FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8182371
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7514359.183585051325588No Hit
CCCCCCACTGCTAAATTTGACTG5782497.067010283449626No Hit
AACATTCAACGCTGTCGGTGAGT3503974.282340656516308No Hit
TAGCAGCACGTAAATATTGGCG3076203.7595459800099507No Hit
TTCAAGTAATCCAGGATAGGCT2899173.5431905984218997No Hit
AAGCTGCCAGTTGAAGAACTGT2697243.2964039396404785No Hit
AACATTCAACGCTGTCGGTGAG2324102.840374752012589No Hit
TAGCTTATCAGACTGATGTTGACT2193292.6805066648774543No Hit
TAGCTTATCAGACTGATGTTGAC1875222.291780707572414No Hit
TATTGCACTTGTCCCGGCCTGTAA1826962.2328002482410048No Hit
CCCCCACTGCTAAATTTGACTG1692502.0684713513968016No Hit
TATTGCACTTGTCCCGGCCTGTAT1589211.9422365473283965No Hit
TAGCTTATCAGACTGATGTTGA1192541.457450413822595No Hit
TGTAAACATCCTTGACTGGAAGCT1114211.3617202153263401No Hit
TGAGGTAGTAGGTTGTATAGTT949251.1601160592693731No Hit
TATTGCACTTGTCCCGGCCTGT698980.854251169984837No Hit
TGTAAACATCCCCGACTGGAAGCT696180.8508291789751407No Hit
CATAAAGTAGAAAGCACTACT696180.8508291789751407No Hit
TATTGCACTTGTCCCGGCCTGTA681810.8332670322575205No Hit
AGCAGCATTGTACAGGGCTATGA642630.7853836009146982No Hit
TGAGGTAGTAGATTGTATAGTT606120.7407632824275506No Hit
CCCATAAAGTAGAAAGCACTAC511660.6253199714361523No Hit
ACTGGACTTGGAGTCAGAAGGC503830.6157506180054656No Hit
TTCAAGTAATTCAGGATAGGTT482100.5891935234909295No Hit
CAACGGAATCCCAAAAGCAGCTGT440560.5384258425827917No Hit
CACCCGGCTGTGTGCACATGTGC388710.47505790192109354No Hit
TGTAAACATCCTACACTCTCAGCT382710.4677250640431728No Hit
CAACGGAATCCCAAAAGCAGCT367360.448965220472159No Hit
TGTCAGTTTGTCAAATACCCCAA350340.42816440369179054No Hit
CAAAGAATTCTCCTTTTGGGCT323270.3950810834659049No Hit
TCGTACGACTCTTAGCGG317560.38810266608541705No Hit
CCCATAAAGTAGAAAGCACT313110.38266414465929255No Hit
TAGCTTATCAGACTGATGTTGACA311470.38065983563932754No Hit
CTTCTCACTACTGCACTTGACTAG279710.34184468047220057No Hit
AACATTCAACGCTGTCGGTGAGTT273900.3347440491270806No Hit
TGAGGTAGTAGTTTGTACAGTT261310.3193573109799103No Hit
TTCTCACTACTGCACTTGACTAG259240.3168274819120277No Hit
CATTGCACTTGTCTCGGTCTGA257090.31419988167243945No Hit
TCGTTTCCCGGCCAATGCACCA241460.295097839000456No Hit
TGTCAGTTTGTCAAATACCCCA229980.28106767586070097No Hit
CTCCCACTGCTTCACTTGACTAG228320.2790389240478096No Hit
CCCCCACTGCTAAATTTGACTGGCTT220090.26898071475859503No Hit
CACTAGATTGTGAGCTCCTGGAA217650.26599869402157394No Hit
TATTGCACTTGTCCCGGCCTGTAG206280.25210296624291423No Hit
TGTAAACATCCCCGACTGGAAGC195980.23951492788581696No Hit
TAGCAGCACGTAAATATTGGC191280.2337708715481124No Hit
CACTAGATTGTGAGCTCCTGGA183790.22461704559717469No Hit
AACGGAATCCCAAAAGCAGCTG183480.22423818230681547No Hit
CTCCCACTGCTTCACTTGACTAGCCT181820.22220943049392405No Hit
ACTGGACTTGGAGTCAGAAGGCA179040.21881188227715417No Hit
CAAAGAATTCTCCTTTTGGGCTT176320.21548766243916342No Hit
CAACACCAGTCGATGGGCTGT173350.21185790768959264No Hit
ACTGGACTTGGAGTCAGAAGGCT167860.2051483610312952No Hit
TATTGCACTTGTCCCGGCCTGTAAA165390.20212967610488453No Hit
TATTGCACTTGTCCCGGCCTGTT156780.19160705375006828No Hit
TGAGGTAGTAGTTTGTGCTGTT156570.19135040442434106No Hit
TATTGCACTTGTCCCGGCCTGTAAT155190.1896638517124193No Hit
CCCCCCACTGCTAAATTTGACTGGCT151100.18466530055897No Hit
TTCAAGTAATCCAGGATAGGCTT147050.17971563499137352No Hit
TTCACAGTGGCTAAGTTCCG135800.16596656397027218No Hit
CCCATAAAGTAGAAAGCACTA135050.1650499592355321No Hit
TTCAAGTAATCCAGGATAGGC134480.16435333963712961No Hit
CCCCCCACTGCTAAATTTGACTGTA131660.1609069058345069No Hit
CGCCTGTCTGAGCGTCGCT131220.16036916439012605No Hit
TATTGCACTTGTCCCGGCCTGTTT128060.15650720310775446No Hit
ACCCCCCACTGCTAAATTTGACTG126160.15418513777974624No Hit
ATGCACCTGGGCAAGGATTCTGA123790.15128866681796757No Hit
ATCACATTGCCAGGGATTTCC122910.15021318392920585No Hit
CCCCCCACTGCTAAATTTGACTGT121930.14901548707581214No Hit
AGCAGCATTGTACAGGGCTATGT120320.14704784224523676No Hit
AGCTCGGTCTGAGGCCCCTCAGT117250.14329587353103398No Hit
TAGCAGCACATCATGGTTTACA114430.13984943972841124No Hit
ATCACATTGCCAGGGATTTCCA114070.139409469455736No Hit
AACATTCAACGCTGTCGGTGA110080.1345331322669187No Hit
ACTGGACTTGGAGTCAGAAGG108980.1331887786559666No Hit
CTACGGGGATGATTTTACGAACTGAA106280.12988900161090228No Hit
TTCACAGTGGCTAAGTTCTGC104410.12760360047228364No Hit
CCCCCCACTGCTAAATTTGACTGG103530.12652811758352195No Hit
TTATAAAGCAATGAGACTGATT103410.12638146082596352No Hit
CAGTGCAATAGTATTGTCAAAGC101470.1240105099121025No Hit
TTCACAGTGGCTAAGTTCTG100880.12328944752077363No Hit
ATCCCGGACGAGCCCCCA96370.11777759771586989No Hit
TAGCAGCACGTAAATATTGGCGT95790.11706875672100421No Hit
TGTAAACATCCTACACTCAGCT95360.11654323667308657No Hit
AACATTCAACGCTGTCGGTGAGA95030.11613993058980092No Hit
TTAATGCTAATCGTGATAGGGGTT93860.11471002720360639No Hit
TTCACAGTGGCTAAGTTCCGC88980.1087459857295642No Hit
CAAAGTGCTGTTCGTGCAGGTAG87700.10718164698227445No Hit
AGCAGCATTGTACAGGGCTATG85250.10418740484879016No Hit
CCTTGGCTTGGCTCTTGACGTGGACA84670.1034785638539245No Hit
TGTCAGTTTGTCAAATACCCC84520.10329524290697648No Hit
TGAGGTAGTAGGTTGTGTGGTT82850.10125426969762187No Hit
ACCACAGGGTAGAACCACGGAC82830.10122982690469547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)