FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10454487
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7465707.141144276137126No Hit
AAGCTGCCAGTTGAAGAACTGT5492595.253811114787363No Hit
CCCCCCACTGCTAAATTTGACTG4875814.663844337842689No Hit
TTCAAGTAATCCAGGATAGGCT4836844.626568477248095No Hit
TAGCAGCACGTAAATATTGGCG4413554.221680126437576No Hit
AACATTCAACGCTGTCGGTGAGT3942273.7708880407044365No Hit
TAGCTTATCAGACTGATGTTGAC3386833.2395946352987No Hit
TAGCTTATCAGACTGATGTTGACT2750682.6310999286717753No Hit
TAGCTTATCAGACTGATGTTGA2492812.384440288653092No Hit
AACATTCAACGCTGTCGGTGAG2145492.052219300669655No Hit
CCCCCACTGCTAAATTTGACTG1672921.6001932949938145No Hit
TGTAAACATCCTTGACTGGAAGCT1658101.5860175635590728No Hit
TATTGCACTTGTCCCGGCCTGTAA1398451.3376553053248812No Hit
CATAAAGTAGAAAGCACTACT1343861.2854384916256532No Hit
TATTGCACTTGTCCCGGCCTGTAT1319471.2621087959648332No Hit
TGAGGTAGTAGGTTGTATAGTT1152451.1023496418332148No Hit
TATTGCACTTGTCCCGGCCTGT938140.8973563217401294No Hit
TGAGGTAGTAGATTGTATAGTT934460.8938363020586281No Hit
TTCAAGTAATTCAGGATAGGTT863280.8257507039800231No Hit
AGCAGCATTGTACAGGGCTATGA834620.7983366376561567No Hit
TGTAAACATCCCCGACTGGAAGCT678870.6493575437991361No Hit
TGTAAACATCCTACACTCTCAGCT638200.6104555871560221No Hit
TGTCAGTTTGTCAAATACCCCAA619910.5929607067281254No Hit
GCCTGTCTGAGCGTCGCT603970.5777136649555353No Hit
TATTGCACTTGTCCCGGCCTGTA599440.5733805972497742No Hit
TTCTCACTACTGCACTTGACTAG561750.5373290913270063No Hit
CCCATAAAGTAGAAAGCACTAC547970.5241481480631236No Hit
ACTGGACTTGGAGTCAGAAGGC502960.4810948638608475No Hit
CAACGGAATCCCAAAAGCAGCTGT490110.4688034907882137No Hit
TGAGGTAGTAGTTTGTACAGTT444360.42504237654128796No Hit
TGTCAGTTTGTCAAATACCCCA414760.39672917475529884No Hit
CTTCTCACTACTGCACTTGACTAG410650.3927978484262308No Hit
CCCATAAAGTAGAAAGCACT405280.38766129796708343No Hit
TAGCTTATCAGACTGATGTTGACA393900.37677601971287544No Hit
CATTGCACTTGTCTCGGTCTGA384660.3679377094256275No Hit
CGCCTGTCTGAGCGTCGCT376200.3598454902665238No Hit
CTCCCACTGCTTCACTTGACTAG360640.3449619287871323No Hit
TGTAAACATCCTACACTCAGCT331300.3168974240438579No Hit
CAAAGAATTCTCCTTTTGGGCT312050.29848427761209134No Hit
AACATTCAACGCTGTCGGTGAGTT301230.2881346545268075No Hit
CAACACCAGTCGATGGGCTGT296000.28313201785989117No Hit
TGATGTAGTAGGTTGTATAGTT267930.2562823025175697No Hit
TTCAAGTAATCCAGGATAGGCTT266600.255010121491375No Hit
TGAGGTAGTAGTTTGTGCTGTT255790.24467006367696476No Hit
CCCCCACTGCTAAATTTGACTGGCTT252260.24129352305856805No Hit
CAAAGAATTCTCCTTTTGGGCTT249070.23824220164987533No Hit
TGTAAACATCCCCGACTGGAAGC240130.22969084948883672No Hit
TAGCAGCACGTAAATATTGGC231130.2210821057025562No Hit
TTCAAGTAATCCAGGATAGGC229840.21984818575985604No Hit
TTATAAAGCAATGAGACTGATT226790.21693077814339431No Hit
CCCCCCACTGCTAAATTTGACTGGCT225450.2156490318463259No Hit
TTCACAGTGGCTAAGTTCCG223720.21399423998518532No Hit
TTCACAGTGGCTAAGTTCCGC220420.21083770059688248No Hit
TGATGTAGTAGATTGTATAGTT214330.2050124506348327No Hit
AACCGGGCGGAAACACCA208770.19969416002908608No Hit
TAGCAGCACGTAAATATTGGCGT205860.1969106662048554No Hit
TGTCAGTTTGTCAAATACCCC201120.1923767278107477No Hit
AGCTCGGTCTGAGGCCCCTCAGT199600.19092280663795363No Hit
TTCACAGTGGCTAAGTTCTGC192440.18407407269242385No Hit
CTACGCCTGTCTGAGCGTCGCT192240.18388276727495095No Hit
CTCCCACTGCTTCACTTGACTAGCCT183200.17523576240517588No Hit
CACCCGGCTGTGTGCACATGTGC180940.1730740111877321No Hit
TATTGCACTTGTCCCGGCCTGTT168280.16096437826169757No Hit
TCGTACGACTCTTAGCGG168040.1607348117607301No Hit
AGCAGCATTGTACAGGGCTATGT165090.15791305685300483No Hit
CAACGGAATCCCAAAAGCAGCT160120.1531591172288033No Hit
CCCCCCACTGCTAAATTTGACTGG159480.15254693989289No Hit
AACATTCAACGCTGTCGGTGA158470.15158084753465187No Hit
AACGGAATCCCAAAAGCAGCTG157310.15047127611330904No Hit
TAGCTTATCAGACTGATGTTG155670.14890257169003127No Hit
TACGCCTGTCTGAGCGTCGCT154540.14782169608130938No Hit
TATTGCACTTGTCCCGGCCTGTAG149920.14340254093768542No Hit
TGTAAACATCCTTGACTGGAAGC146770.14038948061248724No Hit
ATCACATTGCCAGGGATTTCCA145430.13910773431541884No Hit
ATCACATTGCCAGGGATTTCC142530.13633380576206178No Hit
GGAATACCGGGTGCTGTAGGCTTT140850.13472684025528942No Hit
TAGCAGCACATCATGGTTTACA138980.1329381346019178No Hit
ACTGGACTTGGAGTCAGAAGGCA138580.13255552376697202No Hit
CAAAGTGCTGTTCGTGCAGGT136630.13069029594661125No Hit
CACTAGATTGTGAGCTCCTGGA130920.12522852627775996No Hit
TAGCAGCACATAATGGTTTGT127770.1222154659525618No Hit
AGCAGCATTGTACAGGGCTATG124220.11881979479241783No Hit
CACTAGATTGTGAGCTCCTGGAA122990.1176432664749595No Hit
CAAAGTGCTGTTCGTGCAGGTAG122090.11678239209633147No Hit
AACATTCAACGCTGTCGGTGAGA122070.11676326155458418No Hit
ACCACAGGGTAGAACCACGGAC122050.11674413101283687No Hit
ACTTGACTTGGAGTCAGAAGGC116450.1113875793235957No Hit
GTAAACATCCTTGACTGGAAGCT116020.11097627267602896No Hit
TGAGGTAGTAGGTTGTGTGGTT115170.11016322465176914No Hit
ACTGGACTTGGAGTCAGAAGG114230.1092640891896465No Hit
CCCATAAAGTAGAAAGCACTA113710.10876669510421696No Hit
ACGCCTGTCTGAGCGTCGCT111990.10712146851395005No Hit
ATCCCACCGCTGCCACCA110730.10591624438387078No Hit
GCGTTCGCGCTTTCCCCTG110090.1053040670479575No Hit
TATTGCACTTGTCCCGGCCTGTTT109890.1051127616304846No Hit
CAGTGCAATAGTATTGTCAAAGC109440.10468232444117057No Hit
TTCACAGTGGCTAAGTTCTG109210.10446232321107674No Hit
ACTGGACTTGGAGTCAGAAGGCT108220.10351536139458588No Hit
AGAGGTAGTAGGTTGCATAGTT106860.10221448455577017No Hit
TGAGGTAGTAGGTTGTATAGT106690.10205187495091822No Hit
TATTGCACTTGTCCCGGCCTGTAAT106570.10193709170043445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)