FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8918553
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG91983510.31372465914594No Hit
CAACGGAATCCCAAAAGCAGCTG5930696.649834339718562No Hit
CCCCCACTGCTAAATTTGACTG3603134.040038782075971No Hit
AACATTCAACGCTGTCGGTGAGT3479863.9018212932075413No Hit
TAGCAGCACGTAAATATTGGCG2802533.1423595285019887No Hit
AAGCTGCCAGTTGAAGAACTGT2731603.0628286898110044No Hit
TTCAAGTAATCCAGGATAGGCT2707373.0356606054816293No Hit
AACATTCAACGCTGTCGGTGAG2079312.33144322851476No Hit
TAGCTTATCAGACTGATGTTGAC1152821.2926087897891059No Hit
TGAGGTAGTAGGTTGTATAGTT1136161.2739286294536794No Hit
TAGCTTATCAGACTGATGTTGACT998031.119049244871898No Hit
TAGCTTATCAGACTGATGTTGA990011.1100567547224307No Hit
TATTGCACTTGTCCCGGCCTGTAA752340.8435673365399073No Hit
TATTGCACTTGTCCCGGCCTGTAT718460.8055791113199642No Hit
CGCCTGTCTGAGCGTCGCT718300.805399710020224No Hit
TATTGCACTTGTCCCGGCCTGT702430.7876053436022638No Hit
TGTAAACATCCTTGACTGGAAGCT674840.756669831978349No Hit
CATAAAGTAGAAAGCACTACT673760.7554588732051041No Hit
GCCTGTCTGAGCGTCGCT577650.6476947549675379No Hit
AGCAGCATTGTACAGGGCTATGA565800.6344078462055448No Hit
TGAGGTAGTAGATTGTATAGTT564540.6329950609700924No Hit
TCGTACGACTCTTAGCGG525490.5892099312523007No Hit
TATTGCACTTGTCCCGGCCTGTA470260.5272828450983024No Hit
ACTGGACTTGGAGTCAGAAGGC449340.5038261251572985No Hit
AACCGGGCGGAAACACCA404610.4536722492987371No Hit
CCCATAAAGTAGAAAGCACTAC392830.4404638286053803No Hit
TTCAAGTAATTCAGGATAGGTT392560.44016108891206907No Hit
TGTAAACATCCCCGACTGGAAGCT390890.43828858784603286No Hit
ACCGGGTGCTGTAGGCTT390290.4376158329720079No Hit
CCCATAAAGTAGAAAGCACT339970.38119412420378057No Hit
CAACGGAATCCCAAAAGCAGCTGT333640.3740965602828172No Hit
CTACGCCTGTCTGAGCGTCGCT302520.33920300748338883No Hit
TTCTCACTACTGCACTTGACTAG301060.33756597062326144No Hit
TGAGGTAGTAGTTTGTACAGTT295640.33148875159456925No Hit
TACGCCTGTCTGAGCGTCGCT279120.31296556739641507No Hit
CATTGCACTTGTCTCGGTCTGA269460.3021342139246131No Hit
GACCCGCGGGCGCTCTCCAGTCCT261980.2937472031617685No Hit
ACCGGGTGCTGTAGGCTTT258380.2897106739176187No Hit
CTCCCACTGCTTCACTTGACTAG255090.2860217346917151No Hit
AACATTCAACGCTGTCGGTGAGTT240670.2698531925526484No Hit
ACCGGGTGCTGTAGGCTTA232700.26091676530935004No Hit
TGTAAACATCCTACACTCTCAGCT230060.2579566438636402No Hit
CTTCTCACTACTGCACTTGACTAG227280.25483954628065786No Hit
TGTCAGTTTGTCAAATACCCCAA216920.2432233121224934No Hit
CCCCCACTGCTAAATTTGACTGGCTT209530.23493721459075254No Hit
ACGCCTGTCTGAGCGTCGCT203350.2280078393882954No Hit
TGAGGTAGTAGTTTGTGCTGTT200330.22462163985570305No Hit
CAACACCAGTCGATGGGCTGT199250.22341068108245812No Hit
CCCCCCACTGCTAAATTTGACTGG198140.2221660845655119No Hit
AACGGAATCCCAAAAGCAGCTG185350.20782519316754636No Hit
CAACGGAATCCCAAAAGCAGCT184520.20689454892514514No Hit
TCCTGTCTGAGCGTCGCT164240.18415543418310124No Hit
ACCCCCCACTGCTAAATTTGACTG163120.18289962508492127No Hit
TAGCAGCACGTAAATATTGGC156550.1755329592143479No Hit
CCCCCCACTGCTAAATTTGACTGGCT155310.17414259914136296No Hit
TTCACAGTGGCTAAGTTCCG154790.173559544917208No Hit
AGCTCGGTCTGAGGCCCCTCAGT153550.17216918484422306No Hit
TGAGGTAGTAGGTTGTGTGGTT144340.1618423975279398No Hit
TTCAAGTAATCCAGGATAGGCTT142260.1595101806313199No Hit
TGTCAGTTTGTCAAATACCCCA141480.15863559929508744No Hit
TTCAAGTAATCCAGGATAGGC137920.15464392037587263No Hit
CAAAGAATTCTCCTTTTGGGCT137850.15456543230723638No Hit
TAGCTTATCAGACTGATGTTGACA137130.15375812645840642No Hit
TGTAAACATCCCCGACTGGAAGC132680.14876852780938793No Hit
TATTGCACTTGTCCCGGCCTGTT129870.14561779248270432No Hit
CTCCCACTGCTTCACTTGACTAGCCT126350.14167096388842448No Hit
AACATTCAACGCTGTCGGTGA123230.13817263854349468No Hit
ACTGGACTTGGAGTCAGAAGG121760.1365243891021335No Hit
ACTGGACTTGGAGTCAGAAGGCA119260.13372124379369613No Hit
AGCAGCATTGTACAGGGCTATGT117590.13184874272765995No Hit
ATCCCACCGCTGCCACCA116920.13109749978499874No Hit
ACTGGACTTGGAGTCAGAAGGCT113890.12770008767117266No Hit
TGAGGTAGTAGGTTGTATAGT112080.125670610467864No Hit
GCGTTCGCGCTTTCCCCTG111020.12448207685708658No Hit
TCGTTTCCCGGCCAATGCACCA110970.12442601395091782No Hit
ATCCCGGACGAGCCCCCA110230.12359628293962036No Hit
GGAATACCGGGTGCTGTAGGCTTT110140.1234953697085166No Hit
ACCACAGGGTAGAACCACGGAC109790.12310292936533539No Hit
TGTAAACATCCTACACTCAGCT109420.12268806385968667No Hit
AGCAGCATTGTACAGGGCTATG107280.12028857147566427No Hit
AACATTCAACGCTGTCGGTGAGA106540.1194588404643668No Hit
TTATAAAGCAATGAGACTGATT106140.11901033721501685No Hit
CACTAGATTGTGAGCTCCTGGA105170.11792271683534314No Hit
TAGCAGCACGTAAATATTGGCGT104900.1176199771420319No Hit
CAAAGTGCTGTTCGTGCAGGT103020.115512011870087No Hit
CGCGACCTCAGATCAGACGTGGCG102850.11532139798911326No Hit
CTCCCACTGCTTCACTTGACTA100380.11255189042437715No Hit
AGAGGTAGTAGGTTGCATAGTT99530.11159882101950842No Hit
TTCACAGTGGCTAAGTTCTG98040.10992814641567976No Hit
TTCACAGTGGCTAAGTTCTGC95390.10695681238873617No Hit
GGAATACCGGGTGCTGTAGGCTT95370.10693438722626865No Hit
CAAAGAATTCTCCTTTTGGGCTT92940.10420972998646752No Hit
TATTGCACTTGTCCCGGCCTGTAG90960.10198963890218514No Hit
CACCCGGCTGTGTGCACATGTGC90170.10110384498471893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)