FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10599726
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8737798.243411197610202No Hit
CCCCCCACTGCTAAATTTGACTG5366135.06251765375822No Hit
AACATTCAACGCTGTCGGTGAGT4781554.511012831841125No Hit
AAGCTGCCAGTTGAAGAACTGT3853313.635292082078348No Hit
TTCAAGTAATCCAGGATAGGCT3697683.4884675320852634No Hit
TAGCAGCACGTAAATATTGGCG3314603.127061963677175No Hit
AACATTCAACGCTGTCGGTGAG3082152.9077638421974306No Hit
TAGCTTATCAGACTGATGTTGAC2216512.091101222805193No Hit
CCCCCACTGCTAAATTTGACTG2120892.0008913437951135No Hit
TAGCTTATCAGACTGATGTTGACT1914921.8065750001462302No Hit
TAGCTTATCAGACTGATGTTGA1814901.7122140704391793No Hit
TATTGCACTTGTCCCGGCCTGTAA1530121.4435467482838706No Hit
TATTGCACTTGTCCCGGCCTGTAT1336011.2604193731045499No Hit
TGAGGTAGTAGGTTGTATAGTT1297061.2236731402302286No Hit
TATTGCACTTGTCCCGGCCTGT1141421.0768391560310144No Hit
TGTAAACATCCTTGACTGGAAGCT1080871.019715037917018No Hit
CATAAAGTAGAAAGCACTACT1055370.9956578122868459No Hit
AGCAGCATTGTACAGGGCTATGA838280.7908506314219821No Hit
GCCTGTCTGAGCGTCGCT836720.7893788952657833No Hit
TGAGGTAGTAGATTGTATAGTT833190.7860486205020771No Hit
TATTGCACTTGTCCCGGCCTGTA820530.7741049155421564No Hit
TTAATGCTAATCGTGATAGGGGTT700570.660932178812924No Hit
ACTGGACTTGGAGTCAGAAGGC666430.628723799086882No Hit
CCCATAAAGTAGAAAGCACTAC647350.6107233337918357No Hit
TGTAAACATCCCCGACTGGAAGCT629580.5939587494997512No Hit
CGCCTGTCTGAGCGTCGCT619960.5848830432031923No Hit
TTCAAGTAATTCAGGATAGGTT542780.5120698402958719No Hit
CCCATAAAGTAGAAAGCACT537760.5073338688188733No Hit
CAACGGAATCCCAAAAGCAGCTGT496410.46832342647347674No Hit
TCGTACGACTCTTAGCGG467160.44072837354475014No Hit
TGTAAACATCCTACACTCTCAGCT399940.377311639942391No Hit
TGTCAGTTTGTCAAATACCCCAA395290.37292473409218313No Hit
AACATTCAACGCTGTCGGTGAGTT394290.37198131347923524No Hit
TGAGGTAGTAGTTTGTACAGTT392680.3704624062923891No Hit
CATTGCACTTGTCTCGGTCTGA377400.35604693932654485No Hit
CAACACCAGTCGATGGGCTGT371300.35029207358756254No Hit
AACCGGGCGGAAACACCA332860.3140269852258445No Hit
TAGCTTATCAGACTGATGTTGACA311770.2941302444987729No Hit
TGAGGTAGTAGTTTGTGCTGTT296230.2794694881735622No Hit
TTCTCACTACTGCACTTGACTAG295460.2787430543015923No Hit
CCCCCACTGCTAAATTTGACTGGCTT292870.2762995949140572No Hit
CAAAGAATTCTCCTTTTGGGCT252720.23842125730419825No Hit
TCCTGTCTGAGCGTCGCT247810.23378906209462397No Hit
TTCACAGTGGCTAAGTTCCG244780.2309304976373918No Hit
AACGGAATCCCAAAAGCAGCTG243800.2300059454367028No Hit
CTACGCCTGTCTGAGCGTCGCT241350.2276945649349804No Hit
CACCCGGCTGTGTGCACATGTGC239440.22589263156424988No Hit
TACGCCTGTCTGAGCGTCGCT238290.22480769785935975No Hit
CAACGGAATCCCAAAAGCAGCT235110.22180762031018536No Hit
TGTCAGTTTGTCAAATACCCCA234330.22107175223208597No Hit
CTCCCACTGCTTCACTTGACTAG228110.21520367601954993No Hit
TGAGAACTGAATTCCATGGGTT221000.20849595546149022No Hit
TATTGCACTTGTCCCGGCCTGTT217820.20549587791231583No Hit
CTTCTCACTACTGCACTTGACTAG215710.20350526041899575No Hit
ACTGGACTTGGAGTCAGAAGGCA214240.2021184321179623No Hit
CCCCCCACTGCTAAATTTGACTGGCT194730.18371229595934838No Hit
TGAGGTAGTAGGTTGTGTGGTT189880.179136705986551No Hit
ACTGGACTTGGAGTCAGAAGGCT189560.17883481139040763No Hit
TAGCAGCACGTAAATATTGGC186690.17612719423124712No Hit
TTCAAGTAATCCAGGATAGGCTT182080.1717780252055572No Hit
CACTAGATTGTGAGCTCCTGGA181660.17138178854811909No Hit
ATCCCGGACGAGCCCCCA180150.16995722342256772No Hit
TATTGCACTTGTCCCGGCCTGTAG177390.16735338253083146No Hit
TGTAAACATCCCCGACTGGAAGC174650.16476841005135417No Hit
TTCAAGTAATCCAGGATAGGC171140.16145700369990695No Hit
AGCTCGGTCTGAGGCCCCTCAGT170350.16071170141567812No Hit
ACTGGACTTGGAGTCAGAAGG168670.15912675478592558No Hit
TGTAAACATCCTACACTCAGCT168440.15890976804494755No Hit
TCGTTTCCCGGCCAATGCACCA167610.1581267289362008No Hit
AACATTCAACGCTGTCGGTGA165150.1558059142283489No Hit
CACTAGATTGTGAGCTCCTGGAA165000.15566440113640673No Hit
TTATAAAGCAATGAGACTGATT163970.15469267790507038No Hit
AGCAGCATTGTACAGGGCTATGT159290.15027746943647413No Hit
GGAATACCGGGTGCTGTAGGCTTT156180.14734343133020608No Hit
CCCATAAAGTAGAAAGCACTA152950.1442961827503843No Hit
CAAAGAATTCTCCTTTTGGGCTT150570.14205084169156826No Hit
CTCCCACTGCTTCACTTGACTAGCCT148050.13967342174693947No Hit
TGAGAACTGAATTCCATAGGCTGT144750.13656013372421139No Hit
AACATTCAACGCTGTCGGTGAGA142920.13483367400251667No Hit
CCCCCCACTGCTAAATTTGACTGG142530.13446573996346697No Hit
ACGCCTGTCTGAGCGTCGCT140700.13273928024177228No Hit
AGCAGCATTGTACAGGGCTATG139380.13149396503268104No Hit
ACCACAGGGTAGAACCACGGAC137580.12979580792937478No Hit
TTCACAGTGGCTAAGTTCTG132420.12492775756656352No Hit
ATCACATTGCCAGGGATTTCC130530.12314469260809194No Hit
TAGCAGCACGTAAATATTGGCGT130310.1229371400732434No Hit
AGCTACATTGTCTGCTGGGTTT127610.12038990441828401No Hit
TTCACAGTGGCTAAGTTCTGC125050.11797474764913733No Hit
TGAGGTAGTAGGTTGTATAGT123600.11660678776036287No Hit
ATCCCACCGCTGCCACCA116480.10988963299617367No Hit
TTCACAGTGGCTAAGTTCCGC113630.1072008842492721No Hit
CAAAGTGCTGTTCGTGCAGGTAG113430.10701220012668251No Hit
CAAAGTGCTGTTCGTGCAGGT111240.10494610898432656No Hit
GGAATACCGGGTGCTGTAGGCTT111110.10482346430464334No Hit
AGAGGTAGTAGGTTGCATAGTT111040.10475742486173699No Hit
TCGTTTCCCGGGCGGCGCACCA106920.10087053193639156No Hit
CCCCCCACTGCTAAATTTGACTGTA106470.10044599266056499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)