FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321647
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG88260312.054705723998985No Hit
CAACGGAATCCCAAAAGCAGCTG6712249.167664051544687No Hit
CCCCCACTGCTAAATTTGACTG2993544.0886155806200435No Hit
AAGCTGCCAGTTGAAGAACTGT2650253.619745666514652No Hit
TTCAAGTAATCCAGGATAGGCT2514443.4342546151159703No Hit
TAGCAGCACGTAAATATTGGCG2424993.3120826502561513No Hit
GCCTGTCTGAGCGTCGCT1414211.9315462764047486No Hit
AACATTCAACGCTGTCGGTGAGT1295921.769984267201082No Hit
AACATTCAACGCTGTCGGTGAG1146121.5653854931820668No Hit
TAGCTTATCAGACTGATGTTGAC963821.316397799566136No Hit
TAGCTTATCAGACTGATGTTGACT834201.1393611300845288No Hit
TAGCTTATCAGACTGATGTTGA809461.1055709186744458No Hit
TATTGCACTTGTCCCGGCCTGTAA742161.0136517097860631No Hit
CATAAAGTAGAAAGCACTACT736251.0055797554839778No Hit
TATTGCACTTGTCCCGGCCTGTAT693400.9470546722615827No Hit
TGAGGTAGTAGGTTGTATAGTT682540.9322219440516594No Hit
TGTAAACATCCTTGACTGGAAGCT582830.7960367387283217No Hit
TATTGCACTTGTCCCGGCCTGT529260.7228701411034976No Hit
CCCATAAAGTAGAAAGCACTAC442380.6042083154241116No Hit
TGAGGTAGTAGATTGTATAGTT435150.5943334880799361No Hit
CAACGGAATCCCAAAAGCAGCTGT419250.5726170628002143No Hit
AACCGGGCGGAAACACCA409030.5586584548531225No Hit
TTCAAGTAATTCAGGATAGGTT371560.5074814450901552No Hit
TATTGCACTTGTCCCGGCCTGTA369260.5043400753956042No Hit
TTAATGCTAATCGTGATAGGGGTT358510.48965758660585523No Hit
TTCTCACTACTGCACTTGACTAG345420.4717790956051282No Hit
AGCAGCATTGTACAGGGCTATGA324670.44343847770863576No Hit
TCGTACGACTCTTAGCGG288460.39398239221311815No Hit
CTCCCACTGCTTCACTTGACTAG271500.37081820524808146No Hit
TGTAAACATCCCCGACTGGAAGCT254470.3475584113792976No Hit
CCCCCACTGCTAAATTTGACTGGCTT246150.3361948479624871No Hit
ACTGGACTTGGAGTCAGAAGGC244480.333913940401661No Hit
CCCATAAAGTAGAAAGCACT242200.33079988696532353No Hit
CTTCTCACTACTGCACTTGACTAG233240.31856220328568147No Hit
ACCCCCCACTGCTAAATTTGACTG226180.30891956413632066No Hit
CATTGCACTTGTCTCGGTCTGA220120.30064273789763424No Hit
TGAGAACTGAATTCCATGGGTT214440.29288492056500404No Hit
TGTAAACATCCTACACTCTCAGCT213840.2920654328185994No Hit
GGAATACCGGGTGCTGTAGGCTTT213500.29160105642897016No Hit
TAGCAGCACGTAAATATTGGC178260.24346980945680663No Hit
CCCCCCACTGCTAAATTTGACTGG171680.23448276050456954No Hit
TGAGGTAGTAGTTTGTACAGTT168590.23026239861058587No Hit
CAAAGAATTCTCCTTTTGGGCT163710.22359723160649508No Hit
CAACGGAATCCCAAAAGCAGCT163040.22268213695634329No Hit
GGAATACCGGGTGCTGTAGGCTT157530.21515650781852771No Hit
CAACACCAGTCGATGGGCTGT150760.20590995441326249No Hit
AACGGAATCCCAAAAGCAGCTG150150.20507680853775112No Hit
TTCACAGTGGCTAAGTTCCGC140670.19212890214455847No Hit
TAGCTTATCAGACTGATGTTGACA138630.1893426438067828No Hit
TTCAAGTAATCCAGGATAGGC133790.18273210931911904No Hit
CCCCCCACTGCTAAATTTGACTGGCT133240.18198091221824816No Hit
TGAGGTAGTAGTTTGTGCTGTT132090.18041022737097268No Hit
TGAGAACTGAATTCCATAGGCTGT127590.1742640692729382No Hit
CACCCGGCTGTGTGCACATGTGC126200.17236558932710086No Hit
TGATGTAGTAGGTTGTATAGTT124930.17063100693054445No Hit
TTCACAGTGGCTAAGTTCCG123130.16817254369133067No Hit
AGCTCGGTCTGAGGCCCCTCAGT119720.16351512166593118No Hit
TGTCAGTTTGTCAAATACCCCA119390.16306440340540865No Hit
CGCCTGTCTGAGCGTCGCT119180.16277758269416703No Hit
CCCCCCACTGCTAAATTTGACTGT115810.15817479318519453No Hit
TGTCAGTTTGTCAAATACCCCAA110140.15043063398167106No Hit
ATCACATTGCCAGGGATTTCC106140.14496738233897374No Hit
CCCATAAAGTAGAAAGCACTA106010.14478982666058607No Hit
CCCCCCACTGCTAAATTTGACTGTA105850.14457129659487816No Hit
ACCGGGTGCTGTAGGCTT102210.13959973760002362No Hit
TTCACAGTGGCTAAGTTCTGC102030.13935389127610223No Hit
TTCAAGTAATCCAGGATAGGCTT100320.13701835119884911No Hit
CACTAGATTGTGAGCTCCTGGA92820.12677475436879163No Hit
ATCCCGGACGAGCCCCCA92650.12654256617397697No Hit
CAAAGAATTCTCCTTTTGGGCTT92470.12629671985005558No Hit
TATTGCACTTGTCCCGGCCTGTT91600.12510846261776892No Hit
TGTAAACATCCTACACTCAGCT89610.12239049492552699No Hit
TCCCACTGCTTCACTTGACTAG89520.1222675717635663No Hit
TGTAAACATCCCCGACTGGAAGC87860.12000032233184692No Hit
TCGTTTCCCGGCCAATGCACCA87570.11960423658775136No Hit
TATTGCACTTGTCCCGGCCTGTAG87110.11897596264884118No Hit
CTACGCCTGTCTGAGCGTCGCT85270.1164628668932004No Hit
CACTAGATTGTGAGCTCCTGGAA83630.11422293371969448No Hit
TCCTGTCTGAGCGTCGCT82800.11308930900383479No Hit
TTATAAAGCAATGAGACTGATT80290.10966111859804221No Hit
AACATTCAACGCTGTCGGTGAGTT79310.10832262194558136No Hit
CTCCCACTGCTTCACTTGACTAGCCT78430.10712070658418794No Hit
ACCGGGTGCTGTAGGCTTA78010.10654706516170474No Hit
TGATGTAGTAGATTGTATAGTT77610.106000739997435No Hit
TAGCAGCACGTAAATATTGGCGT77440.10576855180262036No Hit
ACCGGGTGCTGTAGGCTTT74750.10209451507290641No Hit
ATCACATTGCCAGGGATTTCCA74300.10147989926310295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)