FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7583939
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG103757313.68118862770389No Hit
CAACGGAATCCCAAAAGCAGCTG6063007.994526327281905No Hit
CCCCCACTGCTAAATTTGACTG3125024.120576391766864No Hit
TTCAAGTAATCCAGGATAGGCT2610003.4414833769100728No Hit
AACATTCAACGCTGTCGGTGAGT2412903.181592046033071No Hit
TAGCAGCACGTAAATATTGGCG2369903.1248932777544756No Hit
AAGCTGCCAGTTGAAGAACTGT2200762.901869332018625No Hit
TAGCTTATCAGACTGATGTTGACT1776782.3428194767916777No Hit
AACATTCAACGCTGTCGGTGAG1763412.325190115585054No Hit
TAGCTTATCAGACTGATGTTGA1488661.9629113578049613No Hit
TAGCTTATCAGACTGATGTTGAC1485841.9591929734666904No Hit
TATTGCACTTGTCCCGGCCTGTAA1223671.613501901848103No Hit
TGAGGTAGTAGGTTGTATAGTT1197101.5784673373559572No Hit
TATTGCACTTGTCCCGGCCTGTAT1128301.4877493081102051No Hit
TGTAAACATCCTTGACTGGAAGCT748470.9869145835693035No Hit
TGAGGTAGTAGATTGTATAGTT748280.9866640541280725No Hit
TATTGCACTTGTCCCGGCCTGT602580.7945475299840887No Hit
CATAAAGTAGAAAGCACTACT600230.7914488763688632No Hit
TATTGCACTTGTCCCGGCCTGTA531520.7008495189636942No Hit
TTCAAGTAATTCAGGATAGGTT490140.646286843815595No Hit
AGCAGCATTGTACAGGGCTATGA444560.5861861494402842No Hit
TGTAAACATCCCCGACTGGAAGCT443150.5843269572711489No Hit
CCCATAAAGTAGAAAGCACTAC376320.49620652275816035No Hit
ACTGGACTTGGAGTCAGAAGGC366930.483825094057323No Hit
CAACGGAATCCCAAAAGCAGCTGT345380.45540978111770153No Hit
TCGTACGACTCTTAGCGG334240.44072084440552595No Hit
TGAGGTAGTAGTTTGTACAGTT307180.40504017767020545No Hit
CAACGGAATCCCAAAAGCAGCT302540.3989219850001431No Hit
TTCTCACTACTGCACTTGACTAG300830.39666722002906407No Hit
CCCATAAAGTAGAAAGCACT281050.3705857866209103No Hit
CTTCTCACTACTGCACTTGACTAG279460.3684892507706088No Hit
CATTGCACTTGTCTCGGTCTGA266770.35175652124839085No Hit
CACCCGGCTGTGTGCACATGTGC241890.3189503502071945No Hit
TAGCTTATCAGACTGATGTTGACA221970.2926843161581337No Hit
CTCCCACTGCTTCACTTGACTAG218510.28812204317571644No Hit
CAAAGAATTCTCCTTTTGGGCT212500.28019740137677795No Hit
CAACACCAGTCGATGGGCTGT211170.27844369528816093No Hit
ACCCCCCACTGCTAAATTTGACTG208160.2744747815086593No Hit
TGTAAACATCCTACACTCTCAGCT205480.27094099781129566No Hit
GACCCGCGGGCGCTCTCCAGTCCT188420.2484460911407647No Hit
AACATTCAACGCTGTCGGTGAGTT184430.24318497287491367No Hit
CCCCCACTGCTAAATTTGACTGGCTT183930.2425256848716742No Hit
TGAGGTAGTAGTTTGTGCTGTT176460.2326759221032764No Hit
CGCCTGTCTGAGCGTCGCT167030.22024175036217986No Hit
AACGGAATCCCAAAAGCAGCTG163230.21523116153755983No Hit
TCGTTTCCCGGCCAATGCACCA162910.21480921721548657No Hit
TAGCAGCACGTAAATATTGGC146890.1936856295916937No Hit
CAAAGAATTCTCCTTTTGGGCTT140420.18515444282977486No Hit
TTCAAGTAATCCAGGATAGGCTT139130.18345347978141702No Hit
TATTGCACTTGTCCCGGCCTGTAG138690.18287330633856627No Hit
CCCCCCACTGCTAAATTTGACTGG134100.17682104246882788No Hit
TATTGCACTTGTCCCGGCCTGTT127910.16865905698872313No Hit
TTCAAGTAATCCAGGATAGGC126450.16673393601926387No Hit
TGTAAACATCCCCGACTGGAAGC126250.16647022081796808No Hit
CACTAGATTGTGAGCTCCTGGAA125950.1660746480160244No Hit
TTCACAGTGGCTAAGTTCCG123030.16222440607710584No Hit
CACTAGATTGTGAGCTCCTGGA121100.15967955438460146No Hit
CCCCCCACTGCTAAATTTGACTGGCT121090.15966636862453668No Hit
ACTGGACTTGGAGTCAGAAGGCT119350.1573720463732633No Hit
ACTGGACTTGGAGTCAGAAGGCA113560.14973749129575015No Hit
CTCCCACTGCTTCACTTGACTAGCCT111640.14720582536331053No Hit
AGCAGCATTGTACAGGGCTATGT108860.14354018406529906No Hit
TGTCAGTTTGTCAAATACCCCAA108570.14315779702342013No Hit
TTATAAAGCAATGAGACTGATT108290.14278859574160604No Hit
TGAGGTAGTAGGTTGTATAGT107410.14162824885590458No Hit
CCCCCCACTGCTAAATTTGACTGTA105850.1395712702857974No Hit
TGTCAGTTTGTCAAATACCCCA102100.1346266102615013No Hit
TTCACAGTGGCTAAGTTCTG100530.13255644593132937No Hit
CCCATAAAGTAGAAAGCACTA100480.1324905171310054No Hit
ACTGGACTTGGAGTCAGAAGG96850.1277040862274868No Hit
TATTGCACTTGTCCCGGCCTGTAAA96650.12744037102619102No Hit
TATTGCACTTGTCCCGGCCTGTAAT93420.123181370525264No Hit
TATTGCACTTGTCCCGGCCTGTTT91040.12004315962984406No Hit
TTCACAGTGGCTAAGTTCTGC90160.1188828127441426No Hit
TGAGGTAGTAGGTTGTGTGGTT88790.11707636361526642No Hit
ATCCCGGACGAGCCCCCA88440.11661486201299878No Hit
CCCCCCACTGCTAAATTTGACTGA86090.11351620839777324No Hit
AGCTCGGTCTGAGGCCCCTCAGT85110.11222400391142386No Hit
CTACGCCTGTCTGAGCGTCGCT84260.11110321430591674No Hit
CCCCCCACTGCTAAATTTGACTGT83160.10965278069878989No Hit
CAACGGAATCCCAAAAGCAGCTGA82060.10820234709166304No Hit
ATGCACCTGGGCAAGGATTCTGA76200.10047549169369636No Hit
TAGCAGCACGTAAATATTGGCGT76140.10039637713330765No Hit
AACATTCAACGCTGTCGGTGA76120.10037000561317806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)