FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10742171
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG128367211.949837700405254No Hit
CAACGGAATCCCAAAAGCAGCTG9063208.437028232002637No Hit
GCCTGTCTGAGCGTCGCT8492177.905450397317265No Hit
CCCCCACTGCTAAATTTGACTG4179103.8903681574236715No Hit
TTCAAGTAATCCAGGATAGGCT3846923.5811383006284294No Hit
AAGCTGCCAGTTGAAGAACTGT3629533.3787676625144023No Hit
TAGCAGCACGTAAATATTGGCG2856512.6591552117351323No Hit
AACATTCAACGCTGTCGGTGAGT2538252.3628836293892546No Hit
AACATTCAACGCTGTCGGTGAG1906651.7749205444597744No Hit
TAGCTTATCAGACTGATGTTGAC1659691.5450228822460563No Hit
TGAGGTAGTAGGTTGTATAGTT1434981.3358379791198634No Hit
TAGCTTATCAGACTGATGTTGACT1428281.329600878630586No Hit
TAGCTTATCAGACTGATGTTGA1341141.2484813358491502No Hit
TATTGCACTTGTCCCGGCCTGTAA1157641.077659255284616No Hit
TATTGCACTTGTCCCGGCCTGTAT1076981.0025720126778843No Hit
TATTGCACTTGTCCCGGCCTGT907800.8450805707710294No Hit
CATAAAGTAGAAAGCACTACT870820.8106554997123021No Hit
TGTAAACATCCTTGACTGGAAGCT835360.7776454126451721No Hit
TGAGGTAGTAGATTGTATAGTT822960.7661021221874051No Hit
TTCAAGTAATTCAGGATAGGTT786450.732114579073448No Hit
TCCTGTCTGAGCGTCGCT773180.7197613964625958No Hit
AACCGGGCGGAAACACCA648280.6034906724162183No Hit
CAACGGAATCCCAAAAGCAGCT633880.5900855609168761No Hit
AGCAGCATTGTACAGGGCTATGA610310.5681439999419111No Hit
TATTGCACTTGTCCCGGCCTGTA592930.5519647750906218No Hit
CAACGGAATCCCAAAAGCAGCTGT521990.4859259827459459No Hit
CCCATAAAGTAGAAAGCACTAC459610.4278557844592122No Hit
ACTGGACTTGGAGTCAGAAGGC450590.41945897156170764No Hit
TCGTACGACTCTTAGCGG432790.4028887642916874No Hit
CGCCTGTCTGAGCGTCGCT422800.3935889681890188No Hit
TGTAAACATCCCCGACTGGAAGCT419740.39074038199540856No Hit
CAACACCAGTCGATGGGCTGT393230.3660619440893279No Hit
CATTGCACTTGTCTCGGTCTGA392420.36530790656748996No Hit
CCCATAAAGTAGAAAGCACT365290.340052304138521No Hit
CTCCCACTGCTTCACTTGACTAG325810.3032999567778245No Hit
TTCTCACTACTGCACTTGACTAG318970.296932528815637No Hit
TGAGGTAGTAGTTTGTACAGTT311520.289997245435769No Hit
TGTAAACATCCTACACTCTCAGCT286400.26661277315358317No Hit
ACCCCCCACTGCTAAATTTGACTG279910.26057116387367135No Hit
CTTCTCACTACTGCACTTGACTAG261270.24321899176618952No Hit
TCGTTTCCCGGCCAATGCACCA254910.23729840085398007No Hit
TGAGGTAGTAGTTTGTGCTGTT249520.23228079314693462No Hit
AACGGAATCCCAAAAGCAGCTG244500.22760762233258064No Hit
CACCCGGCTGTGTGCACATGTGC235470.21920150032986815No Hit
TAGCTTATCAGACTGATGTTGACA211340.19673862946326215No Hit
CCCCCCACTGCTAAATTTGACTGG206390.19213062238536324No Hit
TAGCAGCACGTAAATATTGGC193330.17997293098387654No Hit
ACCGGGTGCTGTAGGCTT190320.1771708903163057No Hit
TTCAAGTAATCCAGGATAGGCTT189070.17600725216532115No Hit
TTCAAGTAATCCAGGATAGGC188540.17551386958930368No Hit
TTCACAGTGGCTAAGTTCCGC186640.17374513959980714No Hit
TTCACAGTGGCTAAGTTCCG179600.16719152953346209No Hit
TTCACAGTGGCTAAGTTCTGC178080.16577654554186486No Hit
AGCTCGGTCTGAGGCCCCTCAGT175040.1629465775586704No Hit
CAAAGAATTCTCCTTTTGGGCT171390.15954875415779549No Hit
CCCCCACTGCTAAATTTGACTGGCTT170260.15849682526930545No Hit
TGATGTAGTAGGTTGTATAGTT165600.15415878224243498No Hit
CCCCCCACTGCTAAATTTGACTGT161650.15048168568532377No Hit
CACTAGATTGTGAGCTCCTGGA158180.14725142617819062No Hit
AACATTCAACGCTGTCGGTGAGTT156650.1458271330813855No Hit
TGAGGTAGTAGGTTGTGTGGTT153870.14323920183359584No Hit
TATTGCACTTGTCCCGGCCTGTT152830.1422710548919767No Hit
CAACGGAATCCCAAAAGCAGCTGA152290.14176836321075137No Hit
CCCCCCACTGCTAAATTTGACTGGCT149540.1392083592785853No Hit
TTATAAAGCAATGAGACTGATT146720.13658319160996413No Hit
ACCGGGTGCTGTAGGCTTT135050.12571946583237226No Hit
AATGCACCCGGGCAAGGATTCT133870.12462099141784282No Hit
AGCAGCATTGTACAGGGCTATGT132440.12328978937311648No Hit
TATTGCACTTGTCCCGGCCTGTAG128870.11996643881390456No Hit
CCCCCCACTGCTAAATTTGACTGTA128340.1194730562378871No Hit
ACCGGGTGCTGTAGGCTTA127930.11909138292436416No Hit
CTACGCCTGTCTGAGCGTCGCT127840.11900760097749329No Hit
CAAAGTGCTGTTCGTGCAGGT127280.1184862910858522No Hit
TTCACAGTGGCTAAGTTCTG127230.11843974555981282No Hit
CACTAGATTGTGAGCTCCTGGAA125650.11696890693696832No Hit
CAAAGAATTCTCCTTTTGGGCTT124610.11600075999534916No Hit
TGAGGTAGTAGGTTGTATAGT122900.11440890300480229No Hit
TGTAAACATCCCCGACTGGAAGC118720.11051769702790991No Hit
TGTCAGTTTGTCAAATACCCCAA118360.11018256924042635No Hit
CCCATAAAGTAGAAAGCACTA117440.10932613156130172No Hit
ACCACAGGGTAGAACCACGGAC114650.10672889120830417No Hit
TGTCAGTTTGTCAAATACCCCA114260.10636583610519698No Hit
AATGACACGATCACTCCCGTTG111160.10348001349075525No Hit
TGTAAACATCCTACACTCAGCT110370.10274459417933303No Hit
TGAGGTAGTAAGTTGTATTGTT110090.10248393923351247No Hit
AGCAGCATTGTACAGGGCTATG109380.10182299276375324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)