FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10403394
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG230763422.18154959814076No Hit
CAACGGAATCCCAAAAGCAGCTG7100236.824916945373788No Hit
CCCCCACTGCTAAATTTGACTG5777085.553072391567598No Hit
AACATTCAACGCTGTCGGTGAGT3246863.120962255202485No Hit
TAGCAGCACGTAAATATTGGCG3186233.062683197425763No Hit
AAGCTGCCAGTTGAAGAACTGT2622252.5205716519051378No Hit
TAGCTTATCAGACTGATGTTGACT2608192.5070568316455186No Hit
TTCAAGTAATCCAGGATAGGCT2517912.4202774594521745No Hit
AACATTCAACGCTGTCGGTGAG2272872.1847389419260677No Hit
TAGCTTATCAGACTGATGTTGAC2269802.1817879818836046No Hit
TAGCTTATCAGACTGATGTTGA1750981.683085346955042No Hit
TATTGCACTTGTCCCGGCCTGTAT1732891.665696790874209No Hit
TATTGCACTTGTCCCGGCCTGTAA1672871.6080040802069016No Hit
TGTAAACATCCTTGACTGGAAGCT1014010.9746915285530856No Hit
TGAGGTAGTAGGTTGTATAGTT997600.9589178300850664No Hit
TATTGCACTTGTCCCGGCCTGT756080.7267628237477116No Hit
TATTGCACTTGTCCCGGCCTGTA710990.6834211988895162No Hit
TGAGGTAGTAGATTGTATAGTT702780.675529543531659No Hit
GGAATACCGGGTGCTGTAGGCTTT600840.5775422905255727No Hit
CATAAAGTAGAAAGCACTACT585530.5628259393040387No Hit
TGTAAACATCCCCGACTGGAAGCT574890.5525985077562188No Hit
AGCAGCATTGTACAGGGCTATGA530090.5095356380811877No Hit
ACTGGACTTGGAGTCAGAAGGC459410.44159627137067003No Hit
TTCAAGTAATTCAGGATAGGTT455910.43823198467730823No Hit
GGAATACCGGGTGCTGTAGGCTT413910.3978605443569666No Hit
CCCATAAAGTAGAAAGCACTAC370570.35620106284545217No Hit
CAACGGAATCCCAAAAGCAGCTGT369740.3554032462867407No Hit
TTCTCACTACTGCACTTGACTAG356470.34264779359505176No Hit
TAGCTTATCAGACTGATGTTGACA323630.31108117216362274No Hit
GACCCGCGGGCGCTCTCCAGTCCT314860.3026512309348276No Hit
CATTGCACTTGTCTCGGTCTGA294680.283253715085673No Hit
TGAGGTAGTAGTTTGTACAGTT290470.27920695880594354No Hit
CTTCTCACTACTGCACTTGACTAG282350.2714018136773441No Hit
CACCCGGCTGTGTGCACATGTGC268150.25775242194999054No Hit
CTCCCACTGCTTCACTTGACTAG266050.25573384993397347No Hit
TGTCAGTTTGTCAAATACCCCAA260430.25033176672920393No Hit
ACCCCCCACTGCTAAATTTGACTG258700.24866884787791368No Hit
TGTAAACATCCTACACTCTCAGCT254240.24438178540580122No Hit
CCCATAAAGTAGAAAGCACT254120.24426643843345738No Hit
CCCCCCACTGCTAAATTTGACTGG250770.2410463354555254No Hit
AACATTCAACGCTGTCGGTGAGTT240700.2313668020263387No Hit
TATTGCACTTGTCCCGGCCTGTAG239280.23000186285360336No Hit
CAAAGAATTCTCCTTTTGGGCT239010.22974233216582976No Hit
TGAGAACTGAATTCCATGGGTT237040.22784871936985182No Hit
TTAATGCTAATCGTGATAGGGGTT236500.22732965799430455No Hit
CACTAGATTGTGAGCTCCTGGAA210280.202126344537177No Hit
CACTAGATTGTGAGCTCCTGGA198800.1910914841829503No Hit
TGAGGTAGTAGTTTGTGCTGTT198710.19100497395369243No Hit
CCCCCACTGCTAAATTTGACTGGCTT196660.18903446317615194No Hit
CCCCCCACTGCTAAATTTGACTGT192910.18542987029040714No Hit
TAGCAGCACGTAAATATTGGC188980.18165225694614662No Hit
CAACACCAGTCGATGGGCTGT187340.18007584832411425No Hit
CCCCCCACTGCTAAATTTGACTGA185720.17851866419747248No Hit
TGTCAGTTTGTCAAATACCCCA185030.17785541910649544No Hit
AACGGAATCCCAAAAGCAGCTG182390.1753177857149311No Hit
TATTGCACTTGTCCCGGCCTGTT176980.1701175597117633No Hit
TGAGAACTGAATTCCATAGGCTGT172100.1654267828364474No Hit
CAAAGAATTCTCCTTTTGGGCTT171450.16480198673625165No Hit
AGCTCGGTCTGAGGCCCCTCAGT164020.15766008669862933No Hit
CCCCCCACTGCTAAATTTGACTGGCT160480.15425735101448623No Hit
TCGTACGACTCTTAGCGG158780.15262326890628194No Hit
TGTAAACATCCCCGACTGGAAGC155030.14901867602053714No Hit
TTCAAGTAATCCAGGATAGGCTT151900.14601004249190216No Hit
ACTGGACTTGGAGTCAGAAGGCA147740.14201134745064928No Hit
ACTGGACTTGGAGTCAGAAGGCT146370.14069446951639053No Hit
TATTGCACTTGTCCCGGCCTGTTT144710.1390988363989675No Hit
CGCCTGTCTGAGCGTCGCT140840.13537889654087887No Hit
TATTGCACTTGTCCCGGCCTGTAAT139630.1342158145697452No Hit
CCCCCCACTGCTAAATTTGACTGTA132640.12749685343071693No Hit
TCGTTTCCCGGGCGGCGCACCA132110.126987404302865No Hit
CTCCCACTGCTTCACTTGACTAGCCT124530.11970132054981288No Hit
TATTGCACTTGTCCCGGCCTGTAAA119600.11496248243602039No Hit
AGCAGCATTGTACAGGGCTATGT119450.1148182987205906No Hit
GGAATACCGGGTGCTGTAGGCTTA111290.10697470460120995No Hit
GCCTGTCTGAGCGTCGCT110070.10580201038238098No Hit
ACTGGACTTGGAGTCAGAAGG109340.10510031630062266No Hit
ATCCCGGACGAGCCCCCA109210.10497535708058352No Hit
TTATAAAGCAATGAGACTGATT108280.10408141804491881No Hit
CAACGGAATCCCAAAAGCAGCT107860.10367770364171539No Hit
TTCAAGTAATCCAGGATAGGC107210.10305290754151963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)