FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11032398
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10402869.42937337829908No Hit
CCCCCCACTGCTAAATTTGACTG9863448.940431626922814No Hit
AACATTCAACGCTGTCGGTGAGT4732244.2894029022520765No Hit
TAGCAGCACGTAAATATTGGCG4195723.8030897725045816No Hit
AAGCTGCCAGTTGAAGAACTGT4042953.6646157979434753No Hit
TTCAAGTAATCCAGGATAGGCT3704933.3582272865790372No Hit
CCCCCACTGCTAAATTTGACTG3266202.9605530909961733No Hit
AACATTCAACGCTGTCGGTGAG3107842.8170122216403No Hit
TAGCTTATCAGACTGATGTTGACT2531072.2942156365279787No Hit
TAGCTTATCAGACTGATGTTGAC2411662.1859798749102417No Hit
TATTGCACTTGTCCCGGCCTGTAA1949241.7668325598840795No Hit
TATTGCACTTGTCCCGGCCTGTAT1638751.4853978255679319No Hit
TAGCTTATCAGACTGATGTTGA1499591.359260244237019No Hit
TGTAAACATCCTTGACTGGAAGCT1229991.1148890748865297No Hit
TATTGCACTTGTCCCGGCCTGT1005910.9117782008952179No Hit
TGAGGTAGTAGGTTGTATAGTT1005700.9115878524324449No Hit
AGCAGCATTGTACAGGGCTATGA862200.78151640287089No Hit
CATAAAGTAGAAAGCACTACT795480.7210399769841516No Hit
TATTGCACTTGTCCCGGCCTGTA780570.707525236127268No Hit
TGTAAACATCCCCGACTGGAAGCT730930.6625304852127344No Hit
ACTGGACTTGGAGTCAGAAGGC629150.5702749302554169No Hit
TGAGGTAGTAGATTGTATAGTT581000.5266307470053202No Hit
CAACGGAATCCCAAAAGCAGCTGT562470.5098347612187305No Hit
TTCAAGTAATTCAGGATAGGTT541280.49062769490368274No Hit
CACCCGGCTGTGTGCACATGTGC476190.4316287356565635No Hit
TTAATGCTAATCGTGATAGGGGTT447690.4057957299945125No Hit
CCCATAAAGTAGAAAGCACT446530.4047442813430045No Hit
CCCATAAAGTAGAAAGCACTAC437300.39637801319350513No Hit
CGCCTGTCTGAGCGTCGCT425490.3856731782156517No Hit
TGTCAGTTTGTCAAATACCCCAA392600.3558609832603936No Hit
TGTAAACATCCTACACTCTCAGCT386200.3500598872520734No Hit
CATTGCACTTGTCTCGGTCTGA377240.34193835284042506No Hit
CAACGGAATCCCAAAAGCAGCT350310.3175284285429152No Hit
TAGCTTATCAGACTGATGTTGACA350260.31748310748035014No Hit
TTCTCACTACTGCACTTGACTAG339270.3075215379285628No Hit
CTTCTCACTACTGCACTTGACTAG328420.2976868673519574No Hit
AACATTCAACGCTGTCGGTGAGTT312560.28331102630633886No Hit
CAAAGAATTCTCCTTTTGGGCT306960.2782350672990586No Hit
CTCCCACTGCTTCACTTGACTAG304740.27622281212117256No Hit
TGAGGTAGTAGTTTGTACAGTT271160.2457851865025174No Hit
CAACACCAGTCGATGGGCTGT269170.24398140821243033No Hit
CACTAGATTGTGAGCTCCTGGA269120.24393608714986534No Hit
CACTAGATTGTGAGCTCCTGGAA247450.22429393863419356No Hit
CCCCCACTGCTAAATTTGACTGGCTT237430.2152115976961672No Hit
TGTCAGTTTGTCAAATACCCCA235720.2136616173564442No Hit
TATTGCACTTGTCCCGGCCTGTAG233150.2113321147406031No Hit
AACGGAATCCCAAAAGCAGCTG228080.20673655899651192No Hit
TAGCAGCACGTAAATATTGGC226310.20513219338171082No Hit
CTCCCACTGCTTCACTTGACTAGCCT219000.1985062540347076No Hit
CAAAGAATTCTCCTTTTGGGCTT218650.19818900659675256No Hit
TGTAAACATCCCCGACTGGAAGC218570.1981164928966486No Hit
TGAGAACTGAATTCCATAGGCTGT215620.19544255020531348No Hit
ACTGGACTTGGAGTCAGAAGGCA212720.1928139285765434No Hit
TTCAAGTAATCCAGGATAGGCTT206340.18703096099324915No Hit
TGATGTAGTAGGTTGTATAGTT200680.18190061671089094No Hit
TGAGAACTGAATTCCATGGGTT189710.1719571755841296No Hit
ACTGGACTTGGAGTCAGAAGGCT187170.1696548656058275No Hit
TCGTTTCCCGGCCAATGCACCA186330.16889347175473546No Hit
TATTGCACTTGTCCCGGCCTGTT185860.16846745376662445No Hit
TGAGGTAGTAGTTTGTGCTGTT184710.1674250693276294No Hit
TCGTACGACTCTTAGCGG184540.1672709777149084No Hit
CCCCCCACTGCTAAATTTGACTGG179660.16284764200856422No Hit
CTACGCCTGTCTGAGCGTCGCT170770.15478955708450692No Hit
CCCCCCACTGCTAAATTTGACTGGCT165770.15025745082800676No Hit
GCCTGTCTGAGCGTCGCT163480.14818174616252966No Hit
AGCAGCATTGTACAGGGCTATGT158780.1439215662814195No Hit
TATTGCACTTGTCCCGGCCTGTAAT157880.1431057871552495No Hit
TTCAAGTAATCCAGGATAGGC156950.14226281539154045No Hit
ACCACAGGGTAGAACCACGGAC151860.13764913122242325No Hit
TTATAAAGCAATGAGACTGATT146740.1330082544157671No Hit
TACGCCTGTCTGAGCGTCGCT144310.130805650775108No Hit
CCCCCCACTGCTAAATTTGACTGT142900.12952759681077497No Hit
AGCAGCATTGTACAGGGCTATG140490.12734312159514186No Hit
ACTGGACTTGGAGTCAGAAGG134580.12198617199995866No Hit
ATGCACCTGGGCAAGGATTCTGA133130.1206718611855736No Hit
TTCACAGTGGCTAAGTTCTGC131660.11933942194616257No Hit
AACATTCAACGCTGTCGGTGA129610.11748125838099749No Hit
TATTGCACTTGTCCCGGCCTGTAAA128890.11682863508006146No Hit
CAGTGCAATAGTATTGTCAAAGC128410.11639355287943745No Hit
TTCACAGTGGCTAAGTTCTG127360.11544181056557241No Hit
ACTTGACTTGGAGTCAGAAGGC127030.11514269155264341No Hit
TTCACAGTGGCTAAGTTCCG126770.1149070220273054No Hit
ACCCCCCACTGCTAAATTTGACTG122300.11085531903399423No Hit
AGCTCGGTCTGAGGCCCCTCAGT121720.11032959470824023No Hit
TAGCAGCACGTAAATATTGGCGT120610.10932346711929719No Hit
CCCATAAAGTAGAAAGCACTA119700.10849862378061416No Hit
CCCCCCACTGCTAAATTTGACTGTA119650.10845330271804915No Hit
ATCACATTGCCAGGGATTTCC117010.10606035061461706No Hit
TGATGTAGTAGATTGTATAGTT115040.10427470074955598No Hit
GCGTTCGCGCTTTCCCCTG114770.10402996701170497No Hit
CAAAGTGCTGTTCGTGCAGGTAG114040.10336827949825596No Hit
TTCACAGTGGCTAAGTTCCGC113530.10290600466009293No Hit
TATTGCACTTGTCCCGGCCTGTTT113150.10256156458459892No Hit
TGTAAACATCCTACACTCAGCT112590.1020539686838709No Hit
ATCACATTGCCAGGGATTTCCA112580.1020449044713579No Hit
AACATTCAACGCTGTCGGTGAGA112280.10177297809596789No Hit
CTACGGGGATGATTTTACGAACTGAA111980.10150105172057788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)