FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7840110
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG95571412.190058557851867No Hit
CAACGGAATCCCAAAAGCAGCTG5740807.322346242590984No Hit
CCCCCACTGCTAAATTTGACTG2800343.5718121301869488No Hit
TAGCAGCACGTAAATATTGGCG2508803.1999551026707533No Hit
TTCAAGTAATCCAGGATAGGCT2507763.198628590670284No Hit
AAGCTGCCAGTTGAAGAACTGT2346112.992445259058865No Hit
TAGCTTATCAGACTGATGTTGACT2324612.965022174433777No Hit
AACATTCAACGCTGTCGGTGAGT2118872.7026023869563054No Hit
TAGCTTATCAGACTGATGTTGAC2032632.592603930301998No Hit
TAGCTTATCAGACTGATGTTGA1748252.229879427712111No Hit
AACATTCAACGCTGTCGGTGAG1540741.9652020188492252No Hit
TATTGCACTTGTCCCGGCCTGTAA1329621.695920082753941No Hit
TATTGCACTTGTCCCGGCCTGTAT1251341.596074544872457No Hit
TGTAAACATCCTTGACTGGAAGCT953611.2163222199688524No Hit
CATAAAGTAGAAAGCACTACT877031.1186450190112127No Hit
TGAGGTAGTAGGTTGTATAGTT764250.9747949964987737No Hit
TGAGGTAGTAGATTGTATAGTT590140.7527190307278853No Hit
TATTGCACTTGTCCCGGCCTGT576820.735729473183412No Hit
TTCAAGTAATTCAGGATAGGTT572790.7305892391815931No Hit
TCGTACGACTCTTAGCGG522330.6662278973126653No Hit
TATTGCACTTGTCCCGGCCTGTA486430.6204377234503088No Hit
TGTAAACATCCCCGACTGGAAGCT477560.6091241066770747No Hit
AGCAGCATTGTACAGGGCTATGA400240.5105030414114088No Hit
TTAATGCTAATCGTGATAGGGGTT379560.4841258604789984No Hit
CAACGGAATCCCAAAAGCAGCTGT355870.45390944769907565No Hit
CAAAGAATTCTCCTTTTGGGCT320140.40833610752910354No Hit
CCCATAAAGTAGAAAGCACTAC318680.40647388875921386No Hit
TAGCTTATCAGACTGATGTTGACA315270.402124459988444No Hit
ACTGGACTTGGAGTCAGAAGGC272610.3477119581230365No Hit
TTCTCACTACTGCACTTGACTAG270110.34452322735267743No Hit
CATTGCACTTGTCTCGGTCTGA267140.34073501519749083No Hit
CCCATAAAGTAGAAAGCACT266680.34014828873574476No Hit
TGAGGTAGTAGTTTGTACAGTT255900.3263984816539564No Hit
TGTAAACATCCTACACTCTCAGCT255190.3254928821151744No Hit
CAACGGAATCCCAAAAGCAGCT248300.3167047401120648No Hit
TGTCAGTTTGTCAAATACCCCAA247210.3153144534961882No Hit
CACCCGGCTGTGTGCACATGTGC236340.30144985210666686No Hit
CTTCTCACTACTGCACTTGACTAG225030.28702403410156235No Hit
TGAGAACTGAATTCCATAGGCTGT222630.2839628525620176No Hit
TGAGAACTGAATTCCATGGGTT212350.27085079163430104No Hit
CAAAGAATTCTCCTTTTGGGCTT205630.2622794833235758No Hit
CAACACCAGTCGATGGGCTGT203890.26006012670740586No Hit
TGTCAGTTTGTCAAATACCCCA193370.24664194762573483No Hit
TCGTTTCCCGGCCAATGCACCA180930.230774823312428No Hit
TAGCAGCACGTAAATATTGGC174930.2231218694635662No Hit
ACCCCCCACTGCTAAATTTGACTG168990.21554544515319302No Hit
TATTGCACTTGTCCCGGCCTGTAG159780.20379816099519013No Hit
CCCCCACTGCTAAATTTGACTGGCTT151890.19373452668393684No Hit
CACTAGATTGTGAGCTCCTGGAA148820.18981876529793587No Hit
AACATTCAACGCTGTCGGTGAGTT148390.18927030360543412No Hit
TGAGGTAGTAGTTTGTGCTGTT143990.1836581374496021No Hit
TTCAAGTAATCCAGGATAGGCTT139310.1776888334474899No Hit
TGTAAACATCCCCGACTGGAAGC137310.17513784883120262No Hit
CACTAGATTGTGAGCTCCTGGA129070.16462779221209908No Hit
TATTGCACTTGTCCCGGCCTGTAAT127040.1620385428265675No Hit
AACGGAATCCCAAAAGCAGCTG125850.16052070697987655No Hit
TTATAAAGCAATGAGACTGATT125610.1602145888259221No Hit
GCCTGTCTGAGCGTCGCT123140.1570641228248073No Hit
TATTGCACTTGTCCCGGCCTGTT121010.15434732420846137No Hit
TATTGCACTTGTCCCGGCCTGTAAA119810.15281673343868898No Hit
CTCCCACTGCTTCACTTGACTAG117830.1502912586685646No Hit
CCCCCCACTGCTAAATTTGACTGG111950.14279136389668004No Hit
CGCCTGTCTGAGCGTCGCT109440.1395898782032395No Hit
TATTGCACTTGTCCCGGCCTGTTT109420.1395643683570766No Hit
TTCAAGTAATCCAGGATAGGC106390.1356996266634014No Hit
TACGCCTGTCTGAGCGTCGCT106200.1354572831248541No Hit
GACCCGCGGGCGCTCTCCAGTCCT105000.13392669235508176No Hit
TTCACAGTGGCTAAGTTCCGC101980.13007470558448797No Hit
ATCCCGGACGAGCCCCCA99850.12735790696814203No Hit
AGCAGCATTGTACAGGGCTATGT98640.12581456127528823No Hit
CACCCGTAGAACCGACCTTGCG98580.1257380317367996No Hit
CCCATAAAGTAGAAAGCACTA98290.12536813896743795No Hit
CCCCCCACTGCTAAATTTGACTGTA97690.12460284358255178No Hit
ACTGGACTTGGAGTCAGAAGGCA97400.12423295081319012No Hit
ACTGGACTTGGAGTCAGAAGGCT94080.11999831635015326No Hit
ATGCACCTGGGCAAGGATTCTGA93260.11895241265747547No Hit
CCCCCCACTGCTAAATTTGACTGT92950.11855701004195095No Hit
TTCACAGTGGCTAAGTTCCG92820.11839119604189227No Hit
TCGTTTCCCGGGCGGCGCACCA92530.11802130327253063No Hit
CTACGCCTGTCTGAGCGTCGCT91850.11715396850299294No Hit
CCCCCCACTGCTAAATTTGACTGGCT91340.11650346742583968No Hit
TGTCAGTTTGTCAAATACCCC88250.11256219619367586No Hit
TCAGTGCACTACAGAACTTTGT87110.11110813496239211No Hit
TAGCAGCACGTAAATATTGGCGT86740.11063620280837896No Hit
TGATGTAGTAGGTTGTATAGTT86320.11010049603895865No Hit
ATCACATTGCCAGGGATTTCC85020.10844235603837192No Hit
TAGCTTATCAGACTGATGTTGACC84490.10776634511505578No Hit
TAGCAGCACATCATGGTTTACA84010.10715410880714683No Hit
TTCACAGTGGCTAAGTTCTGC83790.10687350049935523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)