FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10035071
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG111572911.118297020519337No Hit
CAACGGAATCCCAAAAGCAGCTG104357310.399258759604193No Hit
AACATTCAACGCTGTCGGTGAGT3838693.825274380221126No Hit
TAGCAGCACGTAAATATTGGCG3822063.80870249946413No Hit
TTCAAGTAATCCAGGATAGGCT3435843.423832277818463No Hit
AAGCTGCCAGTTGAAGAACTGT3404103.392203204142751No Hit
CCCCCACTGCTAAATTTGACTG3402573.3906785512529005No Hit
TAGCTTATCAGACTGATGTTGACT2684442.6750583030254593No Hit
AACATTCAACGCTGTCGGTGAG2633142.6239375884834297No Hit
TAGCTTATCAGACTGATGTTGAC2261452.2535465867655544No Hit
TAGCTTATCAGACTGATGTTGA1588851.5832972183256102No Hit
TATTGCACTTGTCCCGGCCTGTAA1449441.444374434421042No Hit
TGAGGTAGTAGGTTGTATAGTT1318891.314280686205409No Hit
TATTGCACTTGTCCCGGCCTGTAT1208561.2043362722595585No Hit
TGTAAACATCCTTGACTGGAAGCT1063611.0598928497865137No Hit
TATTGCACTTGTCCCGGCCTGT785270.7825256044526241No Hit
CATAAAGTAGAAAGCACTACT773080.7703782065916623No Hit
TGAGGTAGTAGATTGTATAGTT693150.6907275494114591No Hit
AGCAGCATTGTACAGGGCTATGA652540.6502594749952442No Hit
TGTAAACATCCCCGACTGGAAGCT606520.6044003076809322No Hit
TTCAAGTAATTCAGGATAGGTT572300.5702999012164438No Hit
TATTGCACTTGTCCCGGCCTGTA565640.5636631768723909No Hit
ACTGGACTTGGAGTCAGAAGGC555640.5536981253047437No Hit
CAACGGAATCCCAAAAGCAGCTGT540020.538132714756079No Hit
CACCCGGCTGTGTGCACATGTGC451700.45012137931061974No Hit
CGCCTGTCTGAGCGTCGCT414730.41328058366502834No Hit
CAACGGAATCCCAAAAGCAGCT407520.40609578148475484No Hit
CAACACCAGTCGATGGGCTGT381870.38053542421374No Hit
CCCATAAAGTAGAAAGCACTAC376240.3749251001811547No Hit
CATTGCACTTGTCTCGGTCTGA343890.3426881583598163No Hit
CCCATAAAGTAGAAAGCACT340840.3396488176316839No Hit
TGTCAGTTTGTCAAATACCCCAA338950.3377654228853986No Hit
TAGCTTATCAGACTGATGTTGACA299680.29863266537924843No Hit
TGAGGTAGTAGTTTGTACAGTT295150.2941184970191043No Hit
CAAAGAATTCTCCTTTTGGGCT289540.28852810308965426No Hit
TGTAAACATCCTACACTCTCAGCT281980.2809945241045131No Hit
TTCTCACTACTGCACTTGACTAG268130.2671929276833218No Hit
CTTCTCACTACTGCACTTGACTAG264990.2640639014910806No Hit
AACGGAATCCCAAAAGCAGCTG243960.24310739804431877No Hit
AACATTCAACGCTGTCGGTGAGTT243100.24225040360950112No Hit
TAGCAGCACGTAAATATTGGC241010.24016770783186286No Hit
CTCCCACTGCTTCACTTGACTAG214120.21337168416645982No Hit
TGAGGTAGTAGTTTGTGCTGTT203140.2024300575451833No Hit
CAAAGAATTCTCCTTTTGGGCTT202420.2017125738323127No Hit
CACTAGATTGTGAGCTCCTGGA194420.193740532578195No Hit
TGTCAGTTTGTCAAATACCCCA186220.1855691902927244No Hit
TGTAAACATCCCCGACTGGAAGC183410.18276901080221555No Hit
ACTGGACTTGGAGTCAGAAGGCA181210.18057669945733318No Hit
TTCAAGTAATCCAGGATAGGCTT178680.17805554141071847No Hit
CACTAGATTGTGAGCTCCTGGAA178310.17768683450271552No Hit
ACTGGACTTGGAGTCAGAAGGCT176810.17619207676756846No Hit
CCCCCACTGCTAAATTTGACTGGCTT171900.17129923644785375No Hit
TATTGCACTTGTCCCGGCCTGTAG170940.1703425914973596No Hit
CTCCCACTGCTTCACTTGACTAGCCT170670.17007353510503315No Hit
CCCCCCACTGCTAAATTTGACTGG162640.16207159869621252No Hit
TTCACAGTGGCTAAGTTCCGC161290.16072631673458015No Hit
TCGTACGACTCTTAGCGG153810.15327245816198012No Hit
TCGTTTCCCGGCCAATGCACCA153040.15250514919127128No Hit
ACCCCCCACTGCTAAATTTGACTG152490.15195707135505068No Hit
TTCACAGTGGCTAAGTTCTGC149150.14862874413145655No Hit
TTCAAGTAATCCAGGATAGGC144780.14427401659639477No Hit
CCCCCCACTGCTAAATTTGACTGT144450.14394516989466244No Hit
TTATAAAGCAATGAGACTGATT143950.14344691731628006No Hit
TTCACAGTGGCTAAGTTCTG143670.14316789587238593No Hit
TATTGCACTTGTCCCGGCCTGTT143060.1425600277267595No Hit
CTACGCCTGTCTGAGCGTCGCT141990.14149376720902124No Hit
TGATGTAGTAGGTTGTATAGTT140290.13979970844252124No Hit
TTCACAGTGGCTAAGTTCCG137640.13715896977709474No Hit
TACGCCTGTCTGAGCGTCGCT136110.13563431688724473No Hit
TATTGCACTTGTCCCGGCCTGTAAT133760.13329252976884767No Hit
ACCACAGGGTAGAACCACGGAC131620.1311600087333712No Hit
AGCAGCATTGTACAGGGCTATGT128780.1283299340881594No Hit
CCCCCCACTGCTAAATTTGACTGGCT123410.12297870139633292No Hit
CAAAGTGCTGTTCGTGCAGGT120990.12056715891696232No Hit
ATGCACCTGGGCAAGGATTCTGA118690.11827519705640349No Hit
ACTGGACTTGGAGTCAGAAGG116060.1156543884941123No Hit
TGAGAACTGAATTCCATAGGCTGT114130.11373113354155641No Hit
TAGCAGCACGTAAATATTGGCGT112420.11202710972348875No Hit
AGCAGCATTGTACAGGGCTATG112050.11165840281548581No Hit
CAAAGTGCTGTTCGTGCAGGTAG109300.10891801363438285No Hit
CAACGGAATCCCAAAAGCAGCTGA109080.1086987824998946No Hit
TGAGGTAGTAGGTTGTGTGGTT105200.10483234249164754No Hit
TGAGGTAGTAAGTTGTATTGTT103100.10273968166244166No Hit
TATTGCACTTGTCCCGGCCTGTAAA102950.10259020588892695No Hit
CAGTGCAATAGTATTGTCAAAGC101710.1013545394945387No Hit
TAGCTTATCAGACTGATGTTGACC101500.10114527341161812No Hit
ACGCCTGTCTGAGCGTCGCT100520.1001686983579887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)