FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6743855
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG102304615.170047398705933No Hit
CAACGGAATCCCAAAAGCAGCTG6620949.817737777576772No Hit
CCCCCACTGCTAAATTTGACTG3426255.0805511091208215No Hit
TAGCTTATCAGACTGATGTTGAC2696773.9988552541535958No Hit
TAGCAGCACGTAAATATTGGCG1966732.9163290135983053No Hit
TTCAAGTAATCCAGGATAGGCT1716852.5457991015524506No Hit
TAGCTTATCAGACTGATGTTGACT1708402.5332691761611126No Hit
AAGCTGCCAGTTGAAGAACTGT1699772.5204723411164682No Hit
TAGCTTATCAGACTGATGTTGA1629532.4163182630706026No Hit
AACATTCAACGCTGTCGGTGAGT1340821.9882100074808844No Hit
AACATTCAACGCTGTCGGTGAG1155691.7136934290550434No Hit
TATTGCACTTGTCCCGGCCTGT642270.9523781279401766No Hit
TATTGCACTTGTCCCGGCCTGTAA616940.9148180083943086No Hit
TGTAAACATCCTTGACTGGAAGCT601630.8921158595491748No Hit
TATTGCACTTGTCCCGGCCTGTAT568190.842529977290437No Hit
CATAAAGTAGAAAGCACTACT522530.7748238952349954No Hit
AGCAGCATTGTACAGGGCTATGA444560.6592075304110186No Hit
TATTGCACTTGTCCCGGCCTGTA436170.6467665749041164No Hit
TAGCTTATCAGACTGATGTTGACA430290.6380475262294341No Hit
TGAGGTAGTAGGTTGTATAGTT386960.5737964413529058No Hit
CAACGGAATCCCAAAAGCAGCT354950.5263310080065482No Hit
TCGTACGACTCTTAGCGG350270.5193913570205765No Hit
TGTAAACATCCCCGACTGGAAGCT338250.5015677235053245No Hit
TTCAAGTAATTCAGGATAGGTT336510.49898759685669397No Hit
CATTGCACTTGTCTCGGTCTGA304220.45110697071630396No Hit
CAACGGAATCCCAAAAGCAGCTGT276080.4093800949160384No Hit
ACTGGACTTGGAGTCAGAAGGC268300.39784366656756404No Hit
CAACACCAGTCGATGGGCTGT225840.334882645015351No Hit
CCCATAAAGTAGAAAGCACT221810.32890683444409763No Hit
CTTCTCACTACTGCACTTGACTAG221460.328387843451557No Hit
CTCCCACTGCTTCACTTGACTAG213970.3172814362111878No Hit
CACCCGGCTGTGTGCACATGTGC207560.30777648689065823No Hit
CCCATAAAGTAGAAAGCACTAC205140.30418803488509166No Hit
TGAGGTAGTAGATTGTATAGTT202840.3007775226483962No Hit
TTCTCACTACTGCACTTGACTAG197800.2933040523558113No Hit
CGCCTGTCTGAGCGTCGCT183110.271521258983178No Hit
TAGCAGCACGTAAATATTGGC171800.25475043576707984No Hit
TCGTTTCCCGGCCAATGCACCA163320.2421760254335243No Hit
ACCCCCCACTGCTAAATTTGACTG161190.23901759453606283No Hit
CCCCCCACTGCTAAATTTGACTGG140110.2077595084710451No Hit
CAAAGAATTCTCCTTTTGGGCT138940.2060245957245522No Hit
TGTAAACATCCTACACTCTCAGCT131580.19511095656712668No Hit
TATTGCACTTGTCCCGGCCTGTT122110.18106854314038484No Hit
AACGGAATCCCAAAAGCAGCTG119060.17654590734824518No Hit
CTCCCACTGCTTCACTTGACTAGCCT116770.17315022342562228No Hit
AACATTCAACGCTGTCGGTGAGTT115340.17102977451324206No Hit
TTATAAAGCAATGAGACTGATT113140.16776754541727246No Hit
CCCCCACTGCTAAATTTGACTGGCTT112610.16698164477142524No Hit
TGATGTAGTAGGTTGTATAGTT110210.16342284939400387No Hit
TTCAAGTAATCCAGGATAGGCTT103340.1532357976261352No Hit
CACTAGATTGTGAGCTCCTGGA102700.1522867855254895No Hit
CAAAGAATTCTCCTTTTGGGCTT97670.1448281435469772No Hit
CTACGCCTGTCTGAGCGTCGCT95340.14137314636806395No Hit
TGAGGTAGTAGTTTGTGCTGTT93670.1388968179179416No Hit
AGCAGCATTGTACAGGGCTATGT92800.13760675459362637No Hit
ACTTGACTTGGAGTCAGAAGGC91700.13597564004564155No Hit
CACTAGATTGTGAGCTCCTGGAA91550.1357532153345527No Hit
TTCACAGTGGCTAAGTTCCGC90440.13410727247249532No Hit
TGTAAACATCCCCGACTGGAAGC85110.12620378107180535No Hit
CCCCCCACTGCTAAATTTGACTGGCT83340.12357916948095712No Hit
ACCACAGGGTAGAACCACGGAC83340.12357916948095712No Hit
AGCAGCATTGTACAGGGCTATG80720.11969415119393878No Hit
TGAGGTAGTAGTTTGTACAGTT80500.11936792828434181No Hit
TTCACAGTGGCTAAGTTCTGC78890.11698056971865499No Hit
TGTCAGTTTGTCAAATACCCCAA78460.11634295221353366No Hit
TATTGCACTTGTCCCGGCCTGTAG78030.11570533470841232No Hit
TGTCAGTTTGTCAAATACCCCA77610.11508254551736358No Hit
ATCACATTGCCAGGGATTTCC77550.11499357563292806No Hit
TAGCAGCACGTAAATATTGGCGT76350.11321417794421737No Hit
CAAAGTGCTGTTCGTGCAGGTAG76210.11300658154720113No Hit
CTTCTCACTACTGCACTTGACTA75840.11245793392651532No Hit
TAGCTTATCAGACTGATGTTG75590.1120872260747006No Hit
TTCAAGTAATCCAGGATAGGC74530.11051542478300615No Hit
TAGCTTATCAGACTGATGTTGACC71940.10667489143820559No Hit
TTTAAACATCCTTGACTGGAAGCT71920.10664523481006041No Hit
CCCCCCACTGCTAAATTTGACTGTA71380.1058445058501406No Hit
CTCCCACTGCTTCACTTGACTA71050.10535517148574516No Hit
CGCCCCACTGCTAAATTTGACTG70640.10474721060876901No Hit
ACTGGACTTGGAGTCAGAAGGCT68320.10130704174392836No Hit
CCCATAAAGTAGAAAGCACTA68140.10104013209062176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)