FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6720682
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG96220314.317044014283073No Hit
CCCCCCACTGCTAAATTTGACTG4500416.696359089747142No Hit
TCGTACGACTCTTAGCGG3012204.481985608008235No Hit
GCCTGTCTGAGCGTCGCT2890014.300173702609348No Hit
CCCCCACTGCTAAATTTGACTG2408093.5831036195433734No Hit
TTCAAGTAATCCAGGATAGGCT1873612.787827187776479No Hit
TAGCAGCACGTAAATATTGGCG1521302.263609556292055No Hit
AACATTCAACGCTGTCGGTGAG1318211.9614229627290802No Hit
AAGCTGCCAGTTGAAGAACTGT1282631.9084819070445531No Hit
TGAGGTAGTAGATTGTATAGTT847931.2616725504941313No Hit
TATTGCACTTGTCCCGGCCTGT829771.2346514832869642No Hit
TAGCTTATCAGACTGATGTTGAC764901.1381285411212732No Hit
TGAGGTAGTAGGTTGTATAGTT637420.9484454107484925No Hit
TGAGAACTGAATTCCATGGGTT605280.9006228832133405No Hit
TAGCTTATCAGACTGATGTTGA604060.8988075912533876No Hit
AACCGGGCGGAAACACCA500110.744135788600026No Hit
AACATTCAACGCTGTCGGTGAGT484830.721400000773731No Hit
TATTGCACTTGTCCCGGCCTGTAA421040.6264840383758672No Hit
TAGCTTATCAGACTGATGTTGACT401460.597350090362853No Hit
CAACGGAATCCCAAAAGCAGCTGT393940.5861607497572419No Hit
CATAAAGTAGAAAGCACTACT385110.5730222022110256No Hit
TATTGCACTTGTCCCGGCCTGTAT380850.5666835597934852No Hit
TATTGCACTTGTCCCGGCCTGTA341310.5078502449602584No Hit
CATTGCACTTGTCTCGGTCTGA311920.46411956405614785No Hit
TCCTGTCTGAGCGTCGCT309510.46053361846312624No Hit
AGCAGCATTGTACAGGGCTATGA307720.45787019829237574No Hit
TGAGGTAGTAGTTTGTACAGTT303240.451204208144352No Hit
CAACGGAATCCCAAAAGCAGCT298580.44427038803502383No Hit
TTCAAGTAATTCAGGATAGGTT293890.4372919295988115No Hit
CCCATAAAGTAGAAAGCACT284940.42397482874505893No Hit
TTCTCACTACTGCACTTGACTAG279790.41631191596329065No Hit
TTAATGCTAATCGTGATAGGGGTT279510.4158952915790392No Hit
CCCATAAAGTAGAAAGCACTAC268720.39984037334306255No Hit
TGTAAACATCCTTGACTGGAAGCT258230.3842318383759267No Hit
AACGGAATCCCAAAAGCAGCTG253710.3775063304587243No Hit
AGGTCCCTGTTCGGGCGCCA192080.28580432759651475No Hit
ACTGGACTTGGAGTCAGAAGGC191040.2842568655978664No Hit
GGAATACCGGGTGCTGTAGGCTTT188580.2805965227933713No Hit
TGAGAACTGAATTCCATAGGCTGT174310.25936355863884053No Hit
TGAGGTAGTAGTTTGTGCTGTT170960.25437894547011747No Hit
CTCCCACTGCTTCACTTGACTAG170280.2533671433940782No Hit
TCGTTTCCCGGCCAATGCACCA170120.25312907231736304No Hit
CAGGTCCCTGTTCGGGCGCCA169850.25272732737540626No Hit
GGAATACCGGGTGCTGTAGGCTT167420.24911162289779518No Hit
CAACACCAGTCGATGGGCTGT166840.24824861524470285No Hit
CCCCCACTGCTAAATTTGACTGGCTT147170.21898075225103641No Hit
TAGCAGCACGTAAATATTGGC143120.2129545781216847No Hit
ACCGGGTGCTGTAGGCTT141740.21090121508501666No Hit
TGTAAACATCCCCGACTGGAAGCT141440.2104548318161758No Hit
AACCATCCTCTGCTACCA138480.2060505168969459No Hit
TTCACAGTGGCTAAGTTCCGC133180.19816441248075717No Hit
CTTCTCACTACTGCACTTGACTAG127510.1897277686996647No Hit
CCCATAAAGTAGAAAGCACTA120830.17978830124680797No Hit
ACCGGGTGCTGTAGGCTTT117370.17464001421284328No Hit
TGGCTCAGTTCAGCAGGAAC116380.1731669494256684No Hit
CACCCGGCTGTGTGCACATGTGC111030.16520644779800622No Hit
CCCCCCACTGCTAAATTTGACTGG106150.1579452799581947No Hit
TGAGGTAGTAGATTGTATAGT105910.15758817334312203No Hit
TTCACAGTGGCTAAGTTCTGC103120.15343680894290193No Hit
TATTGCACTTGTCCCGGCCTGTT102340.15227621244391568No Hit
TGATGTAGTAGATTGTATAGTT99700.148348039678116No Hit
ATCCCACCGCTGCCACCA94690.14089343908847346No Hit
TGTAAACATCCCCGACTGGA91290.13583442870827692No Hit
TGTAAACATCCTACACTCTCAGCT88890.13226336255754995No Hit
TGTCAGTTTGTCAAATACCCCA85950.12788880652290943No Hit
CGCCTGTCTGAGCGTCGCT84430.12562713129411568No Hit
TAGCTTATCAGACTGATGTTGACA84130.12518074802527482No Hit
TTCAAGTAATCCAGGATAGGC84130.12518074802527482No Hit
AGCTCGGTCTGAGGCCCCTCAGT83480.1242135842761196No Hit
GAATACCGGGTGCTGTAGGCTTT82340.12251732785452428No Hit
TGAGAACTGAATTCCATGGGTTG80430.11967535437623741No Hit
CACTAGATTGTGAGCTCCTGGA78860.1173392819359702No Hit
CTCGTACGACTCTTAGCGG77750.11568766384125898No Hit
ACCGGGTGCTGTAGGCTTA77650.11553886941831201No Hit
TGATGTAGTAGGTTGTATAGTT76640.11403604574654774No Hit
GGGAATACCGGGTGCTGTAGGCTTT76610.11399140741966365No Hit
GGGAATACCGGGTGCTGTAGGCTT76550.11390213076589548No Hit
TCCTTCTGATCGAGGCCC75200.11189340605611156No Hit
ATCACGTCGGGGTCACCA72940.10853065209751034No Hit
TCGTTTCCCGGCCAACGCACCA72910.10848601377062626No Hit
CTCCCACTGCTTCACTTGACTAGCCT72630.10806938938637478No Hit
ACCCCCCACTGCTAAATTTGACTG70970.1055994019654553No Hit
GAATACCGGGTGCTGTAGGCTT70570.10500422427366747No Hit
TCGGACGACTCTTAGCGG68340.10168610864195032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)