FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6165725
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG64839810.51616800943928No Hit
CAACGGAATCCCAAAAGCAGCTG64452510.453353012013997No Hit
CCCCCACTGCTAAATTTGACTG2527954.100004460140535No Hit
GCCTGTCTGAGCGTCGCT2463143.9948911117508485No Hit
TAGCAGCACGTAAATATTGGCG2312433.750459191741442No Hit
TTCAAGTAATCCAGGATAGGCT2103213.4111316998406513No Hit
AAGCTGCCAGTTGAAGAACTGT1940603.1473995353344497No Hit
AACATTCAACGCTGTCGGTGAGT1188111.926959116729987No Hit
AACATTCAACGCTGTCGGTGAG1019651.6537390169039328No Hit
TAGCTTATCAGACTGATGTTGAC880681.4283478423056493No Hit
TAGCTTATCAGACTGATGTTGA784211.271886112338776No Hit
TAGCTTATCAGACTGATGTTGACT742121.2036216341143986No Hit
TGAGGTAGTAGGTTGTATAGTT684591.110315494122751No Hit
CATAAAGTAGAAAGCACTACT630741.0229778331015411No Hit
TTAATGCTAATCGTGATAGGGGTT545050.8839998540317643No Hit
TATTGCACTTGTCCCGGCCTGTAA519370.8423502507815382No Hit
TCGTACGACTCTTAGCGG509860.8269262738769569No Hit
TATTGCACTTGTCCCGGCCTGTAT457970.7427674766552189No Hit
TGTAAACATCCTTGACTGGAAGCT449930.7297276476002417No Hit
TATTGCACTTGTCCCGGCCTGT439200.7123249901674175No Hit
TGAGGTAGTAGATTGTATAGTT363350.5893062048664188No Hit
CAACGGAATCCCAAAAGCAGCTGT349300.5665189414059174No Hit
CCCATAAAGTAGAAAGCACTAC337470.5473322277591038No Hit
TTCAAGTAATTCAGGATAGGTT323430.5245611829914568No Hit
TATTGCACTTGTCCCGGCCTGTA320050.5190792648066529No Hit
AGCAGCATTGTACAGGGCTATGA308670.5006223923382895No Hit
CCCATAAAGTAGAAAGCACT274440.4451058066975092No Hit
AACCGGGCGGAAACACCA238600.38697801150716254No Hit
TGAGAACTGAATTCCATAGGCTGT223160.3619363497398927No Hit
ACTGGACTTGGAGTCAGAAGGC216400.3509725133702849No Hit
CATTGCACTTGTCTCGGTCTGA214060.34717733924234373No Hit
CAACGGAATCCCAAAAGCAGCT211850.34359300812151045No Hit
TGTAAACATCCCCGACTGGAAGCT208780.3386138694151945No Hit
TTCTCACTACTGCACTTGACTAG186250.30207315441412No Hit
CAACACCAGTCGATGGGCTGT184410.29908891492890133No Hit
TGTAAACATCCTACACTCTCAGCT161530.2619805456779211No Hit
TAGCAGCACGTAAATATTGGC161150.26136423534945197No Hit
CCCCCACTGCTAAATTTGACTGGCTT161030.26116961103519865No Hit
ACCCCCCACTGCTAAATTTGACTG159990.2594828669783359No Hit
TTCACAGTGGCTAAGTTCCG154000.24976786995852068No Hit
TGAGAACTGAATTCCATGGGTT148880.2414638992170426No Hit
CAAAGAATTCTCCTTTTGGGCT143090.23207327605431638No Hit
TCCTGTCTGAGCGTCGCT143030.2319759638971897No Hit
AACGGAATCCCAAAAGCAGCTG142690.23142452834013844No Hit
TGAGGTAGTAGTTTGTACAGTT139160.2256993297625178No Hit
CTTCTCACTACTGCACTTGACTAG138810.22513167551261207No Hit
TAGCTTATCAGACTGATGTTGACA129970.21079435102927876No Hit
TGATGTAGTAGGTTGTATAGTT120760.1958569349103309No Hit
ACCGGGTGCTGTAGGCTT118340.19193201123955414No Hit
CCCATAAAGTAGAAAGCACTA115110.18669337344756698No Hit
TGAGGTAGTAGTTTGTGCTGTT112640.18268735631251798No Hit
CCCCCCACTGCTAAATTTGACTGG105790.17157755170722017No Hit
CGCCTGTCTGAGCGTCGCT105450.17102611615016888No Hit
TTCAAGTAATCCAGGATAGGC103970.16862574960771037No Hit
CTCCCACTGCTTCACTTGACTAG101350.16437645207984464No Hit
CACCCGGCTGTGTGCACATGTGC90770.14721707503983716No Hit
TCGTTTCCCGGCCAATGCACCA90710.14711976288271045No Hit
CACTAGATTGTGAGCTCCTGGA88730.1439084616975295No Hit
TTCACAGTGGCTAAGTTCCGC85450.1385887304412701No Hit
AACATTCAACGCTGTCGGTGAGTT85230.13823191919847222No Hit
CCCCCCACTGCTAAATTTGACTGGCT84590.13719392285578744No Hit
AGCTCGGTCTGAGGCCCCTCAGT84270.13667492468444506No Hit
TTAATGCTAATCGTGATAGGGGT82820.13432321422054988No Hit
TATTGCACTTGTCCCGGCCTGTT81490.13216612807090813No Hit
CCCCCCACTGCTAAATTTGACTGTA81160.13163091120671128No Hit
TTCACAGTGGCTAAGTTCTG78750.127722206228789No Hit
ACCGGGTGCTGTAGGCTTT78490.1273005202145733No Hit
TTCACAGTGGCTAAGTTCTGC77800.12618143040761629No Hit
ATCACATTGCCAGGGATTTCC76930.1247704041292792No Hit
TTCAAGTAATCCAGGATAGGCTT75450.12237003758682069No Hit
TGTCAGTTTGTCAAATACCCCA74140.12024538882288782No Hit
TTATAAAGCAATGAGACTGATT73180.11868839430886066No Hit
CAAAGAATTCTCCTTTTGGGCTT72500.11758552319475811No Hit
CCCCCCACTGCTAAATTTGACTGT72500.11758552319475811No Hit
GGAATACCGGGTGCTGTAGGCTTT71820.11648265208065556No Hit
TTAATGCTAATCGTGATAGGGGTTT70890.11497431364519177No Hit
CACTAGATTGTGAGCTCCTGGAA70740.11473103325237502No Hit
AGCTACATTGTCTGCTGGGTTT68880.11171435638144744No Hit
ACCGGGTGCTGTAGGCTTA68690.11140620121721291No Hit
TGTAAACATCCTACACTCAGCT68060.11038442356738258No Hit
ATCCCGGACGAGCCCCCA67400.10931398983898893No Hit
AGCAGCATTGTACAGGGCTATG65670.10650815597516919No Hit
CTACGCCTGTCTGAGCGTCGCT64910.10527553531823103No Hit
TGTAAACATCCCCGACTGGAAGC64440.10451325675407191No Hit
TGAGGTAGTAGGTTGTGTGGTT64000.10379963426847612No Hit
TGATGTAGTAGATTGTATAGTT63390.10281029400435472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)