No Hit
CACTAGATTGTGAGCTCCTGGA112600.16319907286693666No Hit
TCGTTTCCCGGGCGGCGCACCA112530.16309761696018102No Hit
CTTCTCACTACTGCACTTGACTAG112180.1625903374264028No Hit
TATTGCACTTGTCCCGGCCTGTT111400.16145982875112563No Hit
TATTGCACTTGTCCCGGCCTGTAG110820.16061919409515027No Hit
CACTAGATTGTGAGCTCCTGGAA110640.16035830747777863No Hit
CTACGCCTGTCTGAGCGTCGCT108640.1574595672847602No Hit
TGAGGTAGTAGGTTGTGTGGTT101710.1474154325159514No Hit
TGAGGTAGTAGTTTGTGCTGTT101180.14664726636480152No Hit
CCCCCCACTGCTAAATTTGACTGG99250.14384998207853875No Hit
TTCAAGTAATCCAGGATAGGCTT96590.13999465762182425No Hit
ATCACATTGCCAGGGATTTCC96070.13924098517163946No Hit
TGATGTAGTAGGTTGTATAGTT93450.13544363551878535No Hit
GGAATACCGGGTGCTGTAGGCTTA90810.13161729846400105No Hit
TGAATACCGGGTGCTGTAGGCTTT88950.12892147008449392No Hit
TGAGGTAGTAGGTTGTATAGT80670.1169206856853977No Hit
TTAATGCTAATCGTGATAGGGGT80330.11642789985258457No Hit
CCCCCCACTGCTAAATTTGACTGT78000.1130508675277181No Hit
TTCACAGTGGCTAAGTTCTGC76460.11081883757909393No Hit
TGTAAACATCCCCGACTGGAAGC76320.11061592576558266No Hit
ACCGGGTGCTGTAGGCTT75110.10886218794880652No Hit
TATTGCACTTGTCCCGGCCTGTAAA74650.10819547770441228No Hit
TGTCAGTTTGTCAAATACCCCA73440.10644173988763614No Hit
TTCACAGTGGCTAAGTTCTG73020.10583300444710227No Hit
ACTGGACTTGGAGTCAGAAGGCT71860.10415173513515158No Hit
TATTGCACTTGTCCCGGCCTGTAAT69310.1004558413890531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)