FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6900529
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG69945110.136193906293272No Hit
CCCCCCACTGCTAAATTTGACTG5843938.468814492338197No Hit
AACATTCAACGCTGTCGGTGAGT2558533.7077302334357265No Hit
AAGCTGCCAGTTGAAGAACTGT2513013.6417642763330176No Hit
TAGCAGCACGTAAATATTGGCG2473983.5852033952759275No Hit
TTCAAGTAATCCAGGATAGGCT2366403.429302304214648No Hit
AACATTCAACGCTGTCGGTGAG2120813.073402053668639No Hit
CCCCCACTGCTAAATTTGACTG1924872.7894528086180057No Hit
TATTGCACTTGTCCCGGCCTGTAA1183551.7151583595982278No Hit
TAGCTTATCAGACTGATGTTGACT1121821.625701449845367No Hit
TAGCTTATCAGACTGATGTTGAC1077441.5613875399987451No Hit
TATTGCACTTGTCCCGGCCTGTAT956571.3862270559257124No Hit
TGAGGTAGTAGGTTGTATAGTT811331.175750438843167No Hit
TAGCTTATCAGACTGATGTTGA765881.1098859232386387No Hit
TTAATGCTAATCGTGATAGGGGTT669920.9708241208753706No Hit
TATTGCACTTGTCCCGGCCTGT608250.8814541609780931No Hit
TATTGCACTTGTCCCGGCCTGTA596900.8650061466302076No Hit
TGTAAACATCCTTGACTGGAAGCT594030.8608470452047952No Hit
AGCAGCATTGTACAGGGCTATGA438750.6358208189546047No Hit
TGAGGTAGTAGATTGTATAGTT433790.6286329642263658No Hit
CATAAAGTAGAAAGCACTACT424030.614489121051444No Hit
CAACGGAATCCCAAAAGCAGCTGT365590.5297999617130802No Hit
GGAATACCGGGTGCTGTAGGCTTT353280.5119607496758583No Hit
TTCAAGTAATTCAGGATAGGTT342120.49578807653732054No Hit
TGTAAACATCCCCGACTGGAAGCT339360.4917883831804779No Hit
ACTGGACTTGGAGTCAGAAGGC322740.46770327318383853No Hit
CACCCGGCTGTGTGCACATGTGC271580.39356402965627707No Hit
CGCCTGTCTGAGCGTCGCT268890.38966577779761524No Hit
GGAATACCGGGTGCTGTAGGCTT261670.37920281184239646No Hit
CCCATAAAGTAGAAAGCACT246250.3568566989574278No Hit
CAACGGAATCCCAAAAGCAGCT241520.3500021520089257No Hit
CCCATAAAGTAGAAAGCACTAC235560.34136513302096116No Hit
CATTGCACTTGTCTCGGTCTGA215450.31222243975787944No Hit
TGAGGTAGTAGTTTGTACAGTT205420.29768732223283173No Hit
CAAAGAATTCTCCTTTTGGGCT204500.29635409111388417No Hit
AACATTCAACGCTGTCGGTGAGTT192640.27916700299353864No Hit
TGTCAGTTTGTCAAATACCCCAA185660.26905183646065395No Hit
TGTAAACATCCTACACTCTCAGCT183700.2662114745115918No Hit
CAACACCAGTCGATGGGCTGT177890.25779183016258606No Hit
TAGCTTATCAGACTGATGTTGACA172520.25000981808785966No Hit
TTCTCACTACTGCACTTGACTAG168410.24405375298038745No Hit
AACGGAATCCCAAAAGCAGCTG157900.22882303661067144No Hit
CTTCTCACTACTGCACTTGACTAG155990.22605513287459558No Hit
TATTGCACTTGTCCCGGCCTGTAG146570.21240400554798045No Hit
TGAGGTAGTAGTTTGTGCTGTT145760.21123018249760273No Hit
TCGTTTCCCGGCCAATGCACCA145430.21075195829189328No Hit
TAGCAGCACGTAAATATTGGC143180.20749133870751071No Hit
TTCACAGTGGCTAAGTTCCG140960.20427419405091984No Hit
CTCCCACTGCTTCACTTGACTAG133170.1929852044676575No Hit
CACTAGATTGTGAGCTCCTGGAA131420.1904491670131377No Hit
ACTGGACTTGGAGTCAGAAGGCA130100.1885362701902999No Hit
GCCTGTCTGAGCGTCGCT127720.18508725925215297No Hit
CTACGCCTGTCTGAGCGTCGCT127190.18431920219449843No Hit
CACTAGATTGTGAGCTCCTGGA126500.18331927885528776No Hit
TATTGCACTTGTCCCGGCCTGTT122830.17800084602209482No Hit
TTAATGCTAATCGTGATAGGGGTTT119450.17310267082422232No Hit
TCGTACGACTCTTAGCGG119290.17287080454266623No Hit
TGTCAGTTTGTCAAATACCCCA116410.1686972114746565No Hit
TTCACAGTGGCTAAGTTCTG112520.16305996250432395No Hit
CCCCCACTGCTAAATTTGACTGGCTT111860.16210351409290505No Hit
TGAATACCGGGTGCTGTAGGCTTT109600.1588284028659252No Hit
TACGCCTGTCTGAGCGTCGCT107150.1552779504295975No Hit
ACTGGACTTGGAGTCAGAAGGCT106780.15474175965349904No Hit
CAAAGAATTCTCCTTTTGGGCTT100050.14498888418554579No Hit
TTCAAGTAATCCAGGATAGGCTT99800.14462659312061438No Hit
TTCAAGTAATCCAGGATAGGC99030.14351073664062566No Hit
CCCCCCACTGCTAAATTTGACTGG94720.13726483868120837No Hit
TGAGAACTGAATTCCATGGGTT93820.13596059084745532No Hit
ACTGGACTTGGAGTCAGAAGG90810.13159860642568127No Hit
TGAGAACTGAATTCCATAGGCTGT90640.13135224850152794No Hit
CTCCCACTGCTTCACTTGACTAGCCT90540.13120733207555535No Hit
TGTAAACATCCCCGACTGGAAGC89400.1295552848194682No Hit
AGCTCGGTCTGAGGCCCCTCAGT86580.12546864160704202No Hit
TATTGCACTTGTCCCGGCCTGTAAA85430.12380210270835758No Hit
TGATGTAGTAGGTTGTATAGTT84800.12288912922473044No Hit
ACCACAGGGTAGAACCACGGAC84480.12242539666161825No Hit
CCCATAAAGTAGAAAGCACTA83960.12167183124656095No Hit
TATTGCACTTGTCCCGGCCTGTAAT83270.12067190790735029No Hit
ACGCCTGTCTGAGCGTCGCT81990.11881697765490154No Hit
ATGCACCTGGGCAAGGATTCTGA81740.11845468658997015No Hit
CCCCCCACTGCTAAATTTGACTGT81660.11833875344919209No Hit
TGAATACCGGGTGCTGTAGGCTT80890.11722289696920336No Hit
AGCAGCATTGTACAGGGCTATG80620.11683162261907747No Hit
TTAATGCTAATCGTGATAGGGGT79450.11513610043519852No Hit
TGAGGTAGTAGGTTGTGTGGTT78170.11328117018274976No Hit
TTCACAGTGGCTAAGTTCTGC75900.10999156731317265No Hit
ATCACATTGCCAGGGATTTCC75200.10897715233136474No Hit
AGCAGCATTGTACAGGGCTATGT74870.1084989281256553No Hit
GCGTTCGCGCTTTCCCCTG74820.10842646991266901No Hit
TTTAAACATCCTTGACTGGAAGCT73330.10626721516567787No Hit
CCCCCCACTGCTAAATTTGACTGGCT72700.10535424168205075No Hit
CCCCCCACTGCTAAATTTGACTGTA72660.10529627511166172No Hit
ACCCCCCACTGCTAAATTTGACTG71680.10387609413713066No Hit
CAAAGTGCTGTTCGTGCAGGTAG71250.10325295350544864No Hit
TGAGGTAGTAGGTTGTATAGT69570.10081835754910963No Hit
TCGTTTCCCGGGCGGCGCACCA69120.10016623363223312No Hit
AACATTCAACGCTGTCGGTGA69100.10013725034703862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)