FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9549127
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG128807513.488929406845255No Hit
CAACGGAATCCCAAAAGCAGCTG4548894.763671066475501No Hit
CCCCCACTGCTAAATTTGACTG4211984.410853473830644No Hit
AACATTCAACGCTGTCGGTGAGT2942193.081108880424357No Hit
AAGCTGCCAGTTGAAGAACTGT2149142.2506141137299776No Hit
TAGCAGCACGTAAATATTGGCG2133522.2342565974879167No Hit
TTCAAGTAATCCAGGATAGGCT1919942.010592172457231No Hit
AACATTCAACGCTGTCGGTGAG1855721.943339951390321No Hit
CGCCTGTCTGAGCGTCGCT1768461.851959870258297No Hit
TAGCTTATCAGACTGATGTTGAC1603251.6789492903382688No Hit
TAGCTTATCAGACTGATGTTGACT906710.9495213541510129No Hit
TATTGCACTTGTCCCGGCCTGTAT897930.9403267963657831No Hit
TATTGCACTTGTCCCGGCCTGTAA882310.9239692801237223No Hit
TATTGCACTTGTCCCGGCCTGT832600.8719121653738608No Hit
TGAGGTAGTAGGTTGTATAGTT785340.8224207301882152No Hit
TAGCTTATCAGACTGATGTTGA779510.8163154600415304No Hit
AGCAGCATTGTACAGGGCTATGA697790.73073695637308No Hit
TATTGCACTTGTCCCGGCCTGTA652410.683214287546914No Hit
ACCGGGTGCTGTAGGCTT591190.6191037149259823No Hit
TGTAAACATCCTTGACTGGAAGCT582600.6101081282089975No Hit
GCGTTCGCGCTTTCCCCTG572870.5999187150825411No Hit
TGAGGTAGTAGATTGTATAGTT539350.5648160297794762No Hit
ACTGGACTTGGAGTCAGAAGGC511910.5360804186602608No Hit
GCCTGTCTGAGCGTCGCT493300.5165917261337084No Hit
TACGCCTGTCTGAGCGTCGCT454130.4755722695907175No Hit
ACCGGGTGCTGTAGGCTTT432870.45330845427021754No Hit
CATAAAGTAGAAAGCACTACT366300.38359527525395776No Hit
ACCGGGTGCTGTAGGCTTA366300.38359527525395776No Hit
TTCAAGTAATTCAGGATAGGTT339210.35522618978677317No Hit
TCGTACGACTCTTAGCGG334490.35028332956510055No Hit
CATTGCACTTGTCTCGGTCTGA320380.3355071097075157No Hit
TGTAAACATCCCCGACTGGAAGCT308870.32345365183644537No Hit
CTACGCCTGTCTGAGCGTCGCT298920.3130338511572838No Hit
ACGCCTGTCTGAGCGTCGCT267140.2797533219528864No Hit
CAACGGAATCCCAAAAGCAGCT246010.2576256447317121No Hit
AACATTCAACGCTGTCGGTGAGTT239170.25046268627488144No Hit
TTCTCACTACTGCACTTGACTAG236460.24762473051201436No Hit
TGAGGTAGTAGTTTGTACAGTT232570.24355105969372906No Hit
TGTCAGTTTGTCAAATACCCCAA228370.2391527518693594No Hit
CTCCCACTGCTTCACTTGACTAG224630.23523616347337303No Hit
CCCATAAAGTAGAAAGCACTAC222170.23266001174767076No Hit
TTAATGCTAATCGTGATAGGGGTT217800.22808367717802894No Hit
GACCCGCGGGCGCTCTCCAGTCCT217580.22785328962532386No Hit
CAACACCAGTCGATGGGCTGT217410.22767526288005177No Hit
CAACGGAATCCCAAAAGCAGCTGT209040.21891006371577212No Hit
TGAGGTAGTAGTTTGTGCTGTT208180.218009457827925No Hit
CCCCCCACTGCTAAATTTGACTGG204390.2140405086245057No Hit
CCCATAAAGTAGAAAGCACT201780.2113072744765045No Hit
AACCGGGCGGAAACACCA201540.21105594260082622No Hit
CTTCTCACTACTGCACTTGACTAG195260.20447942518724485No Hit
TGTAAACATCCTACACTCTCAGCT186200.19499164688039022No Hit
CCCCCACTGCTAAATTTGACTGGCTT175860.1841634319032515No Hit
CCTCGACACAAGGGTTTGT173550.18174436259984814No Hit
TAGCTTATCAGACTGATGTTGACA166520.17438243307477216No Hit
ACTGGACTTGGAGTCAGAAGGCA151820.1589883556894782No Hit
TAGCAGCACGTAAATATTGGC150780.1578992508948724No Hit
ATCCCGGACGAGCCCCCA150060.15714525526783757No Hit
TTATAAAGCAATGAGACTGATT148450.15545923726849584No Hit
ACCACAGGGTAGAACCACGGAC142470.14919688469951234No Hit
GGAATACCGGGTGCTGTAGGCTTT142030.14873610959410216No Hit
AGCAGCATTGTACAGGGCTATG139170.14574107140893613No Hit
TGTCAGTTTGTCAAATACCCCA138160.14468338309879006No Hit
TATTGCACTTGTCCCGGCCTGTT137910.14442157906162523No Hit
AGCAGCATTGTACAGGGCTATGT137720.1442226079933799No Hit
ACTGGACTTGGAGTCAGAAGG136360.14279839403120306No Hit
AACGGAATCCCAAAAGCAGCTG135290.14167787275213745No Hit
CCCCCCACTGCTAAATTTGACTGGCT135060.14143701303794579No Hit
TACCTGGTTGATCCTGCC134900.14126945845416025No Hit
TTCACAGTGGCTAAGTTCCG132920.13919597047981452No Hit
GGAATACCGGGTGCTGTAGGCTT129650.13577157367369813No Hit
TCGTTTCCCGGCCAATGCACCA126610.1325880365817734No Hit
TATTGCACTTGTCCCGGCCTGTAG126090.13204348418447048No Hit
CTGCGTTCGCGCTTTCCCCTG123870.12971866433444648No Hit
TGAGGTAGTAGGTTGTGTGGTT121630.1273729001614493No Hit
ACCCCCCACTGCTAAATTTGACTG121030.12674457047225365No Hit
TTCAAGTAATCCAGGATAGGCTT119280.12491194221209961No Hit
CACCCGGCTGTGTGCACATGTGC117170.12270231613842814No Hit
CCAAGTCCTTCTGATCGAGGCCC116610.12211587509517885No Hit
GGGCTACGCCTGTCTGAGCGTCGCT115670.12113149191543898No Hit
CTCCCACTGCTTCACTTGACTAGCCT114610.12002144279785994No Hit
TAGCAGCACGTAAATATTGGCGT113590.11895328232622732No Hit
TGTAAACATCCTACACTCAGCT113140.11848203505933055No Hit
ACTGGACTTGGAGTCAGAAGGCT112070.11736151378026494No Hit
CAAGTCCTTCTGATCGAGGCCC109740.11492150015388841No Hit
GTGCGGTAACGCGACCGATCCCGGA109170.11432458694915253No Hit
TGAAAAGAACTTTGAAGAGAG107570.1126490411112974No Hit
CACTAGATTGTGAGCTCCTGGA107400.11247101436602529No Hit
ATCCCACCGCTGCCACCA102210.10703596255448272No Hit
CTCGACACAAGGGTTTGT100280.10501483538756999No Hit
GCTTGACTAGCTTGCTGTTT98670.10332881738822827No Hit
CAAAGTGCTGTTCGTGCAGGTAG97710.10232348988551518No Hit
TGTAAACATCCCCGACTGGAAGC96490.10104588618415064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)