FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11725302
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG151263112.90057177205329No Hit
CAACGGAATCCCAAAAGCAGCTG7762496.620290036026365No Hit
AAGCTGCCAGTTGAAGAACTGT4426703.775339859050112No Hit
CCCCCACTGCTAAATTTGACTG3970503.3862667247291367No Hit
AACATTCAACGCTGTCGGTGAGT3551043.028527538139316No Hit
TAGCAGCACGTAAATATTGGCG3242152.7650886945172073No Hit
TTCAAGTAATCCAGGATAGGCT3070812.61896026217491No Hit
TAGCTTATCAGACTGATGTTGAC2578402.1990051940666433No Hit
TAGCTTATCAGACTGATGTTGACT2027441.729115378009027No Hit
TATTGCACTTGTCCCGGCCTGTAA1899941.6203761745326475No Hit
AACATTCAACGCTGTCGGTGAG1855401.582389946118232No Hit
TATTGCACTTGTCCCGGCCTGTAT1720531.467365190252669No Hit
TGTAAACATCCTTGACTGGAAGCT1515281.2923163940681441No Hit
TAGCTTATCAGACTGATGTTGA1293091.1028202088099734No Hit
GCCTGTCTGAGCGTCGCT1205261.0279138226034605No Hit
TTAATGCTAATCGTGATAGGGGTT1166510.9948656333116196No Hit
AGCAGCATTGTACAGGGCTATGA1017720.8679691149959293No Hit
TATTGCACTTGTCCCGGCCTGT868620.7408082111659043No Hit
TGTAAACATCCCCGACTGGAAGCT847520.7228129390611858No Hit
TATTGCACTTGTCCCGGCCTGTA767840.6548573333121824No Hit
TGAGGTAGTAGGTTGTATAGTT745370.635693647805404No Hit
TGTAAACATCCTACACTCTCAGCT641790.5473547717576912No Hit
ACTGGACTTGGAGTCAGAAGGC600220.5119015271419022No Hit
CATAAAGTAGAAAGCACTACT586380.5000979932116034No Hit
CAACGGAATCCCAAAAGCAGCTGT532860.45445311344645967No Hit
TGAGGTAGTAGATTGTATAGTT525740.4483807751817394No Hit
CCCATAAAGTAGAAAGCACTAC496940.4238185080435454No Hit
TTCAAGTAATTCAGGATAGGTT482070.4111365319204571No Hit
CGCCTGTCTGAGCGTCGCT423360.3610653269314513No Hit
TAGCTTATCAGACTGATGTTGACA405890.3461659239139427No Hit
CTTCTCACTACTGCACTTGACTAG396410.33808084431428714No Hit
AACCGGGCGGAAACACCA396400.3380723157493086No Hit
TTCTCACTACTGCACTTGACTAG377030.3215524853858775No Hit
ACCCCCCACTGCTAAATTTGACTG365240.3114973072761793No Hit
TCCTGTCTGAGCGTCGCT354130.30202207158502187No Hit
CAAAGAATTCTCCTTTTGGGCT336380.2868838687481141No Hit
AACATTCAACGCTGTCGGTGAGTT322930.27541294885197837No Hit
CCCCCACTGCTAAATTTGACTGGCTT310170.2645304999393619No Hit
CCCCCCACTGCTAAATTTGACTGTA288710.24622819949541597No Hit
CCCCCCACTGCTAAATTTGACTGG270340.23056122562983877No Hit
CCCATAAAGTAGAAAGCACT267370.2280282418312125No Hit
CAAAGAATTCTCCTTTTGGGCTT265190.22616901466589093No Hit
TCGTACGACTCTTAGCGG260840.22245908890022617No Hit
CTACGCCTGTCTGAGCGTCGCT257680.21976406236700768No Hit
CATTGCACTTGTCTCGGTCTGA252810.21561065122245893No Hit
TGTAAACATCCCCGACTGGAAGC250580.2137087812322446No Hit
CCCCCCACTGCTAAATTTGACTGT239210.2040118028516451No Hit
AGCTCGGTCTGAGGCCCCTCAGT239200.20400327428666656No Hit
TATTGCACTTGTCCCGGCCTGTAG236990.20211846142640932No Hit
TTAATGCTAATCGTGATAGGGGTTT235200.20059184829525073No Hit
CAACGGAATCCCAAAAGCAGCT233760.199363734938341No Hit
TGTAAACATCCTACACTCAGCT230320.19642990858572343No Hit
TGTCAGTTTGTCAAATACCCCA225610.19241295448083126No Hit
CCCCCCACTGCTAAATTTGACTGGCT225040.19192682627705454No Hit
CTCCCACTGCTTCACTTGACTAG221450.18886507144975884No Hit
CACTAGATTGTGAGCTCCTGGAA218090.18599947361696953No Hit
TGAGGTAGTAGTTTGTACAGTT216640.1847628316950813No Hit
TTCAAGTAATCCAGGATAGGCTT215970.18419141784151913No Hit
TGTCAGTTTGTCAAATACCCCAA213720.1822724907213477No Hit
TAGCAGCACGTAAATATTGGC205350.17513408183431012No Hit
ATCACATTGCCAGGGATTTCCA195740.16693813088993356No Hit
ACTGGACTTGGAGTCAGAAGGCA194340.16574413179293804No Hit
AGCAGCATTGTACAGGGCTATGT193930.16539446062881794No Hit
CACTAGATTGTGAGCTCCTGGA188900.1611045924446125No Hit
TGATGTAGTAGGTTGTATAGTT187610.1600044075623809No Hit
TGAGGTAGTAGTTTGTGCTGTT185270.15800872335740265No Hit
TACGCCTGTCTGAGCGTCGCT184030.15695118130006375No Hit
CACCCGGCTGTGTGCACATGTGC177280.15119439993954953No Hit
TATTGCACTTGTCCCGGCCTGTT174110.1484908448413525No Hit
TATTGCACTTGTCCCGGCCTGTAAT170500.1454120328840997No Hit
TAGCAGCACGTAAATATTGGCGT170270.14521587588959328No Hit
GGAATACCGGGTGCTGTAGGCTTT164870.14061045080118192No Hit
TCGTTTCCCGGCCAATGCACCA158410.13510099782504537No Hit
TTATAAAGCAATGAGACTGATT149160.12721207521989625No Hit
CCCCCCACTGCTAAATTTGACTGA148320.12649567576169893No Hit
TATTGCACTTGTCCCGGCCTGTAAA146210.12469614855122708No Hit
ACTTGACTTGGAGTCAGAAGGC143850.12268340721629173No Hit
ACGCCTGTCTGAGCGTCGCT143720.12257253587157073No Hit
TTCACAGTGGCTAAGTTCCG142410.12145529385938206No Hit
TATTGCACTTGTCCCGGCCTGTTT141730.12087535144084136No Hit
ACTGGACTTGGAGTCAGAAGGCT140280.11963870951895311No Hit
AACGGAATCCCAAAAGCAGCTG139280.11878585302109916No Hit
GGGCTACGCCTGTCTGAGCGTCGCT133450.11381369963861059No Hit
ATCACATTGCCAGGGATTTCC133160.11356637125423295No Hit
CCAAGTCCTTCTGATCGAGGCCC132490.1129949574006708No Hit
TTCAAGTAATCCAGGATAGGC131790.112397957852173No Hit
TGATGTAGTAGATTGTATAGTT130300.11112720167037063No Hit
CAACACCAGTCGATGGGCTGT130250.11108455884547794No Hit
ACCACAGGGTAGAACCACGGAC127570.10879890343122932No Hit
TCGTTTCCCGGGCGGCGCACCA125790.10728081886504928No Hit
CAGTGCAATAGTATTGTCAAAGC123970.10572862003895508No Hit
TGAGAACTGAATTCCATAGGCTGT122860.10478194932633718No Hit
CAAAGTGCTGTTCGTGCAGGTAG121800.10387792143861198No Hit
AGCAGCATTGTACAGGGCTATG121460.10358795022934164No Hit
TGTAAACATCCTTGACTGGAAGC120960.10316152198041467No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT120190.10250482247706712No Hit
TGTCAGTTTGTCAAATACCCC119830.10219779413783969No Hit
ATGCACCTGGGCAAGGATTCTGA119500.10191635149354789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)