FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10689780
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8055237.535449747328757No Hit
CAACGGAATCCCAAAAGCAGCTG7208146.743019968605528No Hit
GCCTGTCTGAGCGTCGCT5362685.016642063728159No Hit
AACATTCAACGCTGTCGGTGAGT4192733.9221854893178345No Hit
AAGCTGCCAGTTGAAGAACTGT4112953.8475534576015593No Hit
TTCAAGTAATCCAGGATAGGCT3519353.292256716228023No Hit
CCCCCACTGCTAAATTTGACTG2954712.764051271401282No Hit
TAGCAGCACGTAAATATTGGCG2942892.7529939811670583No Hit
AACATTCAACGCTGTCGGTGAG2072641.9388986489899698No Hit
TCCTGTCTGAGCGTCGCT1520721.4225924200498046No Hit
TGAGGTAGTAGGTTGTATAGTT1397061.3069118354166316No Hit
TAGCTTATCAGACTGATGTTGAC1372321.2837682347064205No Hit
CGCCTGTCTGAGCGTCGCT1368181.2798953767056012No Hit
TAGCTTATCAGACTGATGTTGACT1219171.1405005528645116No Hit
TATTGCACTTGTCCCGGCCTGT1092811.0222941912742824No Hit
TATTGCACTTGTCCCGGCCTGTAA1083571.013650421243468No Hit
TAGCTTATCAGACTGATGTTGA1063870.9952216041864286No Hit
CATAAAGTAGAAAGCACTACT1037680.9707215677029837No Hit
TATTGCACTTGTCCCGGCCTGTAT1015090.9495892338289468No Hit
TGTAAACATCCTTGACTGGAAGCT782750.7322414493095274No Hit
TGAGGTAGTAGATTGTATAGTT759840.7108097640924322No Hit
AGCAGCATTGTACAGGGCTATGA703420.6580303804194286No Hit
TCGTACGACTCTTAGCGG684810.6406212288746822No Hit
TATTGCACTTGTCCCGGCCTGTA604530.5655214606848784No Hit
ACTGGACTTGGAGTCAGAAGGC600850.5620789202397056No Hit
CCCATAAAGTAGAAAGCACTAC588880.5508813090634233No Hit
AACCGGGCGGAAACACCA519830.4862869020690791No Hit
CCCATAAAGTAGAAAGCACT496380.46435006146057267No Hit
TTCAAGTAATTCAGGATAGGTT480800.44977539294541147No Hit
TGTAAACATCCCCGACTGGAAGCT459330.42969078877207956No Hit
CAACGGAATCCCAAAAGCAGCTGT458310.42873660636608046No Hit
GGAATACCGGGTGCTGTAGGCTTT397640.37198146266808113No Hit
CATTGCACTTGTCTCGGTCTGA374670.3504936490741624No Hit
TACGCCTGTCTGAGCGTCGCT373750.3496330139628692No Hit
CAACACCAGTCGATGGGCTGT360530.3372660616027645No Hit
TTCTCACTACTGCACTTGACTAG358170.335058345447708No Hit
TGAGGTAGTAGTTTGTACAGTT343800.3216155991984868No Hit
TGTAAACATCCTACACTCTCAGCT318470.2979200694495116No Hit
AACATTCAACGCTGTCGGTGAGTT306690.2869001981331702No Hit
CTACGCCTGTCTGAGCGTCGCT302970.283420238770115No Hit
ACGCCTGTCTGAGCGTCGCT296010.27690934705859244No Hit
GGAATACCGGGTGCTGTAGGCTT290260.2715303776130098No Hit
CTCCCACTGCTTCACTTGACTAG285370.26695591490189696No Hit
TGTCAGTTTGTCAAATACCCCAA280580.2624749994854899No Hit
CTTCTCACTACTGCACTTGACTAG253780.23740432450433968No Hit
ATCCCACCGCTGCCACCA222710.20833918003925245No Hit
ATCCCGGACGAGCCCCCA222330.20798370031937047No Hit
TGAGGTAGTAGTTTGTGCTGTT213100.19934928501802654No Hit
AACGGAATCCCAAAAGCAGCTG211800.19813317018685137No Hit
GCGTTCGCGCTTTCCCCTG204190.19101422105974117No Hit
CAACGGAATCCCAAAAGCAGCT202520.18945198123815457No Hit
CCCCCCACTGCTAAATTTGACTGG199870.1869729779284513No Hit
CCCCCACTGCTAAATTTGACTGGCTT199640.18675781915062797No Hit
TGAGGTAGTAGGTTGTGTGGTT195990.18334334289386683No Hit
CCCCCCACTGCTAAATTTGACTGGCT188230.17608407282469798No Hit
TTCAAGTAATCCAGGATAGGCTT178940.1673935291465306No Hit
TTCACAGTGGCTAAGTTCCG172260.16114456986018422No Hit
ACCACAGGGTAGAACCACGGAC171200.1601529685363029No Hit
ACCGGGTGCTGTAGGCTT170990.15995651921742077No Hit
TATTGCACTTGTCCCGGCCTGTT169320.15839427939583414No Hit
TCCTTCTGATCGAGGCCC165680.15498915786854361No Hit
AGCTCGGTCTGAGGCCCCTCAGT160720.1503492120511367No Hit
TTCAAGTAATCCAGGATAGGC158870.14861858709907969No Hit
TGTCAGTTTGTCAAATACCCCA156080.1460086175767883No Hit
CCCTGTCTGAGCGTCGCT154160.14421250951843723No Hit
AACATTCAACGCTGTCGGTGA151800.14200479336338073No Hit
TAGCAGCACGTAAATATTGGC149010.13939482384108934No Hit
AGCAGCATTGTACAGGGCTATGT146820.13734613808703267No Hit
CAAAGTGCTGTTCGTGCAGGT146130.13670066175356274No Hit
TGTAAACATCCCCGACTGGAAGC145690.1362890536568573No Hit
TTATAAAGCAATGAGACTGATT145320.1359429286664459No Hit
CACTAGATTGTGAGCTCCTGGA144120.13482036112997647No Hit
ACTGGACTTGGAGTCAGAAGG143930.13464262127003548No Hit
ACTGGACTTGGAGTCAGAAGGCA142390.13320199293156643No Hit
AGCAGCATTGTACAGGGCTATG140140.13109717880068628No Hit
CAAAGAATTCTCCTTTTGGGCT139840.13081653691656892No Hit
TGAGGTAGTAGGTTGTATAGT138500.1295630031675114No Hit
TAGCTTATCAGACTGATGTTGACA136790.12796334442804247No Hit
ACTGGACTTGGAGTCAGAAGGCT135520.12677529378527902No Hit
TGTAAACATCCTACACTCAGCT134200.12554046949516268No Hit
ACCGGGTGCTGTAGGCTTT132930.12435241885239921No Hit
GACCCGCGGGCGCTCTCCAGTCCT130710.1222756689099308No Hit
AGAGGTAGTAGGTTGCATAGTT130300.12189212500163708No Hit
TTCACAGTGGCTAAGTTCCGC129950.12156470947016684No Hit
TATTGCACTTGTCCCGGCCTGTAG128310.12003053383699196No Hit
TTCACAGTGGCTAAGTTCTGC126800.11861796968693462No Hit
TGAATACCGGGTGCTGTAGGCTTT126230.11808475010711166No Hit
TAGCAGCACGTAAATATTGGCGT125200.11712121297164207No Hit
CAGGTCCCTGTTCGGGCGCCA123830.11583961503417284No Hit
CTCCCACTGCTTCACTTGACTAGCCT121010.11320158132346972No Hit
CAAAGAATTCTCCTTTTGGGCTT118120.11049806450647254No Hit
ACCGGGTGCTGTAGGCTTA117880.11027355099917865No Hit
AATGACACGATCACTCCCGTTG115290.10785067606629885No Hit
CCCATAAAGTAGAAAGCACTA112900.1056148957228306No Hit
CCCCCCACTGCTAAATTTGACTGTA112820.10554005788706597No Hit
AACATTCAACGCTGTCGGTGAGA110540.10340717956777408No Hit
TTCACAGTGGCTAAGTTCTG107120.10020786208883624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)