FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10147538
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9839729.696657455236926No Hit
CCCCCCACTGCTAAATTTGACTG9598409.458846076752804No Hit
CCCCCACTGCTAAATTTGACTG4861994.791300116343492No Hit
TGAGGTAGTAGATTGTATAGTT4152154.091780686113223No Hit
TTCAAGTAATCCAGGATAGGCT4130844.07078051838781No Hit
TAGCAGCACGTAAATATTGGCG2995342.9517898824325663No Hit
TAGCTTATCAGACTGATGTTGA2647042.608553917216176No Hit
TAGCTTATCAGACTGATGTTGAC2619862.581769095124354No Hit
AACATTCAACGCTGTCGGTGAG1881231.853878251059518No Hit
AAGCTGCCAGTTGAAGAACTGT1870401.8432057115726004No Hit
TGAGGTAGTAGGTTGTATAGTT1731601.7064237650551297No Hit
TAGCTTATCAGACTGATGTTGACT1421601.400930945023315No Hit
TCGTACGACTCTTAGCGG1273721.2552010152610416No Hit
TGAGGTAGTAGTTTGTACAGTT1185411.1681749799803658No Hit
TTCAAGTAATTCAGGATAGGTT1057121.0417502255226836No Hit
CATAAAGTAGAAAGCACTACT1046921.031698526282927No Hit
TGAGAACTGAATTCCATGGGTT978790.9645590881256123No Hit
TATTGCACTTGTCCCGGCCTGT916430.9031057582637286No Hit
TGTAAACATCCTTGACTGGAAGCT821470.8095264092630153No Hit
TTCTCACTACTGCACTTGACTAG645650.6362627072694874No Hit
TATTGCACTTGTCCCGGCCTGTAT634000.62478209000055No Hit
AACATTCAACGCTGTCGGTGAGT624140.6150654474021187No Hit
TATTGCACTTGTCCCGGCCTGTAA618910.609911487889969No Hit
GCCTGTCTGAGCGTCGCT602470.593710513821185No Hit
CATTGCACTTGTCTCGGTCTGA523230.5156226071782141No Hit
TATTGCACTTGTCCCGGCCTGTA508550.501156043958643No Hit
AGCAGCATTGTACAGGGCTATGA503740.49641597794460096No Hit
TGATGTAGTAGATTGTATAGTT479120.4721539352698162No Hit
TGAGGTAGTAGATTGTATAGT466260.45948091054204476No Hit
TTAATGCTAATCGTGATAGGGGTT454600.44799043866600946No Hit
CAACGGAATCCCAAAAGCAGCTGT391150.3854629566304654No Hit
TGAGGTAGTAAGTTGTATTGTT370150.36476828172508446No Hit
TTCACAGTGGCTAAGTTCCGC368370.363014161661676No Hit
TGAGAACTGAATTCCATAGGCTGT350890.34578830845472075No Hit
CAACGGAATCCCAAAAGCAGCT342400.33742174702868816No Hit
TGTCAGTTTGTCAAATACCCCA336670.33177505716164846No Hit
TGTAAACATCCCCGACTGGAAGCT305710.3012651935868582No Hit
TAGCTTATCAGACTGATGTTGACA302310.29791462717360606No Hit
CTTCTCACTACTGCACTTGACTAG301990.29759927974647643No Hit
CCCCCACTGCTAAATTTGACTGGCTT298490.29415016726224624No Hit
TAGCAGCACGTAAATATTGGC295040.2907503278135051No Hit
TGAGGTAGTAGTTTGTGCTGTT273630.2696516140171143No Hit
CAAAGAATTCTCCTTTTGGGCT270500.26656712199550275No Hit
CTCCCACTGCTTCACTTGACTAG265580.26171865530338495No Hit
AACGGAATCCCAAAAGCAGCTG252140.2484740633639411No Hit
TTCACAGTGGCTAAGTTCTGC241440.2379296337692946No Hit
CAACACCAGTCGATGGGCTGT237010.23356404282496895No Hit
ACCGGGTGCTGTAGGCTT235570.23214497940288573No Hit
ACTGGACTTGGAGTCAGAAGGC235090.2316719582621913No Hit
TTATAAAGCAATGAGACTGATT218640.21546112958630953No Hit
TTCACAGTGGCTAAGTTCTG215830.21269198499182757No Hit
TCAGTGCACTACAGAACTTTGT213730.21062251750128946No Hit
TGTAAACATCCCCGACTGGA203550.2005905274757286No Hit
TGAGGTAGTAGATTGTATAGTTA199060.19616580888881618No Hit
TGATGTAGTAGGTTGTATAGTT198670.19578147921200198No Hit
ACCGGGTGCTGTAGGCTTT196110.19325869979496504No Hit
TCGTTTCCCGGCCAATGCACCA195280.19244076740584762No Hit
TGTAAACATCCTACACTCTCAGCT191300.188518633780923No Hit
ACCCCCCACTGCTAAATTTGACTG184800.18211313916735272No Hit
TTCAAGTAATCCAGGATAGGC177210.17463349238012216No Hit
TTCACAGTGGCTAAGTTCCG165620.1632120027537714No Hit
TGAGGTAGTAGTTTGTGCTGTTA164100.16171410247490572No Hit
CCCCCCACTGCTAAATTTGACTGG163620.1612410813342113No Hit
TAGCTTATCAGACTGATGTTG158980.15666854364083188No Hit
CTCCCACTGCTTCACTTGACTAGCCT157680.15538744471811783No Hit
TGTCAGTTTGTCAAATACC157280.1549932604342058No Hit
TCAGTGCACTACAGAACTTTGTC155820.15355448779792694No Hit
TGGCTCAGTTCAGCAGGAAC154950.15269713698041829No Hit
AACCGGGCGGAAACACCA153190.15096272613120543No Hit
TTCAAGTAATCCAGGATAGGCTT152570.1503517404911418No Hit
CAAAGAATTCTCCTTTTGGGCTT151630.14942540742394855No Hit
TGTCAGTTTGTCAAATACCCCAA144680.14257645549097722No Hit
TATTGCACTTGTCCCGGCCTGTT141550.13949196346936565No Hit
CAAAGAATTCTCCTTTTGGGC140640.13859519422346583No Hit
TGATGTAGTAGTTTGTACAGTT133590.1316476962195165No Hit
TGTCAGTTTGTCAAATACCCC132230.13030746965421564No Hit
CCCATAAAGTAGAAAGCACTAC132050.13013008672645524No Hit
CACCCGGCTGTGTGCACATGTGC130510.12861247723339395No Hit
CCCATAAAGTAGAAAGCACT128800.12692733941967008No Hit
GGAATACCGGGTGCTGTAGGCTTT127610.12575464117503182No Hit
GACCCGCGGGCGCTCTCCAGTCCT124660.12284753208118067No Hit
CAAAGTGCTGTTCGTGCAGGTAG124490.12268000376051806No Hit
ATCACATTGCCAGGGATTTCC120410.11865932406461548No Hit
ACCGGGTGCTGTAGGCTTA119180.11744720739158601No Hit
TGAGAACTGAATTCCATGGGTTG114720.113052052625967No Hit
TAGCAGCACGTAAATATTGGCGT110320.10871602550293481No Hit
AGCTACATTGTCTGCTGGGTTT110160.10855835178937No Hit
TGAGGTAGTAGGTTGTATAGT106710.10515851234062883No Hit
TGTAAACATCCCCGACTGGAAGC105410.10387741341791477No Hit
CACTAGATTGTGAGCTCCTGGA104730.10320730013526433No Hit
GGAATACCGGGTGCTGTAGGCTT104130.1026160237093963No Hit
TAGCAGCACATCATGGTTTACA104050.1025371868526139No Hit
TGATAACTGAATTCCATGGGTT103640.10213314796160408No Hit
TCCCACTGCTTCACTTGACTAG102400.10091117668147682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)