FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7347633
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG83969911.42815652333207No Hit
CAACGGAATCCCAAAAGCAGCTG4427976.026389723057752No Hit
CCCCCACTGCTAAATTTGACTG2703773.6797836799959933No Hit
AAGCTGCCAGTTGAAGAACTGT2601843.5410587327919076No Hit
TAGCAGCACGTAAATATTGGCG2469653.3611504548471594No Hit
AACATTCAACGCTGTCGGTGAGT2465163.355039643379031No Hit
TTCAAGTAATCCAGGATAGGCT2059742.8032701143347794No Hit
TAGCTTATCAGACTGATGTTGAC1972542.684592439497182No Hit
AACATTCAACGCTGTCGGTGAG1357351.8473296094129905No Hit
TAGCTTATCAGACTGATGTTGACT1307361.779294093757813No Hit
TATTGCACTTGTCCCGGCCTGTAA1017281.384500287371457No Hit
TATTGCACTTGTCCCGGCCTGTAT938891.2778128684434837No Hit
TGTAAACATCCTTGACTGGAAGCT848051.1541812172709225No Hit
TAGCTTATCAGACTGATGTTGA817041.1119771496480568No Hit
AGCAGCATTGTACAGGGCTATGA694470.9451615234457138No Hit
TGAGGTAGTAGGTTGTATAGTT589540.8023536287128112No Hit
TATTGCACTTGTCCCGGCCTGT571820.7782370186426023No Hit
CGCCTGTCTGAGCGTCGCT530540.7220556606460884No Hit
ACTGGACTTGGAGTCAGAAGGC497350.6768846511522826No Hit
TATTGCACTTGTCCCGGCCTGTA485900.6613014014173No Hit
TGAGGTAGTAGATTGTATAGTT416070.5662639927715497No Hit
CATAAAGTAGAAAGCACTACT383450.5218687433082191No Hit
TGTAAACATCCCCGACTGGAAGCT383210.5215421075059138No Hit
TTCAAGTAATTCAGGATAGGTT352930.48033155711506004No Hit
TGTAAACATCCTACACTCTCAGCT324010.44097194293726977No Hit
GCCTGTCTGAGCGTCGCT305410.4156576682586079No Hit
CCCATAAAGTAGAAAGCACTAC278840.37949636297839046No Hit
TGTCAGTTTGTCAAATACCCCAA275260.37462404559400286No Hit
TTCTCACTACTGCACTTGACTAG270410.36802328042241633No Hit
CTTCTCACTACTGCACTTGACTAG246420.33537331001698095No Hit
CAACGGAATCCCAAAAGCAGCTGT243610.3315489491649896No Hit
CATTGCACTTGTCTCGGTCTGA239180.32551979664743735No Hit
TACGCCTGTCTGAGCGTCGCT228950.31159694557417333No Hit
AACATTCAACGCTGTCGGTGAGTT221450.3013895767521323No Hit
TAGCTTATCAGACTGATGTTGACA216010.2939858318998785No Hit
TTAATGCTAATCGTGATAGGGGTT214860.29242070201383225No Hit
CCCATAAAGTAGAAAGCACT205100.2791375127200828No Hit
GCGTTCGCGCTTTCCCCTG198960.2707810801111052No Hit
GGAATACCGGGTGCTGTAGGCTTT191030.2599884888099338No Hit
TGTCAGTTTGTCAAATACCCCA182190.24795740342502137No Hit
CCCCCACTGCTAAATTTGACTGGCTT175530.2388932599110489No Hit
TGAGGTAGTAGTTTGTACAGTT174760.2378453033786527No Hit
CTACGCCTGTCTGAGCGTCGCT172500.23476948290694433No Hit
CTCCCACTGCTTCACTTGACTAG171120.23289132704368876No Hit
CCCCCCACTGCTAAATTTGACTGG165850.22571894921806793No Hit
CAACGGAATCCCAAAAGCAGCT164190.22345971825212282No Hit
TAGCAGCACGTAAATATTGGC157520.2143819649130543No Hit
ACTGGACTTGGAGTCAGAAGGCA150460.20477342839523965No Hit
TTCAAGTAATCCAGGATAGGCTT144300.19638977613606995No Hit
TGTAAACATCCTACACTCAGCT143610.1954506982044422No Hit
GGAATACCGGGTGCTGTAGGCTT141550.19264707423465488No Hit
TCGTACGACTCTTAGCGG141220.19219795000648507No Hit
CCCCCCACTGCTAAATTTGACTGGCT138150.1880197337019963No Hit
TTATAAAGCAATGAGACTGATT132780.18071125762541487No Hit
CAAAGAATTCTCCTTTTGGGCT131950.17958164214244232No Hit
AGCAGCATTGTACAGGGCTATGT128980.17553952408891407No Hit
TATTGCACTTGTCCCGGCCTGTAG127960.1741513219291165No Hit
TAGCAGCACGTAAATATTGGCGT126200.17175599271221084No Hit
CAACACCAGTCGATGGGCTGT123030.1674416781567615No Hit
CAAAGAATTCTCCTTTTGGGCTT120120.16348121905380958No Hit
AACGGAATCCCAAAAGCAGCTG115580.15730235846020071No Hit
ACGCCTGTCTGAGCGTCGCT114400.15569639909886626No Hit
TGAGGTAGTAGTTTGTGCTGTT111010.1510826683913037No Hit
CACTAGATTGTGAGCTCCTGGA108110.14713581911344784No Hit
TATTGCACTTGTCCCGGCCTGTT107970.14694528156210307No Hit
TGTAAACATCCCCGACTGGAAGC106950.14555707940230547No Hit
CCCCCCACTGCTAAATTTGACTGTA105990.14425053619308423No Hit
ACTGGACTTGGAGTCAGAAGG104730.1425356982309813No Hit
AACCGGGCGGAAACACCA103810.1412835943221443No Hit
CACTAGATTGTGAGCTCCTGGAA103040.14023563778974807No Hit
ACTGGACTTGGAGTCAGAAGGCT102800.13990900198744277No Hit
ACCACAGGGTAGAACCACGGAC102720.13980012338667433No Hit
AGCAGCATTGTACAGGGCTATG99770.13578522498333817No Hit
CCAAGTCCTTCTGATCGAGGCCC94680.12885782400944631No Hit
TCAGTGCACTACAGAACTTTGT94260.12828621135541202No Hit
TTCACAGTGGCTAAGTTCCG94240.1282589917052199No Hit
CTCCCACTGCTTCACTTGACTAGCCT93210.12685717972032626No Hit
ATCACATTGCCAGGGATTTCCA91630.12470682735514962No Hit
TGTCAGTTTGTCAAATACCCC87070.11850074711134864No Hit
CCTCGACACAAGGGTTTGT86540.11777942638125775No Hit
AGCTCGGTCTGAGGCCCCTCAGT85660.11658176177280492No Hit
GACCCGCGGGCGCTCTCCAGTCCT85510.11637761439636411No Hit
ACCCCCCACTGCTAAATTTGACTG85470.11632317509597989No Hit
GGGCTACGCCTGTCTGAGCGTCGCT84470.11496219258637441No Hit
TCGTTTCCCGGCCAATGCACCA81990.11158695596255284No Hit
CAGTGCAATAGTATTGTCAAAGC80750.10989933765064205No Hit
ATCACATTGCCAGGGATTTCC78700.10710932350595083No Hit
TGTAAACATCCTTGACTGGAAGC77690.1057347311712493No Hit
CTGCGTTCGCGCTTTCCCCTG77590.10559863292028875No Hit
CAAAGTGCTGTTCGTGCAGGTAG76820.10455067638789253No Hit
TTCAAGTAATCCAGGATAGGC76340.10389740478328191No Hit
CAAGTCCTTCTGATCGAGGCCC76250.10377491635741741No Hit
TGAGGTAGTAGGTTGTGTGGTT76060.10351632968059238No Hit
AACATTCAACGCTGTCGGTGA75590.10287666790107781No Hit
TCCTGTCTGAGCGTCGCT75310.10249559279838828No Hit
CCCCCCACTGCTAAATTTGACTGT73850.10050855833436427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)