FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9152531
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG161756717.673439183106836No Hit
CAACGGAATCCCAAAAGCAGCTG7175877.840312149721209No Hit
CCCCCACTGCTAAATTTGACTG4696815.131706191434915No Hit
TTCAAGTAATCCAGGATAGGCT2847093.110713309793761No Hit
AACATTCAACGCTGTCGGTGAGT2369352.588737476005271No Hit
TAGCAGCACGTAAATATTGGCG2249172.4574295350652187No Hit
AAGCTGCCAGTTGAAGAACTGT1943182.123106712230748No Hit
AACATTCAACGCTGTCGGTGAG1721161.8805290033980766No Hit
TAGCTTATCAGACTGATGTTGAC1170821.2792308488220363No Hit
TATTGCACTTGTCCCGGCCTGTAT1156751.263858051942135No Hit
TAGCTTATCAGACTGATGTTGACT1118541.222110037103398No Hit
TATTGCACTTGTCCCGGCCTGTAA1067821.1666936719471368No Hit
TAGCTTATCAGACTGATGTTGA877450.9586965616396165No Hit
TGTAAACATCCTTGACTGGAAGCT651730.7120762551910504No Hit
CATAAAGTAGAAAGCACTACT622970.6806532531820979No Hit
TATTGCACTTGTCCCGGCCTGT555120.6065207536581957No Hit
AACCGGGCGGAAACACCA540100.5901099925255648No Hit
TATTGCACTTGTCCCGGCCTGTA506530.5534316136159495No Hit
AGCAGCATTGTACAGGGCTATGA506330.5532130948259012No Hit
TGAGGTAGTAGGTTGTATAGTT495530.5414130801632904No Hit
CTCCCACTGCTTCACTTGACTAG489810.5351634427679076No Hit
TTCTCACTACTGCACTTGACTAG455630.4978185815486449No Hit
TTCAAGTAATTCAGGATAGGTT435510.47583559126978103No Hit
CTTCTCACTACTGCACTTGACTAG432870.47295114324114285No Hit
CCCATAAAGTAGAAAGCACTAC421770.46082335039345945No Hit
CAACGGAATCCCAAAAGCAGCTGT388220.42416682336284905No Hit
TGAGGTAGTAGATTGTATAGTT378100.41310977258640263No Hit
TGTAAACATCCCCGACTGGAAGCT363660.3973327159449118No Hit
ACTGGACTTGGAGTCAGAAGGC345350.3773273207159855No Hit
CCCATAAAGTAGAAAGCACT340230.37173323969074784No Hit
CATTGCACTTGTCTCGGTCTGA306330.33469430477755274No Hit
CCCCCACTGCTAAATTTGACTGGCTT299110.3268057764568074No Hit
TGTAAACATCCTACACTCTCAGCT298750.32641244263472036No Hit
GCCTGTCTGAGCGTCGCT296550.32400873594418855No Hit
GGAATACCGGGTGCTGTAGGCTTT285170.31157501679043753No Hit
CAAAGAATTCTCCTTTTGGGCT284430.31076649726725863No Hit
CAACGGAATCCCAAAAGCAGCT282090.30820982742369296No Hit
GACCCGCGGGCGCTCTCCAGTCCT272490.29772092550137225No Hit
ACCCCCCACTGCTAAATTTGACTG264500.2889910998389407No Hit
TCGTACGACTCTTAGCGG259520.28354998196673686No Hit
CCCCCCACTGCTAAATTTGACTGG249660.2727770056173533No Hit
CCCCCCACTGCTAAATTTGACTGGCT214950.2348530696044624No Hit
GGAATACCGGGTGCTGTAGGCTT200620.21919619829749828No Hit
CACTAGATTGTGAGCTCCTGGAA190890.2085652591616461No Hit
ACCGGGTGCTGTAGGCTTA190480.20811729564204698No Hit
TGTCAGTTTGTCAAATACCCCAA185030.2021626586132295No Hit
CACTAGATTGTGAGCTCCTGGA182150.19901598803653328No Hit
CAAAGAATTCTCCTTTTGGGCTT180230.19691820765206916No Hit
CTCCCACTGCTTCACTTGACTAGCCT179170.1957600580648129No Hit
AGCTCGGTCTGAGGCCCCTCAGT177410.19383709271238742No Hit
TTCAAGTAATCCAGGATAGGCTT167340.18283467163345307No Hit
TGTCAGTTTGTCAAATACCCCA163280.1783987401954716No Hit
AACATTCAACGCTGTCGGTGAGTT162420.1774591093982637No Hit
TGAGGTAGTAGTTTGTACAGTT153490.16770224542260495No Hit
TAGCAGCACGTAAATATTGGC147910.16160557118025604No Hit
CAACACCAGTCGATGGGCTGT143940.15726797319779634No Hit
TATTGCACTTGTCCCGGCCTGTAG142640.15584760106248205No Hit
AACGGAATCCCAAAAGCAGCTG137070.14976185275963555No Hit
CACCCGGCTGTGTGCACATGTGC135120.14763129455666418No Hit
CTACGCCTGTCTGAGCGTCGCT135080.1475875907986545No Hit
TAGCTTATCAGACTGATGTTGACA134100.14651684872741758No Hit
CCCCCCACTGCTAAATTTGACTGT133400.14575203296224837No Hit
CGCCTGTCTGAGCGTCGCT132180.14441906834295345No Hit
TCAGTGCACTACAGAACTTTGT131220.14337017815072137No Hit
ACCGGGTGCTGTAGGCTT123400.13482609345983096No Hit
TTCAAGTAATCCAGGATAGGC123000.13438905587973426No Hit
CCCATAAAGTAGAAAGCACTA122170.1334822029010336No Hit
TGTAAACATCCCCGACTGGAAGC121000.13220386797925077No Hit
TATTGCACTTGTCCCGGCCTGTT120750.13193071949169033No Hit
ACCGGGTGCTGTAGGCTTT118230.12917738273708113No Hit
TGAATACCGGGTGCTGTAGGCTTT114860.12549534112476646No Hit
TCCTGTCTGAGCGTCGCT113450.12395478365492563No Hit
TTCACAGTGGCTAAGTTCCG110780.12103755780778017No Hit
TGTAAACATCCTACACTCAGCT110110.12030551986111819No Hit
TATTGCACTTGTCCCGGCCTGTTT108840.11891792554431119No Hit
AGCAGCATTGTACAGGGCTATGT108620.118677554875258No Hit
ATCACATTGCCAGGGATTTCC108050.1180547763236202No Hit
ACTGGACTTGGAGTCAGAAGGCT106260.11609903315268749No Hit
CCCCCCACTGCTAAATTTGACTGA105100.11483162417040707No Hit
ACTGGACTTGGAGTCAGAAGGCA103890.11350958549061457No Hit
CCCCCCACTGCTAAATTTGACTGTA99610.1088332833835799No Hit
ATACCGGGTGCTGTAGGCTTA99080.10825420858995179No Hit
ATCACATTGCCAGGGATTTCCA97020.1060034650524538No Hit
TGATGTAGTAGGTTGTATAGTT94550.1033047579953567No Hit
CTCCCACTGCTTCACTTGACTA93990.10269290538322132No Hit
TTCACAGTGGCTAAGTTCTGC93790.10247438659317298No Hit
TGAGGTAGTAGTTTGTGCTGTT93660.10233234937964154No Hit
ATCCCGGACGAGCCCCCA92220.10075901409129344No Hit
TATTGCACTTGTCCCGGCCTGTAAT91980.10049679154323542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)