FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5857277
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG80110013.677003836424332No Hit
CAACGGAATCCCAAAAGCAGCTG5717409.761191079062847No Hit
CCCCCACTGCTAAATTTGACTG1834173.1314380385288247No Hit
AACATTCAACGCTGTCGGTGAGT1790603.057051937273924No Hit
TTCAAGTAATCCAGGATAGGCT1641672.8027870288531687No Hit
AAGCTGCCAGTTGAAGAACTGT1447292.470926336589511No Hit
TAGCAGCACGTAAATATTGGCG1441752.4614680166227414No Hit
TATTGCACTTGTCCCGGCCTGTAA1346852.2994473370475736No Hit
TATTGCACTTGTCCCGGCCTGTAT1294352.209815243499667No Hit
AACATTCAACGCTGTCGGTGAG1194162.038763063450815No Hit
TAGCTTATCAGACTGATGTTGACT789741.3483057058766386No Hit
TAGCTTATCAGACTGATGTTGAC690571.1789949493595746No Hit
TATTGCACTTGTCCCGGCCTGTA504690.8616461198608159No Hit
TATTGCACTTGTCCCGGCCTGT476860.8141325738905638No Hit
TGAGGTAGTAGGTTGTATAGTT438430.7485218814134964No Hit
TGTAAACATCCTTGACTGGAAGCT428260.7311588644347877No Hit
AGCAGCATTGTACAGGGCTATGA426060.7274028528956373No Hit
TAGCTTATCAGACTGATGTTGA411640.702783904534479No Hit
TGTAAACATCCCCGACTGGAAGCT344250.5877304419784142No Hit
CAACGGAATCCCAAAAGCAGCTGT334640.5713235006642164No Hit
ACTGGACTTGGAGTCAGAAGGC314860.5375535423713101No Hit
CCCCCACTGCTAAATTTGACTGGCTT293090.5003861009134449No Hit
TGAGGTAGTAGATTGTATAGTT289410.4941033179752298No Hit
CCCATAAAGTAGAAAGCACTAC243510.41573925904477455No Hit
TTCAAGTAATTCAGGATAGGTT237610.40566631900796224No Hit
GGAATACCGGGTGCTGTAGGCTTT233640.39888842545776815No Hit
CATAAAGTAGAAAGCACTACT208850.356565004523433No Hit
CAACGGAATCCCAAAAGCAGCT206280.3521773001345164No Hit
CCCCCCACTGCTAAATTTGACTGGCT206170.35198949955755887No Hit
TCGTACGACTCTTAGCGG200490.3422921606746616No Hit
CTCCCACTGCTTCACTTGACTAG196950.3362483966525742No Hit
CAAAGAATTCTCCTTTTGGGCT190370.32501450759456996No Hit
CTTCTCACTACTGCACTTGACTAG187630.3203365659503554No Hit
CACTAGATTGTGAGCTCCTGGAA182070.31084410042413907No Hit
TTCTCACTACTGCACTTGACTAG180790.3086587846195425No Hit
TATTGCACTTGTCCCGGCCTGTAG169610.2895714168887693No Hit
ACCCCCCACTGCTAAATTTGACTG162360.2771936515892965No Hit
AGCTCGGTCTGAGGCCCCTCAGT150690.2572697176520762No Hit
CCCCCCACTGCTAAATTTGACTGG149450.2551526929663733No Hit
CACTAGATTGTGAGCTCCTGGA146440.2500137862696266No Hit
CATTGCACTTGTCTCGGTCTGA145350.24815285327977488No Hit
GGAATACCGGGTGCTGTAGGCTT143260.24458464231758206No Hit
AACATTCAACGCTGTCGGTGAGTT143260.24458464231758206No Hit
TGTAAACATCCTACACTCTCAGCT140050.23910428002636722No Hit
TCGTTTCCCGGCCAATGCACCA135560.2314386019305558No Hit
CTCCCACTGCTTCACTTGACTAGCCT132620.22641920469187302No Hit
TATTGCACTTGTCCCGGCCTGTAAA130410.22264612037299927No Hit
CCCCCCACTGCTAAATTTGACTGTA127350.21742184977763557No Hit
GACCCGCGGGCGCTCTCCAGTCCT125360.21402436661267682No Hit
TATTGCACTTGTCCCGGCCTGTTT121810.20796352981086605No Hit
TAGCTTATCAGACTGATGTTGACA119840.2046001922053541No Hit
TATTGCACTTGTCCCGGCCTGTT119480.20398557213531132No Hit
TATTGCACTTGTCCCGGCCTGTAAT117980.20142465517679975No Hit
TCGTTTCCCGGGCGGCGCACCA111730.19075416784966803No Hit
CCCCCCACTGCTAAATTTGACTGT105900.18080073727091958No Hit
TAGCAGCACGTAAATATTGGC103340.17643010566172643No Hit
ACTGGACTTGGAGTCAGAAGGCA102620.17520086552164088No Hit
CAAAGAATTCTCCTTTTGGGCTT102140.17438137209491714No Hit
CCCCCCACTGCTAAATTTGACTGA99820.17042048719908587No Hit
CCCATAAAGTAGAAAGCACT99550.16995952214655377No Hit
TGAGGTAGTAGTTTGTACAGTT97100.16577669111431811No Hit
TGAATACCGGGTGCTGTAGGCTTT96450.16466696043229645No Hit
AACGGAATCCCAAAAGCAGCTG95370.1628231002221681No Hit
TTCAAGTAATCCAGGATAGGCTT94270.1609450944525929No Hit
TGTAAACATCCCCGACTGGAAGC89470.15275016018535575No Hit
AGCAGCATTGTACAGGGCTATGT86110.14701370619828977No Hit
CACCCGGCTGTGTGCACATGTGC85950.1467405417227152No Hit
TGATGTAGTAGGTTGTATAGTT85520.14600641219460853No Hit
CGGGCCGCCGGTGAAATACCACTACT85250.14554544714207643No Hit
ACTGGACTTGGAGTCAGAAGGCT83760.14300160296328823No Hit
TGTCAGTTTGTCAAATACCCCAA82280.14047483156422344No Hit
ATGCACCTGGGCAAGGATTCTGA78070.13328719130066752No Hit
CTACGGGGATGATTTTACGAACTGAA71880.1227191406518763No Hit
CTACGCCTGTCTGAGCGTCGCT70980.12118259047676933No Hit
TTCAAGTAATCCAGGATAGGC70410.1202094420325349No Hit
CCCCCACTGCTAAATTTGACTGGCTA70170.11979969531917306No Hit
ACTTGACTTGGAGTCAGAAGGC65300.11148525159387203No Hit
CAACGGAATCCCAAAAGCAGCTGA65260.11141696047497839No Hit
ATCACATTGCCAGGGATTTCCA64950.11088770430355266No Hit
TGAGGTAGTAGTTTGTGCTGTT63580.10854873348144539No Hit
ATCACATTGCCAGGGATTTCC63530.10846336958282835No Hit
TGTCAGTTTGTCAAATACCCCA62620.10690974662799796No Hit
TGAATACCGGGTGCTGTAGGCTT60570.10340982678469876No Hit
TATTGCACTTGTCCCGGCCTGA60320.1029830072916135No Hit
CGCCTGTCTGAGCGTCGCT59390.10139523877733629No Hit
AACCGGGCGGAAACACCA59250.10115621986120854No Hit
CAACACCAGTCGATGGGCTGT59200.1010708559625915No Hit
TTCACAGTGGCTAAGTTCCG58960.10066110924922964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)