FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8828771
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7736868.763235562458242No Hit
CCCCCCACTGCTAAATTTGACTG5873066.652182959553487No Hit
AAGCTGCCAGTTGAAGAACTGT3612494.091724657939367No Hit
TTCAAGTAATCCAGGATAGGCT3603744.081813878738048No Hit
AACATTCAACGCTGTCGGTGAGT3303743.742015734692858No Hit
TAGCAGCACGTAAATATTGGCG3239273.668993113537547No Hit
TAGCTTATCAGACTGATGTTGACT2215032.5088769433480604No Hit
AACATTCAACGCTGTCGGTGAG2088222.3652442678601586No Hit
TAGCTTATCAGACTGATGTTGAC1981432.244287455184872No Hit
CCCCCACTGCTAAATTTGACTG1964282.224862327950289No Hit
TATTGCACTTGTCCCGGCCTGTAA1959652.2196181099271914No Hit
TATTGCACTTGTCCCGGCCTGTAT1763841.9978318613088957No Hit
TAGCTTATCAGACTGATGTTGA1312791.4869453517369517No Hit
TGTAAACATCCTTGACTGGAAGCT1122841.271796493532339No Hit
TGAGGTAGTAGGTTGTATAGTT1038211.1759394370971905No Hit
TATTGCACTTGTCCCGGCCTGT979621.109576859565165No Hit
CATAAAGTAGAAAGCACTACT928891.0521169934071233No Hit
TATTGCACTTGTCCCGGCCTGTA788460.8930574821795694No Hit
TTAATGCTAATCGTGATAGGGGTT712140.806612834334473No Hit
TGAGGTAGTAGATTGTATAGTT680610.7709000493953235No Hit
AGCAGCATTGTACAGGGCTATGA668380.7570476117230813No Hit
TTCAAGTAATTCAGGATAGGTT612780.694071689026706No Hit
CCCATAAAGTAGAAAGCACTAC608520.6892465553812643No Hit
TGTAAACATCCCCGACTGGAAGCT596250.6753488112898159No Hit
CAACGGAATCCCAAAAGCAGCTGT488390.5531800519007685No Hit
ACTGGACTTGGAGTCAGAAGGC465200.5269136553660754No Hit
CACCCGGCTGTGTGCACATGTGC444560.5035355430557662No Hit
CCCATAAAGTAGAAAGCACT412030.4666900976364661No Hit
TGTAAACATCCTACACTCTCAGCT396650.4492697794517493No Hit
CAACGGAATCCCAAAAGCAGCT366950.4156297631912754No Hit
CATTGCACTTGTCTCGGTCTGA326380.3696777275115642No Hit
TCGTACGACTCTTAGCGG321820.3645127957220773No Hit
TAGCTTATCAGACTGATGTTGACA304330.3447025639242427No Hit
TGAGGTAGTAGTTTGTACAGTT303400.3436491896777026No Hit
CAAAGAATTCTCCTTTTGGGCT293950.3329455481402791No Hit
TTCTCACTACTGCACTTGACTAG247970.2808658192629529No Hit
TCGTTTCCCGGCCAATGCACCA247700.28056000093331224No Hit
CAACACCAGTCGATGGGCTGT246000.27863447811705616No Hit
CTTCTCACTACTGCACTTGACTAG232920.26381927903668584No Hit
TATTGCACTTGTCCCGGCCTGTAG232100.26289049744296233No Hit
TGTCAGTTTGTCAAATACCCCAA230620.2612141599323394No Hit
AACATTCAACGCTGTCGGTGAGTT228840.25919802427767125No Hit
TGAGAACTGAATTCCATAGGCTGT217100.24590059024070282No Hit
CTCCCACTGCTTCACTTGACTAG207790.2353555211705004No Hit
GCCTGTCTGAGCGTCGCT205770.23306754700059612No Hit
TAGCAGCACGTAAATATTGGC202060.22886537661923725No Hit
CAAAGAATTCTCCTTTTGGGCTT199640.22612433825727274No Hit
CACTAGATTGTGAGCTCCTGGAA199450.22590913276604413No Hit
CGCCTGTCTGAGCGTCGCT198210.22450463377065732No Hit
CACTAGATTGTGAGCTCCTGGA197860.2241082026026046No Hit
TGAGAACTGAATTCCATGGGTT195610.2215597165222657No Hit
TTCAAGTAATCCAGGATAGGCTT195320.22123124498302196No Hit
TATTGCACTTGTCCCGGCCTGTT184880.20940626957024938No Hit
ATCCCGGACGAGCCCCCA179570.2033918424206495No Hit
TGTCAGTTTGTCAAATACCCCA177640.20120580769395877No Hit
TGTAAACATCCCCGACTGGAAGC175360.1986233417992153No Hit
AACGGAATCCCAAAAGCAGCTG172790.19571240436522816No Hit
TGAGGTAGTAGTTTGTGCTGTT170450.1930619788416757No Hit
CCCCCACTGCTAAATTTGACTGGCTT162350.18388742895245555No Hit
ACTGGACTTGGAGTCAGAAGGCA158360.17936811363665453No Hit
TATTGCACTTGTCCCGGCCTGTAAT157590.17849596506693854No Hit
TGATGTAGTAGGTTGTATAGTT153380.17372746444550435No Hit
TTCAAGTAATCCAGGATAGGC149760.16962723350735906No Hit
CACCCGTAGAACCGACCTTGCG148100.167747017110309No Hit
TATTGCACTTGTCCCGGCCTGTAAA146590.16603669978528154No Hit
AGCTCGGTCTGAGGCCCCTCAGT146430.16585547410845747No Hit
AGCAGCATTGTACAGGGCTATGT143890.16297851648887485No Hit
TATTGCACTTGTCCCGGCCTGTTT141580.16036207077972686No Hit
CCCATAAAGTAGAAAGCACTA140850.15953522862921693No Hit
ACTGGACTTGGAGTCAGAAGGCT139270.1577456250705789No Hit
TTCACAGTGGCTAAGTTCTGC139070.15751909297454877No Hit
TTAATGCTAATCGTGATAGGGGTTT131910.14940924393667024No Hit
TTCACAGTGGCTAAGTTCCG131240.1486503614149693No Hit
CTCCCACTGCTTCACTTGACTAGCCT130630.1479594385220774No Hit
TTCACAGTGGCTAAGTTCCGC128520.14556952490895958No Hit
TTATAAAGCAATGAGACTGATT126930.14376859474552006No Hit
TCGTTTCCCGGGCGGCGCACCA119980.13589660440847315No Hit
ATGCACCTGGGCAAGGATTCTGA118660.13440149257467432No Hit
CCCCCCACTGCTAAATTTGACTGGCT117500.1330876064176996No Hit
CCCCCCACTGCTAAATTTGACTGT114480.1296669717676447No Hit
TAGCAGCACGTAAATATTGGCGT112640.1275828764841675No Hit
CCCCCCACTGCTAAATTTGACTGTA110400.1250457170086301No Hit
ATCACATTGCCAGGGATTTCC109470.12399234276208998No Hit
ACCACAGGGTAGAACCACGGAC108750.12317682721638154No Hit
TAGCAGCACATCATGGTTTACA108600.12300692814435894No Hit
TGTAAACATCCTACACTCAGCT108440.12282570246753483No Hit
CAGTGCAATAGTATTGTCAAAGC106890.12107007872330135No Hit
TTCACAGTGGCTAAGTTCTG104070.11787597616927656No Hit
ACTGGACTTGGAGTCAGAAGG103060.11673198908432442No Hit
CAAAGTGCTGTTCGTGCAGGTAG102990.11665270285071389No Hit
CAACGGAATCCCAAAAGCAGCTGA101560.11503299836409847No Hit
CCCCCCACTGCTAAATTTGACTGG100580.11392299109355085No Hit
TGATGTAGTAGATTGTATAGTT100360.11367380578791772No Hit
ATCACATTGCCAGGGATTTCCA96210.10897326479529257No Hit
CAAAGTGCTGTTCGTGCAGGT94900.10748947956629525No Hit
AGCAGCATTGTACAGGGCTATG94170.10666263741578527No Hit
CTACGCCTGTCTGAGCGTCGCT94000.10647008513415966No Hit
TTTAAACATCCTTGACTGGAAGCT92870.10519017879158944No Hit
TGAGGTAGTAGGTTGTGTGGTT92090.10430670361707196No Hit
TATTGCACTTGTCCCGGCCTGA91910.10410282473064483No Hit
AACATTCAACGCTGTCGGTGA90500.10250577345363246No Hit
TGTCAGTTTGTCAAATACCCC90470.10247179363922793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)