FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13703379
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG186024013.575045979535414No Hit
CAACGGAATCCCAAAAGCAGCTG10101247.371349796280173No Hit
CCCCCACTGCTAAATTTGACTG6114464.462008968736835No Hit
TTCAAGTAATCCAGGATAGGCT4508503.290064443229659No Hit
TAGCAGCACGTAAATATTGGCG4032432.942653779042381No Hit
AACATTCAACGCTGTCGGTGAGT3784352.7616181381249105No Hit
AAGCTGCCAGTTGAAGAACTGT3305052.4118503910604825No Hit
TAGCTTATCAGACTGATGTTGACT3282002.3950297222312833No Hit
AACATTCAACGCTGTCGGTGAG3154452.301950489729577No Hit
TAGCTTATCAGACTGATGTTGAC2947412.15086366654531No Hit
TAGCTTATCAGACTGATGTTGA2895072.112668707477185No Hit
TATTGCACTTGTCCCGGCCTGTAA1937181.4136513337330887No Hit
TATTGCACTTGTCCCGGCCTGTAT1852681.3519877104763722No Hit
GCCTGTCTGAGCGTCGCT1530921.1171843090671285No Hit
CATAAAGTAGAAAGCACTACT1521431.1102590098398357No Hit
TCGTACGACTCTTAGCGG1277150.931996407601366No Hit
TGAGGTAGTAGGTTGTATAGTT1209240.8824392874195482No Hit
TGTAAACATCCTTGACTGGAAGCT1197900.8741639562037946No Hit
TATTGCACTTGTCCCGGCCTGT981660.7163634604282637No Hit
TATTGCACTTGTCCCGGCCTGTA920410.6716664554049042No Hit
TTCAAGTAATTCAGGATAGGTT886750.6471031706851281No Hit
TGAGGTAGTAGATTGTATAGTT823830.6011874881370499No Hit
TTAATGCTAATCGTGATAGGGGTT680230.4963958159516715No Hit
TGTAAACATCCCCGACTGGAAGCT586230.4277995959974543No Hit
CCCATAAAGTAGAAAGCACT559660.4084102176550762No Hit
AGCAGCATTGTACAGGGCTATGA554570.4046958053192574No Hit
CAACGGAATCCCAAAAGCAGCTGT523230.3818255336877131No Hit
CCCATAAAGTAGAAAGCACTAC515060.3758635005278625No Hit
CATTGCACTTGTCTCGGTCTGA494180.36062638273377684No Hit
TCCTGTCTGAGCGTCGCT479000.34954882295819156No Hit
TTCTCACTACTGCACTTGACTAG444240.324182816515547No Hit
CAACGGAATCCCAAAAGCAGCT444070.3240587595220128No Hit
CAAAGAATTCTCCTTTTGGGCT436830.3187753910914965No Hit
CAACACCAGTCGATGGGCTGT434040.3167393969034937No Hit
ACTGGACTTGGAGTCAGAAGGC418900.30569102700873996No Hit
TGTCAGTTTGTCAAATACCCCAA409720.298991949357892No Hit
TAGCTTATCAGACTGATGTTGACA406320.29651080948720754No Hit
CACCCGGCTGTGTGCACATGTGC387070.28246317933700876No Hit
TGAGGTAGTAGTTTGTACAGTT365120.2664452322306783No Hit
TGAGAACTGAATTCCATGGGTT355000.2590601923802881No Hit
TGTAAACATCCTACACTCTCAGCT344510.2514051461322058No Hit
CTTCTCACTACTGCACTTGACTAG342030.24959537352064773No Hit
TGAGAACTGAATTCCATAGGCTGT329110.24016704201204683No Hit
TAGCAGCACGTAAATATTGGC314180.22927191899165894No Hit
TCGTTTCCCGGCCAATGCACCA311850.22757160843321927No Hit
TGTCAGTTTGTCAAATACCCCA298420.2177711059440157No Hit
TACGCCTGTCTGAGCGTCGCT287160.20955415448992545No Hit
ACCCCCCACTGCTAAATTTGACTG275660.2011620637508457No Hit
AACATTCAACGCTGTCGGTGAGTT268870.1962070814796847No Hit
TATTGCACTTGTCCCGGCCTGTAG243160.17744528557518552No Hit
AACGGAATCCCAAAAGCAGCTG236050.17225678425737184No Hit
CAAAGAATTCTCCTTTTGGGCTT235580.17191380315760077No Hit
TTCACAGTGGCTAAGTTCCG231660.16905319483610576No Hit
CACTAGATTGTGAGCTCCTGGAA228410.16668151701853973No Hit
GACCCGCGGGCGCTCTCCAGTCCT219420.16012109130164173No Hit
CACTAGATTGTGAGCTCCTGGA216990.15834780604112314No Hit
TTATAAAGCAATGAGACTGATT214510.15653803342956507No Hit
CCCATAAAGTAGAAAGCACTA211060.15402040620784116No Hit
TATTGCACTTGTCCCGGCCTGTT209970.15322498195518053No Hit
CTCCCACTGCTTCACTTGACTAG206700.15083870919719872No Hit
CCCCCACTGCTAAATTTGACTGGCTT205530.14998490518287497No Hit
TTCAAGTAATCCAGGATAGGC199350.14547506859439557No Hit
TGAGGTAGTAGTTTGTGCTGTT197940.14444612529508233No Hit
TTCAAGTAATCCAGGATAGGCTT192900.14076820031030304No Hit
CTACGCCTGTCTGAGCGTCGCT191630.1398414215939003No Hit
CCCCCCACTGCTAAATTTGACTGG185590.13543374958833146No Hit
CGTTCGCGCTTTCCCCTG175350.12796114009544654No Hit
TTCACAGTGGCTAAGTTCTG174870.12761086152546755No Hit
CGCCTGTCTGAGCGTCGCT172070.12556756986725684No Hit
CGCCCCACTGCTAAATTTGACTG169610.12377239219611455No Hit
ATCCCGGACGAGCCCCCA162870.11885389727599302No Hit
TATTGCACTTGTCCCGGCCTGTAAA160190.11689817526027704No Hit
TGTAAACATCCCCGACTGGAAGC160060.1168033081475744No Hit
TATTGCACTTGTCCCGGCCTGTAAT159870.11666465621362439No Hit
ATCACATTGCCAGGGATTTCC158830.11590571931200326No Hit
TCGTTTCCCGGGCGGCGCACCA158340.1155481432718164No Hit
ACTGGACTTGGAGTCAGAAGGCT158050.11533651663578742No Hit
CCCCCCACTGCTAAATTTGACTGT157970.11527813687412425No Hit
CCCCCCACTGCTAAATTTGACTGTA156500.11420540875356362No Hit
TTCACAGTGGCTAAGTTCCGC148840.10861554657431573No Hit
ACTGGACTTGGAGTCAGAAGGCA147940.10795877425560513No Hit
AACATTCAACGCTGTCGGTGA147450.10760119821541826No Hit
TAGCAGCACATCATGGTTTACA146190.10668171696922343No Hit
TCAGTGCACTACAGAACTTTGT142870.10425895686020215No Hit
TTTAAACATCCTTGACTGGAAGCT142410.10392327323063895No Hit
CACCCGTAGAACCGACCTTGCG141340.10314244391839414No Hit
TTCACAGTGGCTAAGTTCTGC141330.10313514644818625No Hit
GCCCCCACTGCTAAATTTGACTG139690.10193836133409138No Hit
TATTGCACTTGTCCCGGCCTGTTT138210.10085833574332286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)