FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10169425
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7575297.449083896090487No Hit
AAGCTGCCAGTTGAAGAACTGT4851894.771056377327135No Hit
CCCCCCACTGCTAAATTTGACTG4422914.34922328450232No Hit
TTCAAGTAATCCAGGATAGGCT4065033.997305649041121No Hit
AACATTCAACGCTGTCGGTGAGT4056563.9889767612229794No Hit
TAGCAGCACGTAAATATTGGCG3852323.7881394474122185No Hit
TAGCTTATCAGACTGATGTTGAC3067343.0162373978863113No Hit
TAGCTTATCAGACTGATGTTGACT2472432.4312387376867424No Hit
TAGCTTATCAGACTGATGTTGA2128782.093314027095927No Hit
AACATTCAACGCTGTCGGTGAG2111502.0763219159392No Hit
CCCCCACTGCTAAATTTGACTG1576821.5505498098466728No Hit
TGTAAACATCCTTGACTGGAAGCT1533041.5074991948905665No Hit
TATTGCACTTGTCCCGGCCTGTAA1522711.4973412951076388No Hit
TATTGCACTTGTCCCGGCCTGTAT1351801.3292786956981344No Hit
CATAAAGTAGAAAGCACTACT1036261.0189956659299813No Hit
TGAGGTAGTAGGTTGTATAGTT1003150.9864372862772477No Hit
TATTGCACTTGTCCCGGCCTGT883750.8690265182151401No Hit
AGCAGCATTGTACAGGGCTATGA842010.827981916381703No Hit
TGAGGTAGTAGATTGTATAGTT784630.7715578806078023No Hit
TTCAAGTAATTCAGGATAGGTT729770.7176118610442577No Hit
TGTAAACATCCCCGACTGGAAGCT717430.7054774483316412No Hit
CCCATAAAGTAGAAAGCACTAC622630.6122568385134853No Hit
TGTAAACATCCTACACTCTCAGCT612610.602403774058022No Hit
TATTGCACTTGTCCCGGCCTGTA608430.5982934138360821No Hit
TTAATGCTAATCGTGATAGGGGTT575150.5655678664231262No Hit
TGTCAGTTTGTCAAATACCCCAA553090.5438753911848506No Hit
CAACGGAATCCCAAAAGCAGCTGT499230.49091271138732034No Hit
TAGCTTATCAGACTGATGTTGACA428270.4211349215909454No Hit
ACTGGACTTGGAGTCAGAAGGC417610.4106525196852329No Hit
TTCTCACTACTGCACTTGACTAG414350.40744683204802634No Hit
TGTCAGTTTGTCAAATACCCCA378250.37194826649491No Hit
CAAAGAATTCTCCTTTTGGGCT358460.35248797252548697No Hit
CCCATAAAGTAGAAAGCACT347180.3413958999648456No Hit
TGAGGTAGTAGTTTGTACAGTT340280.3346108555793469No Hit
AACATTCAACGCTGTCGGTGAGTT325410.31998859325871426No Hit
CATTGCACTTGTCTCGGTCTGA323940.3185430838026732No Hit
TGATGTAGTAGGTTGTATAGTT312760.3075493452186333No Hit
CGCCTGTCTGAGCGTCGCT299960.29496259621365023No Hit
CTTCTCACTACTGCACTTGACTAG295800.29087190278703073No Hit
CCCCCACTGCTAAATTTGACTGGCTT292330.2874597137989611No Hit
TGTAAACATCCTACACTCAGCT285390.28063533582282185No Hit
AACCGGGCGGAAACACCA270260.26575740516302543No Hit
CAAAGAATTCTCCTTTTGGGCTT267660.26320072177138826No Hit
GCCTGTCTGAGCGTCGCT259790.2554618378128557No Hit
TGATGTAGTAGATTGTATAGTT244200.24013157086069273No Hit
TGTAAACATCCCCGACTGGAAGC241220.23720121835797012No Hit
TTCAAGTAATCCAGGATAGGCTT238950.2349690370891176No Hit
TAGCAGCACGTAAATATTGGC226880.22310012611332497No Hit
TGAGGTAGTAGTTTGTGCTGTT213380.2098250392721319No Hit
CTCCCACTGCTTCACTTGACTAG210580.20707168792729186No Hit
CACCCGGCTGTGTGCACATGTGC201700.19833963080508485No Hit
CCCCCCACTGCTAAATTTGACTGGCT197360.19407193622058277No Hit
CAACACCAGTCGATGGGCTGT194920.19167258719150787No Hit
TGAGAACTGAATTCCATGGGTT190500.18732622542572466No Hit
TAGCAGCACGTAAATATTGGCGT188530.18538904608667647No Hit
CTACGCCTGTCTGAGCGTCGCT187250.18413037118617817No Hit
AGCTCGGTCTGAGGCCCCTCAGT181390.17836800015733437No Hit
TTATAAAGCAATGAGACTGATT173690.17079628395902424No Hit
ATCACATTGCCAGGGATTTCCA171760.16889843821061662No Hit
CAACGGAATCCCAAAAGCAGCT170880.16803309921652404No Hit
TTCACAGTGGCTAAGTTCCGC170390.16755126273117704No Hit
TGTCAGTTTGTCAAATACCCC169280.16645975559090115No Hit
TTCACAGTGGCTAAGTTCCG166440.16366707065542052No Hit
TTCAAGTAATCCAGGATAGGC164190.16145455618188836No Hit
TATTGCACTTGTCCCGGCCTGTAG163480.1607563849480182No Hit
TTCACAGTGGCTAAGTTCTGC161850.1591535411294149No Hit
TATTGCACTTGTCCCGGCCTGTT161120.15843570310022445No Hit
ATCACATTGCCAGGGATTTCC158980.15633135600095383No Hit
TCGTACGACTCTTAGCGG155620.15302733438714577No Hit
AGCAGCATTGTACAGGGCTATGT151500.14897597455116685No Hit
CCCCCCACTGCTAAATTTGACTGG151270.14874980640498356No Hit
TGAGAACTGAATTCCATAGGCTGT148350.1458784542882218No Hit
TAGCAGCACATCATGGTTTACA144020.14162059310137987No Hit
ACTTGACTTGGAGTCAGAAGGC141540.1391819104816644No Hit
CTCCCACTGCTTCACTTGACTAGCCT139160.13684156183855037No Hit
AACGGAATCCCAAAAGCAGCTG137120.1348355487158812No Hit
GGAATACCGGGTGCTGTAGGCTTT136980.13469788114863918No Hit
ACTGGACTTGGAGTCAGAAGGCA136650.13437337902585444No Hit
TCGTTTCCCGGCCAATGCACCA134950.13270170142363014No Hit
AACATTCAACGCTGTCGGTGA132190.12998768366943067No Hit
TGTAAACATCCTTGACTGGAAGC130690.12851267402040922No Hit
AACATTCAACGCTGTCGGTGAGA130550.1283750064531672No Hit
TACGCCTGTCTGAGCGTCGCT130090.12792267016080064No Hit
TATTGCACTTGTCCCGGCCTGTAAT124520.12244546766410097No Hit
CACTAGATTGTGAGCTCCTGGAA123690.1216292956583091No Hit
CAAAGTGCTGTTCGTGCAGGTAG122200.1201641194069478No Hit
TAGCTTATCAGACTGATGTTG114590.11268090378757895No Hit
TATTGCACTTGTCCCGGCCTGTTT113540.11164839703326393No Hit
CAGTGCAATAGTATTGTCAAAGC112980.11109772676429593No Hit
CACTAGATTGTGAGCTCCTGGA112240.11037005533744533No Hit
CAAAGTGCTGTTCGTGCAGGT111520.10966205070591503No Hit
TATTGCACTTGTCCCGGCCTGTAAA109740.10791170592240958No Hit
ACCACAGGGTAGAACCACGGAC109230.1074102026417423No Hit
CCCATAAAGTAGAAAGCACTA106470.1046961848875428No Hit
AGCAGCATTGTACAGGGCTATG106390.10461751770626167No Hit
ACGCCTGTCTGAGCGTCGCT104690.10294584010403734No Hit
TGATGTAGTAGTTTGTACAGTT104170.10243450342570992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)